Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> Poiret
<i>Phlomis viscosa</i> Poiret (an evergreen shrub) represents a valuable source of medicinal compounds. In this study, we discovered compounds with antimicrobial and antiviral properties. The aim of this study was to identify compounds of <i>P. viscosa</i> and estimate the an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/2/441 |
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| author | Ludmila Yarmolinsky Faina Nakonechny Arie Budovsky Haim Zeigerman Boris Khalfin Eyal Sharon Leonid Yarmolinsky Shimon Ben-Shabat Marina Nisnevitch |
| author_facet | Ludmila Yarmolinsky Faina Nakonechny Arie Budovsky Haim Zeigerman Boris Khalfin Eyal Sharon Leonid Yarmolinsky Shimon Ben-Shabat Marina Nisnevitch |
| author_sort | Ludmila Yarmolinsky |
| collection | DOAJ |
| description | <i>Phlomis viscosa</i> Poiret (an evergreen shrub) represents a valuable source of medicinal compounds. In this study, we discovered compounds with antimicrobial and antiviral properties. The aim of this study was to identify compounds of <i>P. viscosa</i> and estimate the antimicrobial and antiviral activity of its phytochemicals. The volatile compounds were identified using gas chromatography/mass spectrometry (GC/MS) analysis. For the identification of nonvolatile components of the extracts, high-performance liquid chromatography (HPLC), liquid chromatography–electrospray ionization-mass spectrometry (LC-ESI-MS) and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) were applied. Quercetin 3-<i>O</i>-rutinoside and hesperidin caused a significant decrease in the bacterial concentration of <i>Agrobacterium tumefaciens</i>, <i>Xylella fastidiosa</i> and <i>Pseudomonas syringae</i> (<i>p</i> < 0.001). The growth of drug-resistant microorganisms (<i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Serratia marcescens</i> and <i>Salmonella enteritidis</i>) was inhibited by quercetin 3-<i>O</i>-rutinoside, quercetin 3-<i>O</i>-arabinoside and hesperidin. In addition, these compounds demonstrated antiquorum-sensing properties. Diosmin, hesperidin and quercetin 3-<i>O</i>-arabinoside significantly inhibited varicella zoster virus (VZV) (<i>p</i> < 0.001). Quercetin 3-<i>O</i>-rutinoside and quercetin 3-<i>O</i>-arabinoside were effective against herpes simplex virus 1 (HSV-1), including mutant strains. |
| first_indexed | 2024-03-11T09:07:08Z |
| format | Article |
| id | doaj.art-96dd779588a54828894843dacd7d3f8d |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T09:07:08Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-96dd779588a54828894843dacd7d3f8d2023-11-16T19:18:16ZengMDPI AGBiomedicines2227-90592023-02-0111244110.3390/biomedicines11020441Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> PoiretLudmila Yarmolinsky0Faina Nakonechny1Arie Budovsky2Haim Zeigerman3Boris Khalfin4Eyal Sharon5Leonid Yarmolinsky6Shimon Ben-Shabat7Marina Nisnevitch8Eastern R&D Center, Kiryat Arba 9010000, IsraelDepartment of Chemical Engineering, Ariel University, Ariel 4070000, IsraelResearch & Development Authority, Barzilai University Medical Center, Ashkelon 7830604, IsraelInstitute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot 7610001, IsraelEastern R&D Center, Kiryat Arba 9010000, IsraelInstitute for Personalized and Translational Medicine, Ariel University, Ariel 4070000, IsraelArnie Miller Laboratories, Beer-Sheva 8430713, IsraelFaculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8410501, IsraelDepartment of Chemical Engineering, Ariel University, Ariel 4070000, Israel<i>Phlomis viscosa</i> Poiret (an evergreen shrub) represents a valuable source of medicinal compounds. In this study, we discovered compounds with antimicrobial and antiviral properties. The aim of this study was to identify compounds of <i>P. viscosa</i> and estimate the antimicrobial and antiviral activity of its phytochemicals. The volatile compounds were identified using gas chromatography/mass spectrometry (GC/MS) analysis. For the identification of nonvolatile components of the extracts, high-performance liquid chromatography (HPLC), liquid chromatography–electrospray ionization-mass spectrometry (LC-ESI-MS) and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) were applied. Quercetin 3-<i>O</i>-rutinoside and hesperidin caused a significant decrease in the bacterial concentration of <i>Agrobacterium tumefaciens</i>, <i>Xylella fastidiosa</i> and <i>Pseudomonas syringae</i> (<i>p</i> < 0.001). The growth of drug-resistant microorganisms (<i>Escherichia coli</i>, <i>Klebsiella pneumoniae</i>, <i>Acinetobacter baumannii</i>, <i>Serratia marcescens</i> and <i>Salmonella enteritidis</i>) was inhibited by quercetin 3-<i>O</i>-rutinoside, quercetin 3-<i>O</i>-arabinoside and hesperidin. In addition, these compounds demonstrated antiquorum-sensing properties. Diosmin, hesperidin and quercetin 3-<i>O</i>-arabinoside significantly inhibited varicella zoster virus (VZV) (<i>p</i> < 0.001). Quercetin 3-<i>O</i>-rutinoside and quercetin 3-<i>O</i>-arabinoside were effective against herpes simplex virus 1 (HSV-1), including mutant strains.https://www.mdpi.com/2227-9059/11/2/441<i>Phlomis viscosa</i>antimicrobial compoundsantiviral compoundsflavonoidsdrug-resistant microorganismsbiofilm formation |
| spellingShingle | Ludmila Yarmolinsky Faina Nakonechny Arie Budovsky Haim Zeigerman Boris Khalfin Eyal Sharon Leonid Yarmolinsky Shimon Ben-Shabat Marina Nisnevitch Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> Poiret Biomedicines <i>Phlomis viscosa</i> antimicrobial compounds antiviral compounds flavonoids drug-resistant microorganisms biofilm formation |
| title | Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> Poiret |
| title_full | Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> Poiret |
| title_fullStr | Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> Poiret |
| title_full_unstemmed | Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> Poiret |
| title_short | Antimicrobial and Antiviral Compounds of <i>Phlomis viscosa</i> Poiret |
| title_sort | antimicrobial and antiviral compounds of i phlomis viscosa i poiret |
| topic | <i>Phlomis viscosa</i> antimicrobial compounds antiviral compounds flavonoids drug-resistant microorganisms biofilm formation |
| url | https://www.mdpi.com/2227-9059/11/2/441 |
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