Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing
Abstract Background Long‐term overgrazing has led to severe degradation of grasslands, posing a significant threat to the sustainable use of grassland resources. Methods Based on the investigation of changes in functional traits and photosynthetic physiology of Stipa breviflora under no grazing, mod...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Grassland Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/glr2.12071 |
| _version_ | 1797198236305326080 |
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| author | Yanan Liu Shixian Sun Yanan Zhang Miaomiao Song Yunyun Tian Peter J. Lockhart Xin Zhang Ying Xu Zhenhua Dang |
| author_facet | Yanan Liu Shixian Sun Yanan Zhang Miaomiao Song Yunyun Tian Peter J. Lockhart Xin Zhang Ying Xu Zhenhua Dang |
| author_sort | Yanan Liu |
| collection | DOAJ |
| description | Abstract Background Long‐term overgrazing has led to severe degradation of grasslands, posing a significant threat to the sustainable use of grassland resources. Methods Based on the investigation of changes in functional traits and photosynthetic physiology of Stipa breviflora under no grazing, moderate grazing, and heavy grazing treatments, the changes in expression patterns of genes and proteins associated with different grazing intensities were assessed through integrative transcriptomic and proteomic analyses. Results Differentially expressed genes and proteins were identified under different grazing intensities. They were mainly related to RNA processing, carbon metabolism, and secondary metabolite biosynthesis. These findings suggest that long‐term grazing leads to molecular phenotypic plasticity, affecting various biological processes and metabolic pathways in S. breviflora. Correlation analysis revealed low correlation between the transcriptome and the proteome, indicating a large‐scale regulation of gene expression at the posttranscriptional and translational levels during the response of S. breviflora to grazing. The expression profiles of key genes and proteins involved in photosynthesis and phenylpropanoid metabolism pathways suggested their synergistic response to grazing in S. breviflora. Conclusions Our study provides insight into the adaptation mechanisms of S. breviflora to grazing and provides a scientific basis for the development of more efficient grassland protection and utilization practices. |
| first_indexed | 2024-04-24T06:56:39Z |
| format | Article |
| id | doaj.art-96e86652112f4bbfafab0def52b28e62 |
| institution | Directory Open Access Journal |
| issn | 2097-051X 2770-1743 |
| language | English |
| last_indexed | 2024-04-24T06:56:39Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Grassland Research |
| spelling | doaj.art-96e86652112f4bbfafab0def52b28e622024-04-22T11:04:50ZengWileyGrassland Research2097-051X2770-17432024-03-013111710.1002/glr2.12071Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazingYanan Liu0Shixian Sun1Yanan Zhang2Miaomiao Song3Yunyun Tian4Peter J. Lockhart5Xin Zhang6Ying Xu7Zhenhua Dang8Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment Inner Mongolia University Hohhot Inner Mongolia ChinaInstitute of Grassland Research Chinese Academy of Agricultural Sciences Hohhot Inner Mongolia ChinaMinistry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment Inner Mongolia University Hohhot Inner Mongolia ChinaMinistry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment Inner Mongolia University Hohhot Inner Mongolia ChinaMinistry of Education Key Laboratory of Herbage & Endemic Crop Biotechnology, School of Life Sciences Inner Mongolia University Hohhot Inner Mongolia ChinaSchool of Natural Sciences, College of Sciences Massey University Palmerston North New ZealandMinistry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment Inner Mongolia University Hohhot Inner Mongolia ChinaMinistry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment Inner Mongolia University Hohhot Inner Mongolia ChinaMinistry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau & Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment Inner Mongolia University Hohhot Inner Mongolia ChinaAbstract Background Long‐term overgrazing has led to severe degradation of grasslands, posing a significant threat to the sustainable use of grassland resources. Methods Based on the investigation of changes in functional traits and photosynthetic physiology of Stipa breviflora under no grazing, moderate grazing, and heavy grazing treatments, the changes in expression patterns of genes and proteins associated with different grazing intensities were assessed through integrative transcriptomic and proteomic analyses. Results Differentially expressed genes and proteins were identified under different grazing intensities. They were mainly related to RNA processing, carbon metabolism, and secondary metabolite biosynthesis. These findings suggest that long‐term grazing leads to molecular phenotypic plasticity, affecting various biological processes and metabolic pathways in S. breviflora. Correlation analysis revealed low correlation between the transcriptome and the proteome, indicating a large‐scale regulation of gene expression at the posttranscriptional and translational levels during the response of S. breviflora to grazing. The expression profiles of key genes and proteins involved in photosynthesis and phenylpropanoid metabolism pathways suggested their synergistic response to grazing in S. breviflora. Conclusions Our study provides insight into the adaptation mechanisms of S. breviflora to grazing and provides a scientific basis for the development of more efficient grassland protection and utilization practices.https://doi.org/10.1002/glr2.12071functional traitsgrazing adaptationphotosynthetic physiologyproteomeStipa brevifloratranscriptome |
| spellingShingle | Yanan Liu Shixian Sun Yanan Zhang Miaomiao Song Yunyun Tian Peter J. Lockhart Xin Zhang Ying Xu Zhenhua Dang Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing Grassland Research functional traits grazing adaptation photosynthetic physiology proteome Stipa breviflora transcriptome |
| title | Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing |
| title_full | Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing |
| title_fullStr | Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing |
| title_full_unstemmed | Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing |
| title_short | Integrated transcriptome and proteome analyses reveal potential mechanisms in Stipa breviflora underlying adaptation to grazing |
| title_sort | integrated transcriptome and proteome analyses reveal potential mechanisms in stipa breviflora underlying adaptation to grazing |
| topic | functional traits grazing adaptation photosynthetic physiology proteome Stipa breviflora transcriptome |
| url | https://doi.org/10.1002/glr2.12071 |
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