Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration.
Hair follicle stem cells (HFSCs) are multipotent cells that cycle through quiescence and activation to continuously fuel the production of hair follicles. Prior genome mapping studies had shown that tri-methylation of histone H3 at lysine 27 (H3K27me3), the chromatin mark mediated by Polycomb Repres...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-12-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009948 |
| _version_ | 1811292742652788736 |
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| author | Pooja Flora Meng-Yen Li Phillip M Galbo Maider Astorkia Deyou Zheng Elena Ezhkova |
| author_facet | Pooja Flora Meng-Yen Li Phillip M Galbo Maider Astorkia Deyou Zheng Elena Ezhkova |
| author_sort | Pooja Flora |
| collection | DOAJ |
| description | Hair follicle stem cells (HFSCs) are multipotent cells that cycle through quiescence and activation to continuously fuel the production of hair follicles. Prior genome mapping studies had shown that tri-methylation of histone H3 at lysine 27 (H3K27me3), the chromatin mark mediated by Polycomb Repressive Complex 2 (PRC2), is dynamic between quiescent and activated HFSCs, suggesting that transcriptional changes associated with H3K27me3 might be critical for proper HFSC function. However, functional in vivo studies elucidating the role of PRC2 in adult HFSCs are lacking. In this study, by using in vivo loss-of-function studies we show that, surprisingly, PRC2 plays a non-instructive role in adult HFSCs and loss of PRC2 in HFSCs does not lead to loss of HFSC quiescence or changes in cell identity. Interestingly, RNA-seq and immunofluorescence analyses of PRC2-null quiescent HFSCs revealed upregulation of genes associated with activated state of HFSCs. Altogether, our findings show that transcriptional program under PRC2 regulation is dispensable for maintaining HFSC quiescence and hair regeneration. |
| first_indexed | 2024-04-13T04:50:51Z |
| format | Article |
| id | doaj.art-96e3077b6b50440984281239a8c93471 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T04:50:51Z |
| publishDate | 2021-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-96e3077b6b50440984281239a8c934712022-12-22T03:01:41ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-12-011712e100994810.1371/journal.pgen.1009948Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration.Pooja FloraMeng-Yen LiPhillip M GalboMaider AstorkiaDeyou ZhengElena EzhkovaHair follicle stem cells (HFSCs) are multipotent cells that cycle through quiescence and activation to continuously fuel the production of hair follicles. Prior genome mapping studies had shown that tri-methylation of histone H3 at lysine 27 (H3K27me3), the chromatin mark mediated by Polycomb Repressive Complex 2 (PRC2), is dynamic between quiescent and activated HFSCs, suggesting that transcriptional changes associated with H3K27me3 might be critical for proper HFSC function. However, functional in vivo studies elucidating the role of PRC2 in adult HFSCs are lacking. In this study, by using in vivo loss-of-function studies we show that, surprisingly, PRC2 plays a non-instructive role in adult HFSCs and loss of PRC2 in HFSCs does not lead to loss of HFSC quiescence or changes in cell identity. Interestingly, RNA-seq and immunofluorescence analyses of PRC2-null quiescent HFSCs revealed upregulation of genes associated with activated state of HFSCs. Altogether, our findings show that transcriptional program under PRC2 regulation is dispensable for maintaining HFSC quiescence and hair regeneration.https://doi.org/10.1371/journal.pgen.1009948 |
| spellingShingle | Pooja Flora Meng-Yen Li Phillip M Galbo Maider Astorkia Deyou Zheng Elena Ezhkova Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration. PLoS Genetics |
| title | Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration. |
| title_full | Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration. |
| title_fullStr | Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration. |
| title_full_unstemmed | Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration. |
| title_short | Polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration. |
| title_sort | polycomb repressive complex 2 in adult hair follicle stem cells is dispensable for hair regeneration |
| url | https://doi.org/10.1371/journal.pgen.1009948 |
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