Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array Detector
Cheongsangbangpung-tang (CSBPT) is a traditional herbal medicine that has been used in many regions of Asia, including Korea, China, and Japan, for the treatment of purulent inflammation and eczema on the face. In this study, a method for the simultaneous analysis of 18 marker components, geniposide...
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MDPI AG
2020-12-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/11/1/14 |
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| author | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_facet | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_sort | Chang-Seob Seo |
| collection | DOAJ |
| description | Cheongsangbangpung-tang (CSBPT) is a traditional herbal medicine that has been used in many regions of Asia, including Korea, China, and Japan, for the treatment of purulent inflammation and eczema on the face. In this study, a method for the simultaneous analysis of 18 marker components, geniposide (<b>1</b>), coptisine chloride (<b>2</b>), <i>prim</i>-<i>O</i>-glucosylcimifugin (<b>3</b>), berberine chloride (<b>4</b>), liquiritin apioside (<b>5</b>), liquiritin (<b>6</b>), ferulic acid (<b>7</b>), narirutin (<b>8</b>), 5-<i>O</i>-methylvisammisoide (<b>9</b>), hesperidin (<b>10</b>), arctigenin (<b>11</b>), baicalin (<b>12</b>), oxypeucedanin hydrate (<b>13</b>), wogonoside (<b>14</b>), baicalein (<b>15</b>), arctiin (<b>16</b>), glycyrrhizin (<b>17</b>), and pulegone (<b>18</b>), was developed for quality control of CSBPT. The novel approach, which is based on high-performance liquid chromatography (HPLC) separation coupled with photodiode array detection, was verified by the assessment of linearity, limit of detection, limit of quantification, accuracy, recovery, and precision. Analysis of CSBPT by using the established assay revealed that compounds <b>1</b>–<b>18</b> were present in concentrations of 0.27–18.31 mg/g. |
| first_indexed | 2024-03-10T13:52:26Z |
| format | Article |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T13:52:26Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-96e5aee465164a6980f9e8511a8283002023-11-21T02:03:22ZengMDPI AGApplied Sciences2076-34172020-12-011111410.3390/app11010014Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array DetectorChang-Seob Seo0Hyeun-Kyoo Shin1Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaHerbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, KoreaCheongsangbangpung-tang (CSBPT) is a traditional herbal medicine that has been used in many regions of Asia, including Korea, China, and Japan, for the treatment of purulent inflammation and eczema on the face. In this study, a method for the simultaneous analysis of 18 marker components, geniposide (<b>1</b>), coptisine chloride (<b>2</b>), <i>prim</i>-<i>O</i>-glucosylcimifugin (<b>3</b>), berberine chloride (<b>4</b>), liquiritin apioside (<b>5</b>), liquiritin (<b>6</b>), ferulic acid (<b>7</b>), narirutin (<b>8</b>), 5-<i>O</i>-methylvisammisoide (<b>9</b>), hesperidin (<b>10</b>), arctigenin (<b>11</b>), baicalin (<b>12</b>), oxypeucedanin hydrate (<b>13</b>), wogonoside (<b>14</b>), baicalein (<b>15</b>), arctiin (<b>16</b>), glycyrrhizin (<b>17</b>), and pulegone (<b>18</b>), was developed for quality control of CSBPT. The novel approach, which is based on high-performance liquid chromatography (HPLC) separation coupled with photodiode array detection, was verified by the assessment of linearity, limit of detection, limit of quantification, accuracy, recovery, and precision. Analysis of CSBPT by using the established assay revealed that compounds <b>1</b>–<b>18</b> were present in concentrations of 0.27–18.31 mg/g.https://www.mdpi.com/2076-3417/11/1/14quantitative analysisCheongsangbangpung-tangpurulent inflammationhigh-performance liquid chromatography |
| spellingShingle | Chang-Seob Seo Hyeun-Kyoo Shin Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array Detector Applied Sciences quantitative analysis Cheongsangbangpung-tang purulent inflammation high-performance liquid chromatography |
| title | Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array Detector |
| title_full | Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array Detector |
| title_fullStr | Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array Detector |
| title_full_unstemmed | Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array Detector |
| title_short | Quantitative Analysis of 18 Marker Components in the Traditional Korean Medicine, Cheongsangbangpung-Tang, Using High-Performance Liquid Chromatography Combined with Photodiode Array Detector |
| title_sort | quantitative analysis of 18 marker components in the traditional korean medicine cheongsangbangpung tang using high performance liquid chromatography combined with photodiode array detector |
| topic | quantitative analysis Cheongsangbangpung-tang purulent inflammation high-performance liquid chromatography |
| url | https://www.mdpi.com/2076-3417/11/1/14 |
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