<i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications
<i>Acheta domesticus</i> is an edible insect, rich in nutritional value and considered a sustainable protein source. This study aimed to investigate the potential application of <i>A. domesticus</i> extracts for anti-skin-aging purposes. The extracts were prepared by macerati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/17/3/346 |
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| author | Kankanit Yeerong Panuwan Chantawannakul Songyot Anuchapreeda Thomas Rades Anette Müllertz Wantida Chaiyana |
| author_facet | Kankanit Yeerong Panuwan Chantawannakul Songyot Anuchapreeda Thomas Rades Anette Müllertz Wantida Chaiyana |
| author_sort | Kankanit Yeerong |
| collection | DOAJ |
| description | <i>Acheta domesticus</i> is an edible insect, rich in nutritional value and considered a sustainable protein source. This study aimed to investigate the potential application of <i>A. domesticus</i> extracts for anti-skin-aging purposes. The extracts were prepared by maceration at ambient temperature with 95% ethanol or hexane and maceration in gentle heat (45 °C) with 95% <i>v</i>/<i>v</i> ethanol or DI water. The extracts were examined for total protein, phenolic, and flavonoid contents. Protein molecular weight distribution was analyzed. The safety of the extracts was investigated in terms of irritation and cytotoxicity. Biological activities relevant to the inhibition of skin aging were evaluated, including increasing transforming growth factor-beta 1 (TGF-β1) expression and inhibitory activities on collagenase and hyaluronidase. The aqueous extract from maceration in gentle heat had the highest total protein content (63 ± 1% <i>w</i>/<i>w</i>), total phenolic content (0.48 ± 0.03 mg GAE/g extract), TGF-β1 stimulating activities (33 ± 2 pg/mL), and collagenase inhibition (with a half maximal inhibitory concentration of 26 ± 1 µg/mL) among various extracts investigated. It caused no irritation to the hen’s egg chorioallantoic membrane and showed no cytotoxicity to human dermal fibroblasts and peripheral blood mononuclear cells. Therefore, aqueous <i>A. domesticus</i> extract is proposed as an innovative natural anti-skin-aging ingredient. |
| first_indexed | 2024-04-24T17:56:15Z |
| format | Article |
| id | doaj.art-96ce4c762c55472e9746a3799dc8ca89 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-04-24T17:56:15Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-96ce4c762c55472e9746a3799dc8ca892024-03-27T13:59:21ZengMDPI AGPharmaceuticals1424-82472024-03-0117334610.3390/ph17030346<i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic ApplicationsKankanit Yeerong0Panuwan Chantawannakul1Songyot Anuchapreeda2Thomas Rades3Anette Müllertz4Wantida Chaiyana5Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, ThailandBee Protection Laboratory, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, DenmarkDepartment of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, DenmarkDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand<i>Acheta domesticus</i> is an edible insect, rich in nutritional value and considered a sustainable protein source. This study aimed to investigate the potential application of <i>A. domesticus</i> extracts for anti-skin-aging purposes. The extracts were prepared by maceration at ambient temperature with 95% ethanol or hexane and maceration in gentle heat (45 °C) with 95% <i>v</i>/<i>v</i> ethanol or DI water. The extracts were examined for total protein, phenolic, and flavonoid contents. Protein molecular weight distribution was analyzed. The safety of the extracts was investigated in terms of irritation and cytotoxicity. Biological activities relevant to the inhibition of skin aging were evaluated, including increasing transforming growth factor-beta 1 (TGF-β1) expression and inhibitory activities on collagenase and hyaluronidase. The aqueous extract from maceration in gentle heat had the highest total protein content (63 ± 1% <i>w</i>/<i>w</i>), total phenolic content (0.48 ± 0.03 mg GAE/g extract), TGF-β1 stimulating activities (33 ± 2 pg/mL), and collagenase inhibition (with a half maximal inhibitory concentration of 26 ± 1 µg/mL) among various extracts investigated. It caused no irritation to the hen’s egg chorioallantoic membrane and showed no cytotoxicity to human dermal fibroblasts and peripheral blood mononuclear cells. Therefore, aqueous <i>A. domesticus</i> extract is proposed as an innovative natural anti-skin-aging ingredient.https://www.mdpi.com/1424-8247/17/3/346<i>Acheta domesticus</i>anti-aging skincarecosmetichuman dermal fibroblastsafety |
| spellingShingle | Kankanit Yeerong Panuwan Chantawannakul Songyot Anuchapreeda Thomas Rades Anette Müllertz Wantida Chaiyana <i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications Pharmaceuticals <i>Acheta domesticus</i> anti-aging skincare cosmetic human dermal fibroblast safety |
| title | <i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications |
| title_full | <i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications |
| title_fullStr | <i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications |
| title_full_unstemmed | <i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications |
| title_short | <i>Acheta domesticus</i>: A Natural Source of Anti-Skin-Aging Ingredients for Cosmetic Applications |
| title_sort | i acheta domesticus i a natural source of anti skin aging ingredients for cosmetic applications |
| topic | <i>Acheta domesticus</i> anti-aging skincare cosmetic human dermal fibroblast safety |
| url | https://www.mdpi.com/1424-8247/17/3/346 |
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