A multi-well bioreactor for cartilage tissue engineering experiments
Summary: Cartilage tissue engineering necessitates the right mechanical cues to regenerate impaired tissue. For this reason, bioreactors can be employed to induce joint-relevant mechanical loading, such as compression and shear. However, current articulating joint bioreactor designs are lacking in t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223011690 |
| _version_ | 1827895506564546560 |
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| author | Yann D. Ladner Hermann Kasper Angela R. Armiento Martin J. Stoddart |
| author_facet | Yann D. Ladner Hermann Kasper Angela R. Armiento Martin J. Stoddart |
| author_sort | Yann D. Ladner |
| collection | DOAJ |
| description | Summary: Cartilage tissue engineering necessitates the right mechanical cues to regenerate impaired tissue. For this reason, bioreactors can be employed to induce joint-relevant mechanical loading, such as compression and shear. However, current articulating joint bioreactor designs are lacking in terms of sample size and usability.In this paper, we describe a new, simple-to-build and operate, multi-well kinematic load bioreactor and investigate its effect on the chondrogenic differentiation of human bone marrow-derived stem cells (MSCs). We seeded MSCs into a fibrin-polyurethane scaffold and subsequently exposed the samples to a combination of compression and shear for 25 days. The mechanical loading activates transforming growth factor beta 1, upregulates chondrogenic genes, and increases sulfated glycosaminoglycan retention within the scaffolds.Such a higher-throughput bioreactor could be operated in most cell culture laboratories, dramatically accelerating and improving the testing of cells, new biomaterials, and tissue-engineered constructs. |
| first_indexed | 2024-03-12T22:22:51Z |
| format | Article |
| id | doaj.art-96d2471a4f7e451aada0caf0a8d33d30 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:22:51Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-96d2471a4f7e451aada0caf0a8d33d302023-07-23T04:55:30ZengElsevieriScience2589-00422023-07-01267107092A multi-well bioreactor for cartilage tissue engineering experimentsYann D. Ladner0Hermann Kasper1Angela R. Armiento2Martin J. Stoddart3AO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland; Institute for Biomechanics, ETH Zurich, Lengghalde 5, CH-8008 Zurich, SwitzerlandAO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, SwitzerlandAO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland; UCB Pharma, Slough, UKAO Research Institute Davos, Clavadelerstrasse 8, 7270 Davos Platz, Switzerland; Department of Orthopedics and Trauma Surgery, Medical Center-Albert-Ludwigs-University of Freiburg, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, 79106 Freiburg, Germany; Corresponding authorSummary: Cartilage tissue engineering necessitates the right mechanical cues to regenerate impaired tissue. For this reason, bioreactors can be employed to induce joint-relevant mechanical loading, such as compression and shear. However, current articulating joint bioreactor designs are lacking in terms of sample size and usability.In this paper, we describe a new, simple-to-build and operate, multi-well kinematic load bioreactor and investigate its effect on the chondrogenic differentiation of human bone marrow-derived stem cells (MSCs). We seeded MSCs into a fibrin-polyurethane scaffold and subsequently exposed the samples to a combination of compression and shear for 25 days. The mechanical loading activates transforming growth factor beta 1, upregulates chondrogenic genes, and increases sulfated glycosaminoglycan retention within the scaffolds.Such a higher-throughput bioreactor could be operated in most cell culture laboratories, dramatically accelerating and improving the testing of cells, new biomaterials, and tissue-engineered constructs.http://www.sciencedirect.com/science/article/pii/S2589004223011690BiotechnologyTissue EngineeringCell biologyBioengineering |
| spellingShingle | Yann D. Ladner Hermann Kasper Angela R. Armiento Martin J. Stoddart A multi-well bioreactor for cartilage tissue engineering experiments iScience Biotechnology Tissue Engineering Cell biology Bioengineering |
| title | A multi-well bioreactor for cartilage tissue engineering experiments |
| title_full | A multi-well bioreactor for cartilage tissue engineering experiments |
| title_fullStr | A multi-well bioreactor for cartilage tissue engineering experiments |
| title_full_unstemmed | A multi-well bioreactor for cartilage tissue engineering experiments |
| title_short | A multi-well bioreactor for cartilage tissue engineering experiments |
| title_sort | multi well bioreactor for cartilage tissue engineering experiments |
| topic | Biotechnology Tissue Engineering Cell biology Bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223011690 |
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