Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia
After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/15/8093 |
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| author | Júlia Faura Laura Ramiro Alba Simats Feifei Ma Anna Penalba Teresa Gasull Anna Rosell Joan Montaner Alejandro Bustamante |
| author_facet | Júlia Faura Laura Ramiro Alba Simats Feifei Ma Anna Penalba Teresa Gasull Anna Rosell Joan Montaner Alejandro Bustamante |
| author_sort | Júlia Faura |
| collection | DOAJ |
| description | After stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics<sup>®</sup> was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than <i>sham</i>-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage. |
| first_indexed | 2024-03-09T10:08:44Z |
| format | Article |
| id | doaj.art-96e669d8ad264645ac8658e0ae01a5ea |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:08:44Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-96e669d8ad264645ac8658e0ae01a5ea2023-12-01T22:56:42ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012315809310.3390/ijms23158093Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral IschemiaJúlia Faura0Laura Ramiro1Alba Simats2Feifei Ma3Anna Penalba4Teresa Gasull5Anna Rosell6Joan Montaner7Alejandro Bustamante8Neurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainNeurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainNeurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainNeurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainNeurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainCellular and Molecular Neurobiology Research Group, Department of Neurosciences, Germans Trias i Pujol Research Institute (IGTP), 08916 Badalona, SpainNeurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainNeurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainNeurovascular Research Laboratory, Valld’Hebron Research Institute (VHIR), Universitat Autònoma de Barcelona (UAB), 08035 Barcelona, SpainAfter stroke and other brain injuries, there is a high incidence of respiratory complications such as pneumonia or acute lung injury. The molecular mechanisms that drive the brain-lung interaction post-stroke have not yet been elucidated. We performed transient middle cerebral artery occlusion (MCAO) and sham surgery on C57BL/6J mice and collected bronchoalveolar lavage fluid (BALF), serum, brain, and lung homogenate samples 24 h after surgery. A 92 proteins-panel developed by Olink Proteomics<sup>®</sup> was used to analyze the content in BALF and lung homogenates. MCAO animals had higher protein concentration levels in BALF than <i>sham</i>-controls, but these levels did not correlate with the infarct volume. No alteration in alveolar-capillary barrier permeability was observed. A total of 12 and 14 proteins were differentially expressed between the groups (FDR < 0.1) in BALF and lung tissue homogenates, respectively. Of those, HGF, TGF-α, and CCL2 were identified as the most relevant to this study. Their protein expression patterns were verified by ELISA. This study confirmed that post-stroke lung damage was not associated with increased lung permeability or cerebral ischemia severity. Furthermore, the dysregulation of HGF, TGF-α, and CCL2 in BALF and lung tissue after ischemia could play an important role in the molecular mechanisms underlying stroke-induced lung damage.https://www.mdpi.com/1422-0067/23/15/8093strokeinflammationpulmonary complications |
| spellingShingle | Júlia Faura Laura Ramiro Alba Simats Feifei Ma Anna Penalba Teresa Gasull Anna Rosell Joan Montaner Alejandro Bustamante Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia International Journal of Molecular Sciences stroke inflammation pulmonary complications |
| title | Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia |
| title_full | Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia |
| title_fullStr | Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia |
| title_full_unstemmed | Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia |
| title_short | Evaluation and Characterization of Post-Stroke Lung Damage in a Murine Model of Cerebral Ischemia |
| title_sort | evaluation and characterization of post stroke lung damage in a murine model of cerebral ischemia |
| topic | stroke inflammation pulmonary complications |
| url | https://www.mdpi.com/1422-0067/23/15/8093 |
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