Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 Enterotype
Background: Dietary intervention is a cornerstone of weight loss therapies. In obesity, a dysbiotic gut microbiota (GM) is characterized by high levels of Bacteroides lineages and low diversity. We examined the GM composition changes, including the Bacteroides 2 enterotype (Bact2), in a real-world w...
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MDPI AG
2021-12-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/1/16 |
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| author | Rohia Alili Eugeni Belda Odile Fabre Véronique Pelloux Nils Giordano Rémy Legrand Pierre Bel Lassen Timothy D. Swartz Jean-Daniel Zucker Karine Clément |
| author_facet | Rohia Alili Eugeni Belda Odile Fabre Véronique Pelloux Nils Giordano Rémy Legrand Pierre Bel Lassen Timothy D. Swartz Jean-Daniel Zucker Karine Clément |
| author_sort | Rohia Alili |
| collection | DOAJ |
| description | Background: Dietary intervention is a cornerstone of weight loss therapies. In obesity, a dysbiotic gut microbiota (GM) is characterized by high levels of Bacteroides lineages and low diversity. We examined the GM composition changes, including the Bacteroides 2 enterotype (Bact2), in a real-world weight loss study in subjects following a high-protein hypocaloric diet with or without a live microorganisms (LMP) supplement. Method: 263 volunteers were part of this real-world weight loss program. The first phase was a high-protein low-carbohydrate calorie restriction diet with or without LMP supplements. Fecal samples were obtained at baseline and after 10% weight loss for 163 subjects. Metagenomic profiling was obtained by shotgun sequencing. Results: At baseline, the Bact2 enterotype was more prevalent in subjects with aggravated obesity and metabolic alterations. After weight loss, diversity increased and Bact2 prevalence decreased in subjects with lower GM diversity at baseline, notably in LMP consumers. Significant increases in <i>Akkermansia muciniphila</i> and <i>Parabacteroides distasonis</i> and significant decreases of <i>Eubacterium rectale</i>, <i>Streptococcus thermophilus</i> and Bifidobacterial lineages were observed after weight loss. Conclusions: Baseline microbiome composition is associated with differential changes in GM diversity and Bact2 enterotype prevalence after weight loss. Examining these signatures could drive future personalized nutrition efforts towards more favorable microbiome compositions. |
| first_indexed | 2024-03-10T01:53:08Z |
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| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T01:53:08Z |
| publishDate | 2021-12-01 |
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| series | Biomedicines |
| spelling | doaj.art-96c9e454d63547de89781e58f83d39c82023-11-23T13:02:21ZengMDPI AGBiomedicines2227-90592021-12-011011610.3390/biomedicines10010016Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 EnterotypeRohia Alili0Eugeni Belda1Odile Fabre2Véronique Pelloux3Nils Giordano4Rémy Legrand5Pierre Bel Lassen6Timothy D. Swartz7Jean-Daniel Zucker8Karine Clément9Nutrition and Obesities, Systemic Approaches, NutriOmics Research Unit, INSERM, Sorbonne Université, 75013 Paris, FranceIntegrative Phenomics, 75011 Paris, FranceGroupe Éthique et Santé, 13400 Aubagne, FranceNutrition and Obesities, Systemic Approaches, NutriOmics Research Unit, INSERM, Sorbonne Université, 75013 Paris, FranceIntegrative Phenomics, 75011 Paris, FranceGroupe Éthique et Santé, 13400 Aubagne, FranceNutrition and Obesities, Systemic Approaches, NutriOmics Research Unit, INSERM, Sorbonne Université, 75013 Paris, FranceIntegrative Phenomics, 75011 Paris, FranceNutrition and Obesities, Systemic Approaches, NutriOmics Research Unit, INSERM, Sorbonne Université, 75013 Paris, FranceNutrition and Obesities, Systemic Approaches, NutriOmics Research Unit, INSERM, Sorbonne Université, 75013 Paris, FranceBackground: Dietary intervention is a cornerstone of weight loss therapies. In obesity, a dysbiotic gut microbiota (GM) is characterized by high levels of Bacteroides lineages and low diversity. We examined the GM composition changes, including the Bacteroides 2 enterotype (Bact2), in a real-world weight loss study in subjects following a high-protein hypocaloric diet with or without a live microorganisms (LMP) supplement. Method: 263 volunteers were part of this real-world weight loss program. The first phase was a high-protein low-carbohydrate calorie restriction diet with or without LMP supplements. Fecal samples were obtained at baseline and after 10% weight loss for 163 subjects. Metagenomic profiling was obtained by shotgun sequencing. Results: At baseline, the Bact2 enterotype was more prevalent in subjects with aggravated obesity and metabolic alterations. After weight loss, diversity increased and Bact2 prevalence decreased in subjects with lower GM diversity at baseline, notably in LMP consumers. Significant increases in <i>Akkermansia muciniphila</i> and <i>Parabacteroides distasonis</i> and significant decreases of <i>Eubacterium rectale</i>, <i>Streptococcus thermophilus</i> and Bifidobacterial lineages were observed after weight loss. Conclusions: Baseline microbiome composition is associated with differential changes in GM diversity and Bact2 enterotype prevalence after weight loss. Examining these signatures could drive future personalized nutrition efforts towards more favorable microbiome compositions.https://www.mdpi.com/2227-9059/10/1/16obesitygut microbiotaweight lossBacteroides 2 enterotypereal-world dietary interventionnanopore technology |
| spellingShingle | Rohia Alili Eugeni Belda Odile Fabre Véronique Pelloux Nils Giordano Rémy Legrand Pierre Bel Lassen Timothy D. Swartz Jean-Daniel Zucker Karine Clément Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 Enterotype Biomedicines obesity gut microbiota weight loss Bacteroides 2 enterotype real-world dietary intervention nanopore technology |
| title | Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 Enterotype |
| title_full | Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 Enterotype |
| title_fullStr | Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 Enterotype |
| title_full_unstemmed | Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 Enterotype |
| title_short | Characterization of the Gut Microbiota in Individuals with Overweight or Obesity during a Real-World Weight Loss Dietary Program: A Focus on the Bacteroides 2 Enterotype |
| title_sort | characterization of the gut microbiota in individuals with overweight or obesity during a real world weight loss dietary program a focus on the bacteroides 2 enterotype |
| topic | obesity gut microbiota weight loss Bacteroides 2 enterotype real-world dietary intervention nanopore technology |
| url | https://www.mdpi.com/2227-9059/10/1/16 |
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