In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i>
The aim of this study was to evaluate the antioxidant activities of extracts from olive leaves (EOL). The main contents of EOL were determined by colorimetric methods. The antioxidant activities were assessed by measuring the scavenging free radicals in vitro. To investigate the antioxidant activity...
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| Médium: | Článek |
| Jazyk: | English |
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MDPI AG
2019-02-01
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| Edice: | Molecules |
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| On-line přístup: | https://www.mdpi.com/1420-3049/24/4/704 |
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| author | Siyuan Luo Xuelian Jiang Liping Jia Chengyue Tan Min Li Qiuyu Yang Yanlin Du Chunbang Ding |
| author_facet | Siyuan Luo Xuelian Jiang Liping Jia Chengyue Tan Min Li Qiuyu Yang Yanlin Du Chunbang Ding |
| author_sort | Siyuan Luo |
| collection | DOAJ |
| description | The aim of this study was to evaluate the antioxidant activities of extracts from olive leaves (EOL). The main contents of EOL were determined by colorimetric methods. The antioxidant activities were assessed by measuring the scavenging free radicals in vitro. To investigate the antioxidant activity in vivo, we detected the survival of <i>Caenorhabditis elegans</i>, under thermal stress. Subsequently the reactive oxygen species (ROS) level, activities of antioxidant enzymes, the expression of HSP-16.2 and the translocation of <i>daf-16</i> were measured. The results showed that, polyphenols was the main component. EOL could well scavenge DPPH and superoxide anion radicals in vitro. Compared to the control group, the survival rate of <i>C. elegans</i> treated with EOL was extended by 10.43%, under heat stress. The ROS level was reduced, while the expression of <i>hsp-16.2</i> was increased to protect the organism against the increasing ROS. The level of malondialdehyde (MDA) also decreased sharply. The activities of inner antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were potentiated, which might have had a correlation with the DAF-16 transcription factor that was induced-turned into the nuclear. Therefore, EOL showed a strong antioxidant ability in vitro and in vivo. Hence, it could be a potential candidate when it came to medicinal and edible plants. |
| first_indexed | 2024-12-14T21:09:50Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-14T21:09:50Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-96db8e3463cf47f4a9a7490cfff90b512022-12-21T22:47:17ZengMDPI AGMolecules1420-30492019-02-0124470410.3390/molecules24040704molecules24040704In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i>Siyuan Luo0Xuelian Jiang1Liping Jia2Chengyue Tan3Min Li4Qiuyu Yang5Yanlin Du6Chunbang Ding7College of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625014, ChinaThe aim of this study was to evaluate the antioxidant activities of extracts from olive leaves (EOL). The main contents of EOL were determined by colorimetric methods. The antioxidant activities were assessed by measuring the scavenging free radicals in vitro. To investigate the antioxidant activity in vivo, we detected the survival of <i>Caenorhabditis elegans</i>, under thermal stress. Subsequently the reactive oxygen species (ROS) level, activities of antioxidant enzymes, the expression of HSP-16.2 and the translocation of <i>daf-16</i> were measured. The results showed that, polyphenols was the main component. EOL could well scavenge DPPH and superoxide anion radicals in vitro. Compared to the control group, the survival rate of <i>C. elegans</i> treated with EOL was extended by 10.43%, under heat stress. The ROS level was reduced, while the expression of <i>hsp-16.2</i> was increased to protect the organism against the increasing ROS. The level of malondialdehyde (MDA) also decreased sharply. The activities of inner antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were potentiated, which might have had a correlation with the DAF-16 transcription factor that was induced-turned into the nuclear. Therefore, EOL showed a strong antioxidant ability in vitro and in vivo. Hence, it could be a potential candidate when it came to medicinal and edible plants.https://www.mdpi.com/1420-3049/24/4/704olive leavespolyphenolsantioxidant activities<i>Caenorhabditis elegans</i>thermal stress |
| spellingShingle | Siyuan Luo Xuelian Jiang Liping Jia Chengyue Tan Min Li Qiuyu Yang Yanlin Du Chunbang Ding In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i> Molecules olive leaves polyphenols antioxidant activities <i>Caenorhabditis elegans</i> thermal stress |
| title | In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i> |
| title_full | In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i> |
| title_fullStr | In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i> |
| title_short | In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on <i>Caenorhabditis elegans</i> |
| title_sort | in vivo and in vitro antioxidant activities of methanol extracts from olive leaves on i caenorhabditis elegans i |
| topic | olive leaves polyphenols antioxidant activities <i>Caenorhabditis elegans</i> thermal stress |
| url | https://www.mdpi.com/1420-3049/24/4/704 |
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