Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances
Despite process similarities, distinctive manufacturing technologies offer hyaluronic acid dermal fillers with different in vitro physicochemical and rheological properties due to peculiar crosslinked hydrogel networks. A better understanding of dermal filler properties could provide specific clinic...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/9/733 |
| _version_ | 1797579963439775744 |
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| author | Stefano Pluda Cecilia Salvagnini Anna Fontana Anna Marchetti Alba Di Lucia Devis Galesso Cristian Guarise |
| author_facet | Stefano Pluda Cecilia Salvagnini Anna Fontana Anna Marchetti Alba Di Lucia Devis Galesso Cristian Guarise |
| author_sort | Stefano Pluda |
| collection | DOAJ |
| description | Despite process similarities, distinctive manufacturing technologies offer hyaluronic acid dermal fillers with different in vitro physicochemical and rheological properties due to peculiar crosslinked hydrogel networks. A better understanding of dermal filler properties could provide specific clinical indications and expectations with more accurate performance correlations. In this study, with an emphasis on the degree of modification, hyaluronic acid concentration and molecular weight, these process parameters were able to modulate dermal filler properties, especially rheology. Moreover, an extensive characterization of commercial hyaluronic acid injectables of the Hyal System line was described to present product properties and help to elucidate related clinical effects. Standardized methodologies were applied to correlate in vitro parameters with feasible clinical indications. In view of an optimized dermal filler design, the results of the extrudability measurements allowed the quantification of the effect of hydrogel composition, rheological properties and needle size on injectability. Composition, dynamic viscosity and needle size showed an impactful influence on hydrogel extrudability. Finally, the positive influence of 200 KDa hyaluronic acid in comparison to fragments of ether-crosslinked hyaluronic acid on fibroblast recognition were shown with a migration assay. |
| first_indexed | 2024-03-10T22:43:35Z |
| format | Article |
| id | doaj.art-96d4214b84cc439ba4c7150d967b780a |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T22:43:35Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-96d4214b84cc439ba4c7150d967b780a2023-11-19T10:51:04ZengMDPI AGGels2310-28612023-09-019973310.3390/gels9090733Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product PerformancesStefano Pluda0Cecilia Salvagnini1Anna Fontana2Anna Marchetti3Alba Di Lucia4Devis Galesso5Cristian Guarise6Fidia Farmaceutici S.p.A., via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031 Padova, ItalyDepartment of Biology, University of Padova, 35121 Padova, ItalyDepartment of Biology, University of Padova, 35121 Padova, ItalyFidia Farmaceutici S.p.A., via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031 Padova, ItalyFidia Farmaceutici S.p.A., via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031 Padova, ItalyFidia Farmaceutici S.p.A., via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031 Padova, ItalyFidia Farmaceutici S.p.A., via Ponte della Fabbrica 3/A, Abano Terme (PD), 35031 Padova, ItalyDespite process similarities, distinctive manufacturing technologies offer hyaluronic acid dermal fillers with different in vitro physicochemical and rheological properties due to peculiar crosslinked hydrogel networks. A better understanding of dermal filler properties could provide specific clinical indications and expectations with more accurate performance correlations. In this study, with an emphasis on the degree of modification, hyaluronic acid concentration and molecular weight, these process parameters were able to modulate dermal filler properties, especially rheology. Moreover, an extensive characterization of commercial hyaluronic acid injectables of the Hyal System line was described to present product properties and help to elucidate related clinical effects. Standardized methodologies were applied to correlate in vitro parameters with feasible clinical indications. In view of an optimized dermal filler design, the results of the extrudability measurements allowed the quantification of the effect of hydrogel composition, rheological properties and needle size on injectability. Composition, dynamic viscosity and needle size showed an impactful influence on hydrogel extrudability. Finally, the positive influence of 200 KDa hyaluronic acid in comparison to fragments of ether-crosslinked hyaluronic acid on fibroblast recognition were shown with a migration assay.https://www.mdpi.com/2310-2861/9/9/733crosslinkinghyaluronic aciddermal fillers |
| spellingShingle | Stefano Pluda Cecilia Salvagnini Anna Fontana Anna Marchetti Alba Di Lucia Devis Galesso Cristian Guarise Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances Gels crosslinking hyaluronic acid dermal fillers |
| title | Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances |
| title_full | Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances |
| title_fullStr | Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances |
| title_full_unstemmed | Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances |
| title_short | Investigation of Crosslinking Parameters and Characterization of Hyaluronic Acid Dermal Fillers: From Design to Product Performances |
| title_sort | investigation of crosslinking parameters and characterization of hyaluronic acid dermal fillers from design to product performances |
| topic | crosslinking hyaluronic acid dermal fillers |
| url | https://www.mdpi.com/2310-2861/9/9/733 |
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