The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers
ABSTRACT: The present study investigated the individual and combined effects of xylo-oligosaccharides (XOS) and gamma-irradiated astragalus polysaccharides (IAPS) on the immune response, antioxidant capacity and intestinal microbiota composition of broiler chickens. A total of 240 newly hatched Ross...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122002875 |
| _version_ | 1798037052834447360 |
|---|---|
| author | Q. Wang X.F. Wang T. Xing J.L. Li X.D. Zhu L. Zhang F. Gao |
| author_facet | Q. Wang X.F. Wang T. Xing J.L. Li X.D. Zhu L. Zhang F. Gao |
| author_sort | Q. Wang |
| collection | DOAJ |
| description | ABSTRACT: The present study investigated the individual and combined effects of xylo-oligosaccharides (XOS) and gamma-irradiated astragalus polysaccharides (IAPS) on the immune response, antioxidant capacity and intestinal microbiota composition of broiler chickens. A total of 240 newly hatched Ross 308 chicks were randomly allocated into 5 dietary treatments including the basal diet (control), or the basal diet supplemented with 50 mg/kg chlortetracycline (CTC), 100 mg/kg XOS (XOS), 600 mg/kg IAPS (IAPS), and 100 mg/kg XOS + 600 mg/kg IAPS (XOS + IAPS) respectively. The results showed that birds in the control group had lower the thymus index and serum lysozyme activity than those in the other 4 groups (P < 0.05). Moreover, there was an interaction between XOS and IAPS treatments on increasing the serum lysozyme activity (P < 0.05). Birds in the CTC and XOS + IAPS groups had lower serum malondialdehyde concentration and higher serum total antioxidant capacity activity and mucosal interleukin 2 mRNA expression of jejunum than those in the control group (P < 0.05). In addition, birds in the control groups had lower duodenal and jejunal IgA-producing cells number than these in other 4 groups (P < 0.05). As compared with the CTC group, dietary individual XOS or IAPS administration increased duodenal IgA-producing cells number (P < 0.05). Meanwhile, there was an interaction between XOS and IAPS treatments on increasing duodenal and jejunal IgA-Producing cells numbers (P < 0.05). Dietary CTC administration increased the proportion of Bacteroides, and decreased the proportion of Negativibacillus (P < 0.05). However, dietary XOS + IAPS administration increased Firmicutes to Bacteroidetes ratio, the proportion of Ruminococcaceae, as well as decreased the proportion of Barnesiella and Negativibacillus (P < 0.05). In conclusion, the XOS and IAPS combination could improve intestinal mucosal immunity and barrier function of broilers by enhancing cytokine gene expression, IgA-producing cell production and modulates cecal microbiota, and the combination effect of XOS and IAPS is better than that of individual effect of CTC, XOS, or IAPS in the current study. |
| first_indexed | 2024-04-11T21:21:04Z |
| format | Article |
| id | doaj.art-96c5cba570fa439997c87dccbe53f2b9 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-11T21:21:04Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-96c5cba570fa439997c87dccbe53f2b92022-12-22T04:02:37ZengElsevierPoultry Science0032-57912022-09-011019101996The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilersQ. Wang0X.F. Wang1T. Xing2J.L. Li3X.D. Zhu4L. Zhang5F. Gao6College of Animal Science and Technology, Jiangsu Provincial Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Provincial Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Science, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Jiangsu Provincial Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Provincial Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Jiangsu Provincial Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Provincial Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Science, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science and Technology, Jiangsu Provincial Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Provincial Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, China; Corresponding author:College of Animal Science and Technology, Jiangsu Provincial Key Laboratory of Animal Origin Food Production and Safety Guarantee, Jiangsu Provincial Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, ChinaABSTRACT: The present study investigated the individual and combined effects of xylo-oligosaccharides (XOS) and gamma-irradiated astragalus polysaccharides (IAPS) on the immune response, antioxidant capacity and intestinal microbiota composition of broiler chickens. A total of 240 newly hatched Ross 308 chicks were randomly allocated into 5 dietary treatments including the basal diet (control), or the basal diet supplemented with 50 mg/kg chlortetracycline (CTC), 100 mg/kg XOS (XOS), 600 mg/kg IAPS (IAPS), and 100 mg/kg XOS + 600 mg/kg IAPS (XOS + IAPS) respectively. The results showed that birds in the control group had lower the thymus index and serum lysozyme activity than those in the other 4 groups (P < 0.05). Moreover, there was an interaction between XOS and IAPS treatments on increasing the serum lysozyme activity (P < 0.05). Birds in the CTC and XOS + IAPS groups had lower serum malondialdehyde concentration and higher serum total antioxidant capacity activity and mucosal interleukin 2 mRNA expression of jejunum than those in the control group (P < 0.05). In addition, birds in the control groups had lower duodenal and jejunal IgA-producing cells number than these in other 4 groups (P < 0.05). As compared with the CTC group, dietary individual XOS or IAPS administration increased duodenal IgA-producing cells number (P < 0.05). Meanwhile, there was an interaction between XOS and IAPS treatments on increasing duodenal and jejunal IgA-Producing cells numbers (P < 0.05). Dietary CTC administration increased the proportion of Bacteroides, and decreased the proportion of Negativibacillus (P < 0.05). However, dietary XOS + IAPS administration increased Firmicutes to Bacteroidetes ratio, the proportion of Ruminococcaceae, as well as decreased the proportion of Barnesiella and Negativibacillus (P < 0.05). In conclusion, the XOS and IAPS combination could improve intestinal mucosal immunity and barrier function of broilers by enhancing cytokine gene expression, IgA-producing cell production and modulates cecal microbiota, and the combination effect of XOS and IAPS is better than that of individual effect of CTC, XOS, or IAPS in the current study.http://www.sciencedirect.com/science/article/pii/S0032579122002875broilerxylo-oligosaccharidesgamma-irradiated astragalus polysaccharidesimmune responsemicrobiota |
| spellingShingle | Q. Wang X.F. Wang T. Xing J.L. Li X.D. Zhu L. Zhang F. Gao The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers Poultry Science broiler xylo-oligosaccharides gamma-irradiated astragalus polysaccharides immune response microbiota |
| title | The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers |
| title_full | The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers |
| title_fullStr | The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers |
| title_full_unstemmed | The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers |
| title_short | The combined impact of xylo-oligosaccharides and gamma-irradiated astragalus polysaccharides on the immune response, antioxidant capacity, and intestinal microbiota composition of broilers |
| title_sort | combined impact of xylo oligosaccharides and gamma irradiated astragalus polysaccharides on the immune response antioxidant capacity and intestinal microbiota composition of broilers |
| topic | broiler xylo-oligosaccharides gamma-irradiated astragalus polysaccharides immune response microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122002875 |
| work_keys_str_mv | AT qwang thecombinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT xfwang thecombinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT txing thecombinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT jlli thecombinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT xdzhu thecombinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT lzhang thecombinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT fgao thecombinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT qwang combinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT xfwang combinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT txing combinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT jlli combinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT xdzhu combinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT lzhang combinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers AT fgao combinedimpactofxylooligosaccharidesandgammairradiatedastragaluspolysaccharidesontheimmuneresponseantioxidantcapacityandintestinalmicrobiotacompositionofbroilers |