Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the Mouse
Abstract.: Renal ischemia produces renal sympathoexcitation that is responsible for the development of ischemic acute kidney injury. The present study examined changes in the sympathetic nerve function in mice. Ischemic acute kidney injury was induced by occlusion of both renal pedicles. Renal ische...
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319301112 |
| _version_ | 1818776882597855232 |
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| author | Junpei Mutoh Masahiro Ohsawa Hiroaki Hisa |
| author_facet | Junpei Mutoh Masahiro Ohsawa Hiroaki Hisa |
| author_sort | Junpei Mutoh |
| collection | DOAJ |
| description | Abstract.: Renal ischemia produces renal sympathoexcitation that is responsible for the development of ischemic acute kidney injury. The present study examined changes in the sympathetic nerve function in mice. Ischemic acute kidney injury was induced by occlusion of both renal pedicles. Renal ischemia/reperfusion increased blood urea nitrogen and plasma creatinine and expression of tyrosine hydroxylase, a rate-limiting enzyme for the biosynthesis of noradrenaline, in the kidney. Renal immunoreactivity of tyrosine hydroxylase was observed along with vessel and tubular structure both in the sham-operated and the ischemic acute kidney injury mice. The prominent morphological change was that tyrosine hydroxylase immunoreactivity was observed in the glomeruli of the ischemic acute kidney injury mice, whereas there are almost no tyrosine hydroxylase immunoreactivity signals in the glomeruli of the sham-operated mice. This tyrosine hydroxylase immunoreactivity in the glomeruli is colocalized with synapsin I immunoreactivity in the ischemic acute kidney injury mice. Intraperitoneal pretreatment with DSP-4 (50 mg/kg) attenuated these changes induced by renal ischemia/reperfusion. These results suggest that morphological and functional changes of glomerulus adrenergic nerve terminal are involved in the pathophysiology of ischemia/reperfusion-induced ischemic acute kidney injury. Keywords:: acute kidney injury, adrenergic neuron, tyrosine hydroxylase |
| first_indexed | 2024-12-18T11:20:00Z |
| format | Article |
| id | doaj.art-96c94bae72044e7f81095b255b4ac417 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-18T11:20:00Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-96c94bae72044e7f81095b255b4ac4172022-12-21T21:09:50ZengElsevierJournal of Pharmacological Sciences1347-86132014-01-011254415421Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the MouseJunpei Mutoh0Masahiro Ohsawa1Hiroaki Hisa2Second Department of Pharmacology, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-Machi, Nobeoka-Shi, Miyazaki 882-8508, JapanSecond Department of Pharmacology, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-Machi, Nobeoka-Shi, Miyazaki 882-8508, JapanSecond Department of Pharmacology, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshino-Machi, Nobeoka-Shi, Miyazaki 882-8508, Japan; Corresponding author. hhisa@phoenix.ac.jpAbstract.: Renal ischemia produces renal sympathoexcitation that is responsible for the development of ischemic acute kidney injury. The present study examined changes in the sympathetic nerve function in mice. Ischemic acute kidney injury was induced by occlusion of both renal pedicles. Renal ischemia/reperfusion increased blood urea nitrogen and plasma creatinine and expression of tyrosine hydroxylase, a rate-limiting enzyme for the biosynthesis of noradrenaline, in the kidney. Renal immunoreactivity of tyrosine hydroxylase was observed along with vessel and tubular structure both in the sham-operated and the ischemic acute kidney injury mice. The prominent morphological change was that tyrosine hydroxylase immunoreactivity was observed in the glomeruli of the ischemic acute kidney injury mice, whereas there are almost no tyrosine hydroxylase immunoreactivity signals in the glomeruli of the sham-operated mice. This tyrosine hydroxylase immunoreactivity in the glomeruli is colocalized with synapsin I immunoreactivity in the ischemic acute kidney injury mice. Intraperitoneal pretreatment with DSP-4 (50 mg/kg) attenuated these changes induced by renal ischemia/reperfusion. These results suggest that morphological and functional changes of glomerulus adrenergic nerve terminal are involved in the pathophysiology of ischemia/reperfusion-induced ischemic acute kidney injury. Keywords:: acute kidney injury, adrenergic neuron, tyrosine hydroxylasehttp://www.sciencedirect.com/science/article/pii/S1347861319301112 |
| spellingShingle | Junpei Mutoh Masahiro Ohsawa Hiroaki Hisa Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the Mouse Journal of Pharmacological Sciences |
| title | Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the Mouse |
| title_full | Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the Mouse |
| title_fullStr | Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the Mouse |
| title_full_unstemmed | Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the Mouse |
| title_short | Involvement of Renal Sympathetic Nerve Activation on the Progression of Ischemic Acute Kidney Injury in the Mouse |
| title_sort | involvement of renal sympathetic nerve activation on the progression of ischemic acute kidney injury in the mouse |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319301112 |
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