First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa
QUESTION UNDER STUDY: The feasibility and the potential advantages of separating X-chromosome bearing spermatozoa for the prevention of a severe X-chromosome linked disorder with the use of intracytoplasmic sperm injection are presented. METHOD: A carrier of muscular dystrophy type Be...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SMW supporting association (Trägerverein Swiss Medical Weekly SMW)
2013-02-01
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| Series: | Swiss Medical Weekly |
| Subjects: | |
| Online Access: | https://www.smw.ch/index.php/smw/article/view/1647 |
| _version_ | 1811289176746754048 |
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| author | Christian De Geyter Oliver Sterthaus Peter Miny Friedel Wenzel Olav Lapaire Maria De Geyter Gideon Sartorius |
| author_facet | Christian De Geyter Oliver Sterthaus Peter Miny Friedel Wenzel Olav Lapaire Maria De Geyter Gideon Sartorius |
| author_sort | Christian De Geyter |
| collection | DOAJ |
| description |
QUESTION UNDER STUDY: The feasibility and the potential advantages of separating X-chromosome bearing spermatozoa for the prevention of a severe X-chromosome linked disorder with the use of intracytoplasmic sperm injection are presented.
METHOD: A carrier of muscular dystrophy type Becker was treated with intracytoplasmic sperm injection, using spermatozoa previously stained with the Hoechst dye 33342 and sorted with flow cytometry.
RESULTS: After transfer of one single blastocyst, an intrauterine pregnancy arose. In the ninth week of gestation, the female sex of the embryo was confirmed with proof of absence of the SRY gene of the Y-chromosome. After normal pregnancy, the patient delivered a healthy daughter.
CONCLUSIONS: The staining of spermatozoa with specific markers and sorting with flow cytometry provides a means of preventing significant disease in the offspring and may help in reducing the number of surplus embryos needed for preimplantation genetic diagnosis.
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| first_indexed | 2024-04-13T03:50:16Z |
| format | Article |
| id | doaj.art-96c9bfdc8ef34d259b40f1302a971472 |
| institution | Directory Open Access Journal |
| issn | 1424-3997 |
| language | English |
| last_indexed | 2024-04-13T03:50:16Z |
| publishDate | 2013-02-01 |
| publisher | SMW supporting association (Trägerverein Swiss Medical Weekly SMW) |
| record_format | Article |
| series | Swiss Medical Weekly |
| spelling | doaj.art-96c9bfdc8ef34d259b40f1302a9714722022-12-22T03:03:51ZengSMW supporting association (Trägerverein Swiss Medical Weekly SMW)Swiss Medical Weekly1424-39972013-02-01143070810.4414/smw.2013.13718First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoaChristian De GeyterOliver SterthausPeter MinyFriedel WenzelOlav LapaireMaria De GeyterGideon Sartorius QUESTION UNDER STUDY: The feasibility and the potential advantages of separating X-chromosome bearing spermatozoa for the prevention of a severe X-chromosome linked disorder with the use of intracytoplasmic sperm injection are presented. METHOD: A carrier of muscular dystrophy type Becker was treated with intracytoplasmic sperm injection, using spermatozoa previously stained with the Hoechst dye 33342 and sorted with flow cytometry. RESULTS: After transfer of one single blastocyst, an intrauterine pregnancy arose. In the ninth week of gestation, the female sex of the embryo was confirmed with proof of absence of the SRY gene of the Y-chromosome. After normal pregnancy, the patient delivered a healthy daughter. CONCLUSIONS: The staining of spermatozoa with specific markers and sorting with flow cytometry provides a means of preventing significant disease in the offspring and may help in reducing the number of surplus embryos needed for preimplantation genetic diagnosis. https://www.smw.ch/index.php/smw/article/view/1647assisted reproductionflow cytometrypreimplantation genetic diagnosissemen preparationX-linked disorders |
| spellingShingle | Christian De Geyter Oliver Sterthaus Peter Miny Friedel Wenzel Olav Lapaire Maria De Geyter Gideon Sartorius First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa Swiss Medical Weekly assisted reproduction flow cytometry preimplantation genetic diagnosis semen preparation X-linked disorders |
| title | First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa |
| title_full | First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa |
| title_fullStr | First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa |
| title_full_unstemmed | First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa |
| title_short | First successful pregnancy in Switzerland after prospective sex determination of the embryo through the separation of X-chromosome bearing spermatozoa |
| title_sort | first successful pregnancy in switzerland after prospective sex determination of the embryo through the separation of x chromosome bearing spermatozoa |
| topic | assisted reproduction flow cytometry preimplantation genetic diagnosis semen preparation X-linked disorders |
| url | https://www.smw.ch/index.php/smw/article/view/1647 |
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