Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision Medicine
Molecular and genetic differences between individual cells within tissues underlie cellular heterogeneities defining organ physiology and function in homeostasis as well as in disease states. Transcriptional control of endogenous gene expression has been intensively studied for decades. Thanks to a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Cardiovascular Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2021.783072/full |
| _version_ | 1798026047031083008 |
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| author | Eric Schoger Eric Schoger Eric Schoger Sara Lelek Sara Lelek Daniela Panáková Daniela Panáková Laura Cecilia Zelarayán Laura Cecilia Zelarayán Laura Cecilia Zelarayán |
| author_facet | Eric Schoger Eric Schoger Eric Schoger Sara Lelek Sara Lelek Daniela Panáková Daniela Panáková Laura Cecilia Zelarayán Laura Cecilia Zelarayán Laura Cecilia Zelarayán |
| author_sort | Eric Schoger |
| collection | DOAJ |
| description | Molecular and genetic differences between individual cells within tissues underlie cellular heterogeneities defining organ physiology and function in homeostasis as well as in disease states. Transcriptional control of endogenous gene expression has been intensively studied for decades. Thanks to a fast-developing field of single cell genomics, we are facing an unprecedented leap in information available pertaining organ biology offering a comprehensive overview. The single-cell technologies that arose aided in resolving the precise cellular composition of many organ systems in the past years. Importantly, when applied to diseased tissues, the novel approaches have been immensely improving our understanding of the underlying pathophysiology of common human diseases. With this information, precise prediction of regulatory elements controlling gene expression upon perturbations in a given cell type or a specific context will be realistic. Simultaneously, the technological advances in CRISPR-mediated regulation of gene transcription as well as their application in the context of epigenome modulation, have opened up novel avenues for targeted therapy and personalized medicine. Here, we discuss the fast-paced advancements during the recent years and the applications thereof in the context of cardiac biology and common cardiac disease. The combination of single cell technologies and the deep knowledge of fundamental biology of the diseased heart together with the CRISPR-mediated modulation of gene regulatory networks will be instrumental in tailoring the right strategies for personalized and precision medicine in the near future. In this review, we provide a brief overview of how single cell transcriptomics has advanced our knowledge and paved the way for emerging CRISPR/Cas9-technologies in clinical applications in cardiac biomedicine. |
| first_indexed | 2024-04-11T18:28:53Z |
| format | Article |
| id | doaj.art-96c1f2ddc3ee48aea3d9ff950720cfb0 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-11T18:28:53Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-96c1f2ddc3ee48aea3d9ff950720cfb02022-12-22T04:09:31ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-01-01810.3389/fcvm.2021.783072783072Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision MedicineEric Schoger0Eric Schoger1Eric Schoger2Sara Lelek3Sara Lelek4Daniela Panáková5Daniela Panáková6Laura Cecilia Zelarayán7Laura Cecilia Zelarayán8Laura Cecilia Zelarayán9Institute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, GermanyDZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Goettingen, GermanyCluster of Excellence “Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells”, University of Goettingen, Goettingen, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, GermanyDZHK (German Center for Cardiovascular Research), Partner Site Berlin, Berlin, GermanyMax Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, GermanyDZHK (German Center for Cardiovascular Research), Partner Site Berlin, Berlin, GermanyInstitute of Pharmacology and Toxicology, University Medical Center Goettingen, Goettingen, GermanyDZHK (German Center for Cardiovascular Research), Partner Site Goettingen, Goettingen, GermanyCluster of Excellence “Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells”, University of Goettingen, Goettingen, GermanyMolecular and genetic differences between individual cells within tissues underlie cellular heterogeneities defining organ physiology and function in homeostasis as well as in disease states. Transcriptional control of endogenous gene expression has been intensively studied for decades. Thanks to a fast-developing field of single cell genomics, we are facing an unprecedented leap in information available pertaining organ biology offering a comprehensive overview. The single-cell technologies that arose aided in resolving the precise cellular composition of many organ systems in the past years. Importantly, when applied to diseased tissues, the novel approaches have been immensely improving our understanding of the underlying pathophysiology of common human diseases. With this information, precise prediction of regulatory elements controlling gene expression upon perturbations in a given cell type or a specific context will be realistic. Simultaneously, the technological advances in CRISPR-mediated regulation of gene transcription as well as their application in the context of epigenome modulation, have opened up novel avenues for targeted therapy and personalized medicine. Here, we discuss the fast-paced advancements during the recent years and the applications thereof in the context of cardiac biology and common cardiac disease. The combination of single cell technologies and the deep knowledge of fundamental biology of the diseased heart together with the CRISPR-mediated modulation of gene regulatory networks will be instrumental in tailoring the right strategies for personalized and precision medicine in the near future. In this review, we provide a brief overview of how single cell transcriptomics has advanced our knowledge and paved the way for emerging CRISPR/Cas9-technologies in clinical applications in cardiac biomedicine.https://www.frontiersin.org/articles/10.3389/fcvm.2021.783072/fullsingle cell sequencingCRIPSR/Cas9 systemendogenous gene activationcardiomyocytesgene regulation |
| spellingShingle | Eric Schoger Eric Schoger Eric Schoger Sara Lelek Sara Lelek Daniela Panáková Daniela Panáková Laura Cecilia Zelarayán Laura Cecilia Zelarayán Laura Cecilia Zelarayán Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision Medicine Frontiers in Cardiovascular Medicine single cell sequencing CRIPSR/Cas9 system endogenous gene activation cardiomyocytes gene regulation |
| title | Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision Medicine |
| title_full | Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision Medicine |
| title_fullStr | Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision Medicine |
| title_full_unstemmed | Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision Medicine |
| title_short | Tailoring Cardiac Synthetic Transcriptional Modulation Towards Precision Medicine |
| title_sort | tailoring cardiac synthetic transcriptional modulation towards precision medicine |
| topic | single cell sequencing CRIPSR/Cas9 system endogenous gene activation cardiomyocytes gene regulation |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2021.783072/full |
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