Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens
According to the World Health Organization (WHO), around 1 million children worldwide are diagnosed with tuberculosis each year. The Bacillus Calmette–Guérin (BCG) vaccine has been used around the world for over 100 years. The complications of the BCG vaccination can occur in about 0,06% of children...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/12/12/1374 |
| _version_ | 1797379727085797376 |
|---|---|
| author | Sylwia Brzezińska Anna Zabost Dagmara Borkowska-Tatar Magdalena Klatt Jolanta Goździk Agnieszka Dłużniewska Katarzyna Błasińska Ewa Augustynowicz-Kopeć |
| author_facet | Sylwia Brzezińska Anna Zabost Dagmara Borkowska-Tatar Magdalena Klatt Jolanta Goździk Agnieszka Dłużniewska Katarzyna Błasińska Ewa Augustynowicz-Kopeć |
| author_sort | Sylwia Brzezińska |
| collection | DOAJ |
| description | According to the World Health Organization (WHO), around 1 million children worldwide are diagnosed with tuberculosis each year. The Bacillus Calmette–Guérin (BCG) vaccine has been used around the world for over 100 years. The complications of the BCG vaccination can occur in about 0,06% of children and include local or systemic adverse reactions. Due to the close analogy between the vaccine strain and other species of the <i>Mycobacterium tuberculosis</i> complex (MTBC), molecular methods are recommended for differential diagnosis of Vaccine adverse events (VAE) after BCG. The ability to quickly and specifically identify BCG is important in view of different treatment regimens. The aim of the study was to assess the usefulness of genetic testing for <i>Mycobacterium bovis</i> BCG in the paraffin-embedded specimens’ methods. We describe two cases of VAE in immune-compromised children presenting with osteoarticular changes that had been clinically suspected of tuberculosis and led to molecular identification through GeneXpert, GenoType MTBC, and Spoligotyping. Results: <i>Mycobacterium bovis</i> BCG was detected in osteoarticular changes embedded in paraffin block of two patients. Conclusion: Genetic tests using paraffin-embedded materials allow for quick identification and differential diagnosis of patients with Tuberculosis and VAE after BCG. This is an important issue, especially in cases where the tissue has only been submitted for histopathological examination without microbiological diagnostics for tuberculosis. |
| first_indexed | 2024-03-08T20:28:12Z |
| format | Article |
| id | doaj.art-96c90600904b44ddb2cc852b6bca818b |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-08T20:28:12Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-96c90600904b44ddb2cc852b6bca818b2023-12-22T14:30:44ZengMDPI AGPathogens2076-08172023-11-011212137410.3390/pathogens12121374Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded SpecimensSylwia Brzezińska0Anna Zabost1Dagmara Borkowska-Tatar2Magdalena Klatt3Jolanta Goździk4Agnieszka Dłużniewska5Katarzyna Błasińska6Ewa Augustynowicz-Kopeć7Department of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandDepartment of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandDepartment of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandDepartment of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandDepartment of Clinical Immunology and Transplantation, Jagiellonian University Medical College, 31-008 Krakow, PolandTransplantation Centre, University Children’s Hospital, 30-663 Krakow, PolandDepartment of Radiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandDepartment of Microbiology, National Tuberculosis and Lung Diseases Research Institute, 01-138 Warsaw, PolandAccording to the World Health Organization (WHO), around 1 million children worldwide are diagnosed with tuberculosis each year. The Bacillus Calmette–Guérin (BCG) vaccine has been used around the world for over 100 years. The complications of the BCG vaccination can occur in about 0,06% of children and include local or systemic adverse reactions. Due to the close analogy between the vaccine strain and other species of the <i>Mycobacterium tuberculosis</i> complex (MTBC), molecular methods are recommended for differential diagnosis of Vaccine adverse events (VAE) after BCG. The ability to quickly and specifically identify BCG is important in view of different treatment regimens. The aim of the study was to assess the usefulness of genetic testing for <i>Mycobacterium bovis</i> BCG in the paraffin-embedded specimens’ methods. We describe two cases of VAE in immune-compromised children presenting with osteoarticular changes that had been clinically suspected of tuberculosis and led to molecular identification through GeneXpert, GenoType MTBC, and Spoligotyping. Results: <i>Mycobacterium bovis</i> BCG was detected in osteoarticular changes embedded in paraffin block of two patients. Conclusion: Genetic tests using paraffin-embedded materials allow for quick identification and differential diagnosis of patients with Tuberculosis and VAE after BCG. This is an important issue, especially in cases where the tissue has only been submitted for histopathological examination without microbiological diagnostics for tuberculosis.https://www.mdpi.com/2076-0817/12/12/1374<i>Mycobacterium bovis</i> BCGgenetic testgranulomashistopathological specimens |
| spellingShingle | Sylwia Brzezińska Anna Zabost Dagmara Borkowska-Tatar Magdalena Klatt Jolanta Goździk Agnieszka Dłużniewska Katarzyna Błasińska Ewa Augustynowicz-Kopeć Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens Pathogens <i>Mycobacterium bovis</i> BCG genetic test granulomas histopathological specimens |
| title | Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens |
| title_full | Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens |
| title_fullStr | Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens |
| title_full_unstemmed | Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens |
| title_short | Molecular Identification of Extrapulmonary Vaccine Adverse Events after BCG in Paraffin-Embedded Specimens |
| title_sort | molecular identification of extrapulmonary vaccine adverse events after bcg in paraffin embedded specimens |
| topic | <i>Mycobacterium bovis</i> BCG genetic test granulomas histopathological specimens |
| url | https://www.mdpi.com/2076-0817/12/12/1374 |
| work_keys_str_mv | AT sylwiabrzezinska molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens AT annazabost molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens AT dagmaraborkowskatatar molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens AT magdalenaklatt molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens AT jolantagozdzik molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens AT agnieszkadłuzniewska molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens AT katarzynabłasinska molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens AT ewaaugustynowiczkopec molecularidentificationofextrapulmonaryvaccineadverseeventsafterbcginparaffinembeddedspecimens |