Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology
Abstract Background Cellular physiological responses, which are often obscured by inferences from population-level data, are of great importance in cell biology. Microfluidics has emerged as an important tool for biological research on a small scale, reaching even the single-cell level. Results In t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-10-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-018-0229-1 |
| _version_ | 1819265789920804864 |
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| author | Xudong Zhu Xiang Shi Ju Chu Bangce Ye Peng Zuo Yonghong Wang |
| author_facet | Xudong Zhu Xiang Shi Ju Chu Bangce Ye Peng Zuo Yonghong Wang |
| author_sort | Xudong Zhu |
| collection | DOAJ |
| description | Abstract Background Cellular physiological responses, which are often obscured by inferences from population-level data, are of great importance in cell biology. Microfluidics has emerged as an important tool for biological research on a small scale, reaching even the single-cell level. Results In this work, a flow-focusing microdroplet generator was developed to produce monodisperse microdroplets with high stability. Individual B. coagulans cells were encapsulated in the microdroplets and cultured offline. The specific growth rate of B. coagulans at the single-cell level was analyzed, and the growth of B. coagulans in the droplets showed good consistency with that in flasks, with a correlation coefficient of 0.98. The morphological heterogeneity and its potential relationship with the production of lactic acid by B. coagulans were evaluated using a microscopic imaging method. Conclusion We have demonstrated a single-cell monitoring methodology based on microdroplets. This approach has great potential for studying a range of behavioral and physiological features of bacteria at the single-cell level. |
| first_indexed | 2024-12-23T20:50:58Z |
| format | Article |
| id | doaj.art-96dbb3016b1c4d4db501e7faa34c325e |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-23T20:50:58Z |
| publishDate | 2018-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-96dbb3016b1c4d4db501e7faa34c325e2022-12-21T17:31:40ZengSpringerOpenBioresources and Bioprocessing2197-43652018-10-01511810.1186/s40643-018-0229-1Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technologyXudong Zhu0Xiang Shi1Ju Chu2Bangce Ye3Peng Zuo4Yonghong Wang5State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and TechnologyAbstract Background Cellular physiological responses, which are often obscured by inferences from population-level data, are of great importance in cell biology. Microfluidics has emerged as an important tool for biological research on a small scale, reaching even the single-cell level. Results In this work, a flow-focusing microdroplet generator was developed to produce monodisperse microdroplets with high stability. Individual B. coagulans cells were encapsulated in the microdroplets and cultured offline. The specific growth rate of B. coagulans at the single-cell level was analyzed, and the growth of B. coagulans in the droplets showed good consistency with that in flasks, with a correlation coefficient of 0.98. The morphological heterogeneity and its potential relationship with the production of lactic acid by B. coagulans were evaluated using a microscopic imaging method. Conclusion We have demonstrated a single-cell monitoring methodology based on microdroplets. This approach has great potential for studying a range of behavioral and physiological features of bacteria at the single-cell level.http://link.springer.com/article/10.1186/s40643-018-0229-1Bacillus coagulansMicrodropletsMorphological heterogeneitySingle cell |
| spellingShingle | Xudong Zhu Xiang Shi Ju Chu Bangce Ye Peng Zuo Yonghong Wang Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology Bioresources and Bioprocessing Bacillus coagulans Microdroplets Morphological heterogeneity Single cell |
| title | Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology |
| title_full | Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology |
| title_fullStr | Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology |
| title_full_unstemmed | Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology |
| title_short | Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology |
| title_sort | quantitative analysis of the growth of individual bacillus coagulans cells by microdroplet technology |
| topic | Bacillus coagulans Microdroplets Morphological heterogeneity Single cell |
| url | http://link.springer.com/article/10.1186/s40643-018-0229-1 |
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