Established Liver Cell Lines: Are You Sure to Have the Right Ones?
In liver research, immortalized cell lines have assumed an important role in studying general physiological and pathological processes. However, misidentification and cross-contamination of cell lines is a widespread problem in biomedical sciences resulting in irreproducible results and false conclu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Livers |
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| Online Access: | https://www.mdpi.com/2673-4389/2/3/15 |
| _version_ | 1827659450968702976 |
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| author | Ralf Weiskirchen |
| author_facet | Ralf Weiskirchen |
| author_sort | Ralf Weiskirchen |
| collection | DOAJ |
| description | In liver research, immortalized cell lines have assumed an important role in studying general physiological and pathological processes. However, misidentification and cross-contamination of cell lines is a widespread problem in biomedical sciences resulting in irreproducible results and false conclusions. Although the huge impact of working with wrong cell lines on life science research and publication has been well recognized, there are only limited efforts and strategies to prevent cell misidentification. This commentary provides a catalogue of the most important cell lines used in hepatology research, examples of misidentified cell lines, and short guidelines to be considered when working with continuous lines. |
| first_indexed | 2024-03-09T23:22:38Z |
| format | Article |
| id | doaj.art-96d0748fbf0b42e982a9e0f75a6b7a4e |
| institution | Directory Open Access Journal |
| issn | 2673-4389 |
| language | English |
| last_indexed | 2024-03-09T23:22:38Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Livers |
| spelling | doaj.art-96d0748fbf0b42e982a9e0f75a6b7a4e2023-11-23T17:24:36ZengMDPI AGLivers2673-43892022-09-012317117710.3390/livers2030015Established Liver Cell Lines: Are You Sure to Have the Right Ones?Ralf Weiskirchen0Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, GermanyIn liver research, immortalized cell lines have assumed an important role in studying general physiological and pathological processes. However, misidentification and cross-contamination of cell lines is a widespread problem in biomedical sciences resulting in irreproducible results and false conclusions. Although the huge impact of working with wrong cell lines on life science research and publication has been well recognized, there are only limited efforts and strategies to prevent cell misidentification. This commentary provides a catalogue of the most important cell lines used in hepatology research, examples of misidentified cell lines, and short guidelines to be considered when working with continuous lines.https://www.mdpi.com/2673-4389/2/3/15cell line misidentificationcross-contaminationSTR profilingcapillary electrophoresistranslational researchcell banking |
| spellingShingle | Ralf Weiskirchen Established Liver Cell Lines: Are You Sure to Have the Right Ones? Livers cell line misidentification cross-contamination STR profiling capillary electrophoresis translational research cell banking |
| title | Established Liver Cell Lines: Are You Sure to Have the Right Ones? |
| title_full | Established Liver Cell Lines: Are You Sure to Have the Right Ones? |
| title_fullStr | Established Liver Cell Lines: Are You Sure to Have the Right Ones? |
| title_full_unstemmed | Established Liver Cell Lines: Are You Sure to Have the Right Ones? |
| title_short | Established Liver Cell Lines: Are You Sure to Have the Right Ones? |
| title_sort | established liver cell lines are you sure to have the right ones |
| topic | cell line misidentification cross-contamination STR profiling capillary electrophoresis translational research cell banking |
| url | https://www.mdpi.com/2673-4389/2/3/15 |
| work_keys_str_mv | AT ralfweiskirchen establishedlivercelllinesareyousuretohavetherightones |