Accurate point-of-care serology tests for COVID-19.
<h4>Background</h4>As COVID-19 vaccines become available, screening individuals for prior COVID-19 infection and vaccine response in point-of-care (POC) settings has renewed interest. We prospectively screened at-risk individuals for SARS-CoV-2 spike and nucleocapsid protein antibodies i...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0248729&type=printable |
| _version_ | 1826554308930306048 |
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| author | Charles F Schuler Carmen Gherasim Kelly O'Shea David M Manthei Jesse Chen Don Giacherio Jonathan P Troost James L Baldwin James R Baker |
| author_facet | Charles F Schuler Carmen Gherasim Kelly O'Shea David M Manthei Jesse Chen Don Giacherio Jonathan P Troost James L Baldwin James R Baker |
| author_sort | Charles F Schuler |
| collection | DOAJ |
| description | <h4>Background</h4>As COVID-19 vaccines become available, screening individuals for prior COVID-19 infection and vaccine response in point-of-care (POC) settings has renewed interest. We prospectively screened at-risk individuals for SARS-CoV-2 spike and nucleocapsid protein antibodies in a POC setting to determine if it was a feasible method to identify antibody from prior infection.<h4>Methods</h4>Three EUA-approved lateral flow antibody assays were performed on POC finger-stick blood and compared with serum and a CLIA nucleocapsid antibody immunoassay. Variables including antibody class, time since PCR, and the assay antigen used were evaluated.<h4>Results</h4>512 subjects enrolled, of which 104 had a COVID-19 history and positive PCR. Only three PCR-positive subjects required hospitalization, with one requiring mechanical ventilation. The POC results correlated well with the immunoassay (93-97% sensitivity) and using serum did not improve the sensitivity or specificity.<h4>Conclusions</h4>Finger-stick, POC COVID-19 antibody testing was highly effective in identifying antibody resulting from prior infections in mildly symptomatic subjects. Using high-complexity serum immunoassays did not improve the screening outcome. Almost all individuals with COVID-19 infection produced detectable antibodies to the virus. POC antibody testing is useful as a screen for prior COVID-19 infection, and should be useful in assessing vaccine response. |
| first_indexed | 2024-03-13T10:18:59Z |
| format | Article |
| id | doaj.art-96e8a5c980564ecf91b92c95ba97a12b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T07:38:51Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-96e8a5c980564ecf91b92c95ba97a12b2025-03-03T05:36:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01163e024872910.1371/journal.pone.0248729Accurate point-of-care serology tests for COVID-19.Charles F SchulerCarmen GherasimKelly O'SheaDavid M MantheiJesse ChenDon GiacherioJonathan P TroostJames L BaldwinJames R Baker<h4>Background</h4>As COVID-19 vaccines become available, screening individuals for prior COVID-19 infection and vaccine response in point-of-care (POC) settings has renewed interest. We prospectively screened at-risk individuals for SARS-CoV-2 spike and nucleocapsid protein antibodies in a POC setting to determine if it was a feasible method to identify antibody from prior infection.<h4>Methods</h4>Three EUA-approved lateral flow antibody assays were performed on POC finger-stick blood and compared with serum and a CLIA nucleocapsid antibody immunoassay. Variables including antibody class, time since PCR, and the assay antigen used were evaluated.<h4>Results</h4>512 subjects enrolled, of which 104 had a COVID-19 history and positive PCR. Only three PCR-positive subjects required hospitalization, with one requiring mechanical ventilation. The POC results correlated well with the immunoassay (93-97% sensitivity) and using serum did not improve the sensitivity or specificity.<h4>Conclusions</h4>Finger-stick, POC COVID-19 antibody testing was highly effective in identifying antibody resulting from prior infections in mildly symptomatic subjects. Using high-complexity serum immunoassays did not improve the screening outcome. Almost all individuals with COVID-19 infection produced detectable antibodies to the virus. POC antibody testing is useful as a screen for prior COVID-19 infection, and should be useful in assessing vaccine response.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0248729&type=printable |
| spellingShingle | Charles F Schuler Carmen Gherasim Kelly O'Shea David M Manthei Jesse Chen Don Giacherio Jonathan P Troost James L Baldwin James R Baker Accurate point-of-care serology tests for COVID-19. PLoS ONE |
| title | Accurate point-of-care serology tests for COVID-19. |
| title_full | Accurate point-of-care serology tests for COVID-19. |
| title_fullStr | Accurate point-of-care serology tests for COVID-19. |
| title_full_unstemmed | Accurate point-of-care serology tests for COVID-19. |
| title_short | Accurate point-of-care serology tests for COVID-19. |
| title_sort | accurate point of care serology tests for covid 19 |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0248729&type=printable |
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