Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings
Abstract Background About 2% of pregnant women receive non-obstetric surgery under general anesthesia each year. During pregnancy, general anesthetics may affect brain development of the fetus. This study aimed to investigate safe dosage range of isoflurane. Methods Forty-eight SpragueDawley (SD) pr...
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Anesthesiology |
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| Online Access: | http://link.springer.com/article/10.1186/s12871-018-0471-2 |
| _version_ | 1828516120701698048 |
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| author | Wei Huang Yunxia Dong Guangyi Zhao Yuan Wang Jingjing Jiang Ping Zhao |
| author_facet | Wei Huang Yunxia Dong Guangyi Zhao Yuan Wang Jingjing Jiang Ping Zhao |
| author_sort | Wei Huang |
| collection | DOAJ |
| description | Abstract Background About 2% of pregnant women receive non-obstetric surgery under general anesthesia each year. During pregnancy, general anesthetics may affect brain development of the fetus. This study aimed to investigate safe dosage range of isoflurane. Methods Forty-eight SpragueDawley (SD) pregnant rats were randomly divided into 3 groups and inhaled 1.3% isoflurane (the Iso1 group), 2.0% isoflurane (the Iso2 group) and 50% O2 alone (the control group) for 3 h, respectively. Their offsprings were subjected to Morris water maze at day 28 and day 90 after birth to evaluate learning and memory. The expression of cAMP-response element binding protein (CREB) and phosphorylated cAMP-response element binding protein (p-CREB) was detected in the hippocampus dentate gyrus. Results Less offsprings of Iso2 group were able to cross the platform than that of the control group (P < 0.05). Accordingly, the Iso2 offsprings expressed p-CREB mainly in the subgranular zone in contrast to the whole granular cell layer of hippocampus dentate gyrus as detected in the Iso1 and control offsprings; the expression level of pCREB was also lower in the Iso2 than Iso1 or control offsprings (P < 0.05). Conclusion Inhalation of isoflurane at 1.3% during pregnancy has no significant influence on learning and memory of the offspring; exposure to isoflurane at 2.0% causes damage to spatial memory associated with inhibition of CREB phosphorylation in the granular cell layer of hippocampus dentate gyrus. |
| first_indexed | 2024-12-11T18:23:36Z |
| format | Article |
| id | doaj.art-96edc6d85922487dbc35879ddd12234a |
| institution | Directory Open Access Journal |
| issn | 1471-2253 |
| language | English |
| last_indexed | 2024-12-11T18:23:36Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Anesthesiology |
| spelling | doaj.art-96edc6d85922487dbc35879ddd12234a2022-12-22T00:55:11ZengBMCBMC Anesthesiology1471-22532018-01-011811710.1186/s12871-018-0471-2Influence of isoflurane exposure in pregnant rats on the learning and memory of offspringsWei Huang0Yunxia Dong1Guangyi Zhao2Yuan Wang3Jingjing Jiang4Ping Zhao5Department of Anesthesiology, Shengjing Hospital of China Medical UniversityDepartment of Anesthesiology, Shengjing Hospital of China Medical UniversityDepartment of Anesthesiology, Shengjing Hospital of China Medical UniversityDepartment of Anesthesiology, Shengjing Hospital of China Medical UniversityDepartment of Anesthesiology, Shengjing Hospital of China Medical UniversityDepartment of Anesthesiology, Shengjing Hospital of China Medical UniversityAbstract Background About 2% of pregnant women receive non-obstetric surgery under general anesthesia each year. During pregnancy, general anesthetics may affect brain development of the fetus. This study aimed to investigate safe dosage range of isoflurane. Methods Forty-eight SpragueDawley (SD) pregnant rats were randomly divided into 3 groups and inhaled 1.3% isoflurane (the Iso1 group), 2.0% isoflurane (the Iso2 group) and 50% O2 alone (the control group) for 3 h, respectively. Their offsprings were subjected to Morris water maze at day 28 and day 90 after birth to evaluate learning and memory. The expression of cAMP-response element binding protein (CREB) and phosphorylated cAMP-response element binding protein (p-CREB) was detected in the hippocampus dentate gyrus. Results Less offsprings of Iso2 group were able to cross the platform than that of the control group (P < 0.05). Accordingly, the Iso2 offsprings expressed p-CREB mainly in the subgranular zone in contrast to the whole granular cell layer of hippocampus dentate gyrus as detected in the Iso1 and control offsprings; the expression level of pCREB was also lower in the Iso2 than Iso1 or control offsprings (P < 0.05). Conclusion Inhalation of isoflurane at 1.3% during pregnancy has no significant influence on learning and memory of the offspring; exposure to isoflurane at 2.0% causes damage to spatial memory associated with inhibition of CREB phosphorylation in the granular cell layer of hippocampus dentate gyrus.http://link.springer.com/article/10.1186/s12871-018-0471-2IsofluraneCREBPregnancyMemoryOffsprings |
| spellingShingle | Wei Huang Yunxia Dong Guangyi Zhao Yuan Wang Jingjing Jiang Ping Zhao Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings BMC Anesthesiology Isoflurane CREB Pregnancy Memory Offsprings |
| title | Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings |
| title_full | Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings |
| title_fullStr | Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings |
| title_full_unstemmed | Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings |
| title_short | Influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings |
| title_sort | influence of isoflurane exposure in pregnant rats on the learning and memory of offsprings |
| topic | Isoflurane CREB Pregnancy Memory Offsprings |
| url | http://link.springer.com/article/10.1186/s12871-018-0471-2 |
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