Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies
Seaweeds are a rich source of protein and can contain up to 47% on the dry weight basis. It is challenging to extract proteins from the raw biomass of seaweed due to resilient cell-wall complexes. Four species of macroalgae were used in this study-two brown, <i>Fucus vesiculosus</i> and...
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MDPI AG
2020-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/8/2005 |
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| author | Jack O’ Connor Steve Meaney Gwilym A. Williams Maria Hayes |
| author_facet | Jack O’ Connor Steve Meaney Gwilym A. Williams Maria Hayes |
| author_sort | Jack O’ Connor |
| collection | DOAJ |
| description | Seaweeds are a rich source of protein and can contain up to 47% on the dry weight basis. It is challenging to extract proteins from the raw biomass of seaweed due to resilient cell-wall complexes. Four species of macroalgae were used in this study-two brown, <i>Fucus vesiculosus</i> and <i>Alaria esculenta</i>, and two red, <i>Palmaria palmata</i> and <i>Chondrus crispus</i>. Three treatments were applied individually to the macroalgal species: (I) high-pressure processing (HPP); (II) laboratory autoclave processing and (III) a classical sonication and salting out method. The protein, ash and lipid contents of the resulting extracts were estimated. Yields of protein recovered ranged from 3.2% for <i>Fucus vesiculosus</i> pre-treated with high pressure processing to 28.9% protein recovered for <i>Chondrus crispus</i> treated with the classical method. The yields of protein recovered using the classical, HPP and autoclave pre-treatments applied to <i>Fucus vesiculosus</i> were 35.1, 23.7% and 24.3%, respectively; yields from <i>Alaria esculenta</i> were 18.2%, 15.0% and 17.1% respectively; yields from <i>Palmaria palmata</i> were 12.5%, 14.9% and 21.5% respectively, and finally, yields from <i>Chondrus crispus</i> were 35.2%, 16.1% and 21.9%, respectively. These results demonstrate that while macroalgal proteins may be extracted using either physical or enzymatic methods, the specific extraction procedure should be tailored to individual species. |
| first_indexed | 2024-03-10T20:15:38Z |
| format | Article |
| id | doaj.art-96e431ba5b6d4a2cba94a56695d41ce1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T20:15:38Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-96e431ba5b6d4a2cba94a56695d41ce12023-11-19T22:38:28ZengMDPI AGMolecules1420-30492020-04-01258200510.3390/molecules25082005Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment StrategiesJack O’ Connor0Steve Meaney1Gwilym A. Williams2Maria Hayes3The Food BioSciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, IrelandSchool of Biological and Health Sciences, Technological University Dublin (TU Dublin)–City Campus, Kevin Street, Dublin 2, IrelandSchool of Biological and Health Sciences, Technological University Dublin (TU Dublin)–City Campus, Kevin Street, Dublin 2, IrelandThe Food BioSciences Department, Teagasc Food Research Centre, Ashtown, Dublin 15, IrelandSeaweeds are a rich source of protein and can contain up to 47% on the dry weight basis. It is challenging to extract proteins from the raw biomass of seaweed due to resilient cell-wall complexes. Four species of macroalgae were used in this study-two brown, <i>Fucus vesiculosus</i> and <i>Alaria esculenta</i>, and two red, <i>Palmaria palmata</i> and <i>Chondrus crispus</i>. Three treatments were applied individually to the macroalgal species: (I) high-pressure processing (HPP); (II) laboratory autoclave processing and (III) a classical sonication and salting out method. The protein, ash and lipid contents of the resulting extracts were estimated. Yields of protein recovered ranged from 3.2% for <i>Fucus vesiculosus</i> pre-treated with high pressure processing to 28.9% protein recovered for <i>Chondrus crispus</i> treated with the classical method. The yields of protein recovered using the classical, HPP and autoclave pre-treatments applied to <i>Fucus vesiculosus</i> were 35.1, 23.7% and 24.3%, respectively; yields from <i>Alaria esculenta</i> were 18.2%, 15.0% and 17.1% respectively; yields from <i>Palmaria palmata</i> were 12.5%, 14.9% and 21.5% respectively, and finally, yields from <i>Chondrus crispus</i> were 35.2%, 16.1% and 21.9%, respectively. These results demonstrate that while macroalgal proteins may be extracted using either physical or enzymatic methods, the specific extraction procedure should be tailored to individual species.https://www.mdpi.com/1420-3049/25/8/2005seaweedsproteinsautoclavehigh pressure processingtraditional protein extractiontotal and free amino acids |
| spellingShingle | Jack O’ Connor Steve Meaney Gwilym A. Williams Maria Hayes Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies Molecules seaweeds proteins autoclave high pressure processing traditional protein extraction total and free amino acids |
| title | Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies |
| title_full | Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies |
| title_fullStr | Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies |
| title_full_unstemmed | Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies |
| title_short | Extraction of Protein from Four Different Seaweeds Using Three Different Physical Pre-Treatment Strategies |
| title_sort | extraction of protein from four different seaweeds using three different physical pre treatment strategies |
| topic | seaweeds proteins autoclave high pressure processing traditional protein extraction total and free amino acids |
| url | https://www.mdpi.com/1420-3049/25/8/2005 |
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