A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite
Abstract In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-04-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-017-2026-8 |
| _version_ | 1797727214964310016 |
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| author | O. V. Soldatkina O. O. Soldatkin B. Ozansoy Kasap D. Yu. Kucherenko I. S. Kucherenko B. Akata Kurc S. V. Dzyadevych |
| author_facet | O. V. Soldatkina O. O. Soldatkin B. Ozansoy Kasap D. Yu. Kucherenko I. S. Kucherenko B. Akata Kurc S. V. Dzyadevych |
| author_sort | O. V. Soldatkina |
| collection | DOAJ |
| description | Abstract In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples. |
| first_indexed | 2024-03-12T10:56:38Z |
| format | Article |
| id | doaj.art-96dcde1bcf7f48e8b4e62e5b8af7e9a7 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T10:56:38Z |
| publishDate | 2017-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-96dcde1bcf7f48e8b4e62e5b8af7e9a72023-09-02T06:16:05ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2017-04-011211810.1186/s11671-017-2026-8A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on SilicaliteO. V. Soldatkina0O. O. Soldatkin1B. Ozansoy Kasap2D. Yu. Kucherenko3I. S. Kucherenko4B. Akata Kurc5S. V. Dzyadevych6Institute of High Technologies, Taras Shevchenko National University of KyivInstitute of High Technologies, Taras Shevchenko National University of KyivMicro and Nanotechnology Department, Middle East Technical UniversityInstitute of High Technologies, Taras Shevchenko National University of KyivInstitute of Molecular Biology and Genetics of NAS of UkraineMicro and Nanotechnology Department, Middle East Technical UniversityInstitute of High Technologies, Taras Shevchenko National University of KyivAbstract In this work, we developed a new amperometric biosensor for glutamate detection using a typical method of glutamate oxidase (GlOx) immobilization via adsorption on silicalite particles. The disc platinum electrode (d = 0.4 mm) was used as the amperometric sensor. The procedure of biosensor preparation was optimized. The main parameters of modifying amperometric transducers with a silicalite layer were determined along with the procedure of GlOx adsorption on this layer. The biosensors based on GlOx adsorbed on silicalite demonstrated high sensitivity to glutamate. The linear range of detection was from 2.5 to 450 μM, and the limit of glutamate detection was 1 μM. It was shown that the proposed biosensors were characterized by good response reproducibility during hours of continuous work and operational stability for several days. The developed biosensors could be applied for determination of glutamate in real samples.http://link.springer.com/article/10.1186/s11671-017-2026-8SilicaliteEnzymeBiosensorGlutamate oxidaseAmperometry |
| spellingShingle | O. V. Soldatkina O. O. Soldatkin B. Ozansoy Kasap D. Yu. Kucherenko I. S. Kucherenko B. Akata Kurc S. V. Dzyadevych A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite Nanoscale Research Letters Silicalite Enzyme Biosensor Glutamate oxidase Amperometry |
| title | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
| title_full | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
| title_fullStr | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
| title_full_unstemmed | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
| title_short | A Novel Amperometric Glutamate Biosensor Based on Glutamate Oxidase Adsorbed on Silicalite |
| title_sort | novel amperometric glutamate biosensor based on glutamate oxidase adsorbed on silicalite |
| topic | Silicalite Enzyme Biosensor Glutamate oxidase Amperometry |
| url | http://link.springer.com/article/10.1186/s11671-017-2026-8 |
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