Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients
Protease-resistant protein and lipid mycobacterial antigens are present in biological fluids of pulmonary tuberculosis patients. Anti-tuberculosis monoclonal antibodies (MAb) reacting to proteins participating in the molybdopterin synthesis have been obtained. With the help of two MAb recognizing di...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
New Terra Publishing House
2016-02-01
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| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/850 |
| _version_ | 1797759465063186432 |
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| author | A. V. Onyan V. G. Аvdienko S. S. Babayan T. R. Bagdasaryan V. Ya. Gergert |
| author_facet | A. V. Onyan V. G. Аvdienko S. S. Babayan T. R. Bagdasaryan V. Ya. Gergert |
| author_sort | A. V. Onyan |
| collection | DOAJ |
| description | Protease-resistant protein and lipid mycobacterial antigens are present in biological fluids of pulmonary tuberculosis patients. Anti-tuberculosis monoclonal antibodies (MAb) reacting to proteins participating in the molybdopterin synthesis have been obtained. With the help of two MAb recognizing different epitopes on one antigen with molecular mass of 32, 50 and 75 kilodalton. With the help of which the two-site sandwich of IFA type capable of detecting the mycobacterial antigen in antigenic preparations of х M. tuberculosis and in urine samples of tuberculosis patients. However the specificity of this test is fairly low since when testing urine samples this antigen was found in the half of healthy donors. It is due to the fact that in human urogenital tract some opportunistic pathogens are present which belong to normal bacterial population with which the tested MAb have the cross-reaction. |
| first_indexed | 2024-03-12T18:44:51Z |
| format | Article |
| id | doaj.art-96c37f96dfa7453fb860bb60e0b63d53 |
| institution | Directory Open Access Journal |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| last_indexed | 2024-03-12T18:44:51Z |
| publishDate | 2016-02-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj.art-96c37f96dfa7453fb860bb60e0b63d532023-08-02T07:47:51ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062016-02-019424246850Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patientsA. V. Onyan0V. G. Аvdienko1S. S. Babayan2T. R. Bagdasaryan3V. Ya. Gergert4ФГБУН «Центральный НИИ туберкулеза»ФГБУН «Центральный НИИ туберкулеза»ФГБУН «Центральный НИИ туберкулеза»ФГБУН «Центральный НИИ туберкулеза»ФГБУН «Центральный НИИ туберкулеза»Protease-resistant protein and lipid mycobacterial antigens are present in biological fluids of pulmonary tuberculosis patients. Anti-tuberculosis monoclonal antibodies (MAb) reacting to proteins participating in the molybdopterin synthesis have been obtained. With the help of two MAb recognizing different epitopes on one antigen with molecular mass of 32, 50 and 75 kilodalton. With the help of which the two-site sandwich of IFA type capable of detecting the mycobacterial antigen in antigenic preparations of х M. tuberculosis and in urine samples of tuberculosis patients. However the specificity of this test is fairly low since when testing urine samples this antigen was found in the half of healthy donors. It is due to the fact that in human urogenital tract some opportunistic pathogens are present which belong to normal bacterial population with which the tested MAb have the cross-reaction.https://www.tibl-journal.com/jour/article/view/850микобактериальные антигенымоноклональные антителагибридомная технологиягибридомная технология |
| spellingShingle | A. V. Onyan V. G. Аvdienko S. S. Babayan T. R. Bagdasaryan V. Ya. Gergert Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients Туберкулез и болезни лёгких микобактериальные антигены моноклональные антитела гибридомная технология гибридомная технология |
| title | Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients |
| title_full | Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients |
| title_fullStr | Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients |
| title_full_unstemmed | Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients |
| title_short | Monoclonal antibodies against Mycobacterium tuberculosis, detecting the antigen in urine of the pulmonary tuberculosis patients |
| title_sort | monoclonal antibodies against mycobacterium tuberculosis detecting the antigen in urine of the pulmonary tuberculosis patients |
| topic | микобактериальные антигены моноклональные антитела гибридомная технология гибридомная технология |
| url | https://www.tibl-journal.com/jour/article/view/850 |
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