Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A Minireview
In recent years, there has been a growing understanding that a significant fraction of the eukaryotic proteome is intrinsically disordered, and that these conformationally dynamic proteins play a myriad of vital biological roles in both normal and pathological states. In this review, selected exampl...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/10/12/320 |
| _version_ | 1797546178933424128 |
|---|---|
| author | Frederik Lermyte |
| author_facet | Frederik Lermyte |
| author_sort | Frederik Lermyte |
| collection | DOAJ |
| description | In recent years, there has been a growing understanding that a significant fraction of the eukaryotic proteome is intrinsically disordered, and that these conformationally dynamic proteins play a myriad of vital biological roles in both normal and pathological states. In this review, selected examples of intrinsically disordered proteins are highlighted, with particular attention for a few which are relevant in neurological disorders and in viral infection. Next, the underlying causes for intrinsic disorder are discussed, along with computational methods used to predict whether a given amino acid sequence is likely to adopt a folded or unfolded state in solution. Finally, biophysical methods for the analysis of intrinsically disordered proteins will be discussed, as well as the unique challenges they pose in this context due to their highly dynamic nature. |
| first_indexed | 2024-03-10T14:25:25Z |
| format | Article |
| id | doaj.art-96da3a61207a440ebd267326ae1e5132 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T14:25:25Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-96da3a61207a440ebd267326ae1e51322023-11-20T23:01:05ZengMDPI AGLife2075-17292020-11-01101232010.3390/life10120320Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A MinireviewFrederik Lermyte0Department of Chemistry, Technical University of Darmstadt, Alarich-Weiss-Straße 4, 64287 Darmstadt, GermanyIn recent years, there has been a growing understanding that a significant fraction of the eukaryotic proteome is intrinsically disordered, and that these conformationally dynamic proteins play a myriad of vital biological roles in both normal and pathological states. In this review, selected examples of intrinsically disordered proteins are highlighted, with particular attention for a few which are relevant in neurological disorders and in viral infection. Next, the underlying causes for intrinsic disorder are discussed, along with computational methods used to predict whether a given amino acid sequence is likely to adopt a folded or unfolded state in solution. Finally, biophysical methods for the analysis of intrinsically disordered proteins will be discussed, as well as the unique challenges they pose in this context due to their highly dynamic nature.https://www.mdpi.com/2075-1729/10/12/320intrinsically disordered proteinstructural biologybiophysicsmass spectrometry |
| spellingShingle | Frederik Lermyte Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A Minireview Life intrinsically disordered protein structural biology biophysics mass spectrometry |
| title | Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A Minireview |
| title_full | Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A Minireview |
| title_fullStr | Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A Minireview |
| title_full_unstemmed | Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A Minireview |
| title_short | Roles, Characteristics, and Analysis of Intrinsically Disordered Proteins: A Minireview |
| title_sort | roles characteristics and analysis of intrinsically disordered proteins a minireview |
| topic | intrinsically disordered protein structural biology biophysics mass spectrometry |
| url | https://www.mdpi.com/2075-1729/10/12/320 |
| work_keys_str_mv | AT frederiklermyte rolescharacteristicsandanalysisofintrinsicallydisorderedproteinsaminireview |