The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016
Background: Prognostic significance of the programmed death-ligand-1 status in non-small cell lung carcinoma remains controversial. Aims: To show the programmed death-ligand-1 expression status in patients with non-small cell lung carcinoma and its effect on the prognosis and the relationship with...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2019-05-01
|
| Series: | Balkan Medical Journal |
| Subjects: | |
| Online Access: | http://balkanmedicaljournal.org/text.php?lang=en&id=2073 |
| _version_ | 1797909630651727872 |
|---|---|
| author | Songül Şahin Şebnem Batur Övgü Aydın Tülin Öztürk Akif Turna Büge Öz |
| author_facet | Songül Şahin Şebnem Batur Övgü Aydın Tülin Öztürk Akif Turna Büge Öz |
| author_sort | Songül Şahin |
| collection | DOAJ |
| description | Background: Prognostic significance of the programmed death-ligand-1 status in non-small cell lung carcinoma remains controversial.
Aims: To show the programmed death-ligand-1 expression status in patients with non-small cell lung carcinoma and its effect on the prognosis and the relationship with clinicopathologic data.
Study Design: Retrospective cross-sectional study.
Methods: The study included 208 cases who were diagnosed with NSCLC and who underwent surgical resection between 2001 and 2012. Programmed death-ligand-1 (SP142 clone) was applied to the histological sections acquired from the microarray paraffin blocks with immunohistochemistry. Staining intensity was scored as weak (+, 1), moderate (++, 2), and strong (+++, 3). Percentage (0%-100%) was multiplied by staining intensity (1-2-3) to calculate the H score. Four different cut-off values were used; 1: ≥1% (independent of intensity), 2: ≥5% (independent of intensity), 3: ≥5% moderate/strong staining (except for weak staining), 4: H score ≥30 values were considered positive. In this study, staining a single cell at any intensity was considered positive.
Results: Thirty-four out 208 cases (16.3%) had PDL-1 positive staining. PDL-1 expression was observed in patients with non-small cell lung carcinoma independent of the histological type or subtype (range; 0-25%). When the cut-off level was set to ≥5% with moderate and strong staining, the median overall survival was 45 months for the PD-L1 positive group and not reached for the PD-L1 negative group (p-value 0.024). PD-L1 positivity was significantly higher in patients over the age of 60 years and in cases with a tumor diameter of more than 5 cm (p=0.023 and 0.025, respectively).
Conclusion: PD-L1 expression is positive in 16.3% of patients with non-small cell lung cancer and may have a negative prognostic value. |
| first_indexed | 2024-04-10T11:11:22Z |
| format | Article |
| id | doaj.art-96da00e6b94545e9b10854290dac26c3 |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T11:11:22Z |
| publishDate | 2019-05-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-96da00e6b94545e9b10854290dac26c32023-02-15T16:19:07ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312019-05-0136318418910.4274/balkanmedj.galenos.2018.2018.0392The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016Songül Şahin0Şebnem Batur1Övgü Aydın2Tülin Öztürk3Akif Turna4Büge Öz5Pathology Laboratory, Çankırı State Hospital, Çankırı, TurkeyDepartment of Pathology, İstanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine, İstanbul, TurkeyDepartment of Pathology, İstanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine, İstanbul, TurkeyDepartment of Pathology, İstanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine, İstanbul, TurkeyDepartment of Thoracic Surgery, İstanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine, İstanbul, TurkeyDepartment of Pathology, İstanbul University-Cerrahpaşa, Cerrahpaşa School of Medicine, İstanbul, TurkeyBackground: Prognostic significance of the programmed death-ligand-1 status in non-small cell lung carcinoma remains controversial. Aims: To show the programmed death-ligand-1 expression status in patients with non-small cell lung carcinoma and its effect on the prognosis and the relationship with clinicopathologic data. Study Design: Retrospective cross-sectional study. Methods: The study included 208 cases who were diagnosed with NSCLC and who underwent surgical resection between 2001 and 2012. Programmed death-ligand-1 (SP142 clone) was applied to the histological sections acquired from the microarray paraffin blocks with immunohistochemistry. Staining intensity was scored as weak (+, 1), moderate (++, 2), and strong (+++, 3). Percentage (0%-100%) was multiplied by staining intensity (1-2-3) to calculate the H score. Four different cut-off values were used; 1: ≥1% (independent of intensity), 2: ≥5% (independent of intensity), 3: ≥5% moderate/strong staining (except for weak staining), 4: H score ≥30 values were considered positive. In this study, staining a single cell at any intensity was considered positive. Results: Thirty-four out 208 cases (16.3%) had PDL-1 positive staining. PDL-1 expression was observed in patients with non-small cell lung carcinoma independent of the histological type or subtype (range; 0-25%). When the cut-off level was set to ≥5% with moderate and strong staining, the median overall survival was 45 months for the PD-L1 positive group and not reached for the PD-L1 negative group (p-value 0.024). PD-L1 positivity was significantly higher in patients over the age of 60 years and in cases with a tumor diameter of more than 5 cm (p=0.023 and 0.025, respectively). Conclusion: PD-L1 expression is positive in 16.3% of patients with non-small cell lung cancer and may have a negative prognostic value.http://balkanmedicaljournal.org/text.php?lang=en&id=2073Lung carcinomasnon-small-cellprogrammed death-ligand 1prognosis |
| spellingShingle | Songül Şahin Şebnem Batur Övgü Aydın Tülin Öztürk Akif Turna Büge Öz The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016 Balkan Medical Journal Lung carcinomas non-small-cell programmed death-ligand 1 prognosis |
| title | The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016 |
| title_full | The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016 |
| title_fullStr | The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016 |
| title_full_unstemmed | The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016 |
| title_short | The Incidence of Cystic Fibrosis in the Central Region of Anatolia in Turkey Between 2015 and 2016 |
| title_sort | incidence of cystic fibrosis in the central region of anatolia in turkey between 2015 and 2016 |
| topic | Lung carcinomas non-small-cell programmed death-ligand 1 prognosis |
| url | http://balkanmedicaljournal.org/text.php?lang=en&id=2073 |
| work_keys_str_mv | AT songulsahin theincidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT sebnembatur theincidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT ovguaydın theincidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT tulinozturk theincidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT akifturna theincidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT bugeoz theincidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT songulsahin incidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT sebnembatur incidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT ovguaydın incidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT tulinozturk incidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT akifturna incidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 AT bugeoz incidenceofcysticfibrosisinthecentralregionofanatoliainturkeybetween2015and2016 |