Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in Poland
The aim of this study was the isolation and characterization, including the phage effect on honeybees in laboratory conditions, of phages active against <i>Paenibacillus larvae</i>, the causative agent of American Foulbrood—a highly infective and easily spreading disease occurring in hon...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/7/1217 |
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| author | Ewa Jończyk-Matysiak Barbara Owczarek Ewa Popiela Kinga Świtała-Jeleń Paweł Migdał Martyna Cieślik Norbert Łodej Dominika Kula Joanna Neuberg Katarzyna Hodyra-Stefaniak Marta Kaszowska Filip Orwat Natalia Bagińska Anna Mucha Agnieszka Belter Mirosława Skupińska Barbara Bubak Wojciech Fortuna Sławomir Letkiewicz Paweł Chorbiński Beata Weber-Dąbrowska Adam Roman Andrzej Górski |
| author_facet | Ewa Jończyk-Matysiak Barbara Owczarek Ewa Popiela Kinga Świtała-Jeleń Paweł Migdał Martyna Cieślik Norbert Łodej Dominika Kula Joanna Neuberg Katarzyna Hodyra-Stefaniak Marta Kaszowska Filip Orwat Natalia Bagińska Anna Mucha Agnieszka Belter Mirosława Skupińska Barbara Bubak Wojciech Fortuna Sławomir Letkiewicz Paweł Chorbiński Beata Weber-Dąbrowska Adam Roman Andrzej Górski |
| author_sort | Ewa Jończyk-Matysiak |
| collection | DOAJ |
| description | The aim of this study was the isolation and characterization, including the phage effect on honeybees in laboratory conditions, of phages active against <i>Paenibacillus larvae</i>, the causative agent of American Foulbrood—a highly infective and easily spreading disease occurring in honeybee larva, and subsequently the development of a preparation to prevent and treat this dangerous disease. From the tested material (over 2500 samples) 35 <i>Paenibacillus</i> spp. strains were obtained and used to search for phages. Five phages specific to <i>Paenibacillus</i> were isolated and characterized (ultrastructure, morphology, biological properties, storage stability, and genome sequence). The characteristics were performed to obtain knowledge of their lytic potential and compose the final phage cocktail with high antibacterial potential and intended use of future field application. Preliminary safety studies have also been carried out on healthy bees, which suggest that the phage preparation administered is harmless. |
| first_indexed | 2024-03-10T10:06:43Z |
| format | Article |
| id | doaj.art-96e977e44fb04b2fb9bd005c58e6551a |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T10:06:43Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-96e977e44fb04b2fb9bd005c58e6551a2023-11-22T01:27:51ZengMDPI AGViruses1999-49152021-06-01137121710.3390/v13071217Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in PolandEwa Jończyk-Matysiak0Barbara Owczarek1Ewa Popiela2Kinga Świtała-Jeleń3Paweł Migdał4Martyna Cieślik5Norbert Łodej6Dominika Kula7Joanna Neuberg8Katarzyna Hodyra-Stefaniak9Marta Kaszowska10Filip Orwat11Natalia Bagińska12Anna Mucha13Agnieszka Belter14Mirosława Skupińska15Barbara Bubak16Wojciech Fortuna17Sławomir Letkiewicz18Paweł Chorbiński19Beata Weber-Dąbrowska20Adam Roman21Andrzej Górski22Bacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandDepartment of Environment Hygiene and Animal Welfare, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 38C, 51-630 Wroclaw, PolandPure Biologics, Duńska Street 11, 54-427 Wroclaw, PolandDepartment of Environment Hygiene and Animal Welfare, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 38C, 51-630 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandPure Biologics, Duńska Street 11, 54-427 Wroclaw, PolandLaboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 54-427 Wrocław, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandDepartment of Genetics, Wrocław University of Environmental and Life Sciences, Kożuchowska 7, 51-631 Wroclaw, PolandBioScientia, Ogrodowa Street 2/8, 61-820 Poznań, PolandBioScientia, Ogrodowa Street 2/8, 61-820 Poznań, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandDepartment of Neurosurgery, Wrocław Medical University, Borowska 213, 54-427 Wrocław, PolandPhage Therapy Unit, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandDepartment of Epizootiology and Clinic of Birds and Exotic Animals, Wrocław University of Environmental and Life Sciences, pl. Grunwaldzki 45, 50-366 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandDepartment of Environment Hygiene and Animal Welfare, Wrocław University of Environmental and Life Sciences, Chełmońskiego Street 38C, 51-630 Wroclaw, PolandBacteriophage Laboratory, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolf Weigl Street 12, 53-114 Wroclaw, PolandThe aim of this study was the isolation and characterization, including the phage effect on honeybees in laboratory conditions, of phages active against <i>Paenibacillus larvae</i>, the causative agent of American Foulbrood—a highly infective and easily spreading disease occurring in honeybee larva, and subsequently the development of a preparation to prevent and treat this dangerous disease. From the tested material (over 2500 samples) 35 <i>Paenibacillus</i> spp. strains were obtained and used to search for phages. Five phages specific to <i>Paenibacillus</i> were isolated and characterized (ultrastructure, morphology, biological properties, storage stability, and genome sequence). The characteristics were performed to obtain knowledge of their lytic potential and compose the final phage cocktail with high antibacterial potential and intended use of future field application. Preliminary safety studies have also been carried out on healthy bees, which suggest that the phage preparation administered is harmless.https://www.mdpi.com/1999-4915/13/7/1217American foulbroodhoneybeebacteriophagesphage isolation<i>Paenibacillus larvae</i> |
| spellingShingle | Ewa Jończyk-Matysiak Barbara Owczarek Ewa Popiela Kinga Świtała-Jeleń Paweł Migdał Martyna Cieślik Norbert Łodej Dominika Kula Joanna Neuberg Katarzyna Hodyra-Stefaniak Marta Kaszowska Filip Orwat Natalia Bagińska Anna Mucha Agnieszka Belter Mirosława Skupińska Barbara Bubak Wojciech Fortuna Sławomir Letkiewicz Paweł Chorbiński Beata Weber-Dąbrowska Adam Roman Andrzej Górski Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in Poland Viruses American foulbrood honeybee bacteriophages phage isolation <i>Paenibacillus larvae</i> |
| title | Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in Poland |
| title_full | Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in Poland |
| title_fullStr | Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in Poland |
| title_full_unstemmed | Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in Poland |
| title_short | Isolation and Characterization of Phages Active against <i>Paenibacillus larva</i><i>e</i> Causing American Foulbrood in Honeybees in Poland |
| title_sort | isolation and characterization of phages active against i paenibacillus larva i i e i causing american foulbrood in honeybees in poland |
| topic | American foulbrood honeybee bacteriophages phage isolation <i>Paenibacillus larvae</i> |
| url | https://www.mdpi.com/1999-4915/13/7/1217 |
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