Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia.
Phytochemical analysis of the dichloromethane:methanol (1:1) extract of root parts of Prangos pabularia led to the isolation of twelve cytotoxic constituents, viz., 6-hydroxycoumarin (1), 7-hydroxycoumarin (2), heraclenol-glycoside (3), xanthotoxol (4), heraclenol (5), oxypeucedanin hydrate (6), 8-(...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4196845?pdf=render |
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| author | Saleem Farooq Shakeel-u-Rehman Nisar Ahmad Dangroo Dev Priya Javid Ahmad Banday Pyare Lal Sangwan Mushtaq Ahmad Qurishi Surrinder Koul Ajit Kumar Saxena |
| author_facet | Saleem Farooq Shakeel-u-Rehman Nisar Ahmad Dangroo Dev Priya Javid Ahmad Banday Pyare Lal Sangwan Mushtaq Ahmad Qurishi Surrinder Koul Ajit Kumar Saxena |
| author_sort | Saleem Farooq |
| collection | DOAJ |
| description | Phytochemical analysis of the dichloromethane:methanol (1:1) extract of root parts of Prangos pabularia led to the isolation of twelve cytotoxic constituents, viz., 6-hydroxycoumarin (1), 7-hydroxycoumarin (2), heraclenol-glycoside (3), xanthotoxol (4), heraclenol (5), oxypeucedanin hydrate (6), 8-((3,3-dimethyloxiran-2-yl)methyl)-7-methoxy-2H-chromen-2-one (7), oxypeucedanin hydrate monoacetate (8), xanthotoxin (9), 4-((2-hydroxy-3-methylbut-3-en-1-yl)oxy)-7H-furo[3,2-g]chromen-7-one (10), imperatorin (11) and osthol (12). The isolates were identified using spectral techniques in the light of literature. 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity screening of the isolated constituents was carried out against six human cancer cell lines including lung (A549 and NCI-H322), epidermoid carcinoma (A431), melanoma (A375), prostate (PC-3) and Colon (HCT-116) cell lines. Osthol (12) exhibited the highest cytotoxicity with IC50 values of 3.2, 6.2, 10.9, 14.5, 24.8, and 30.2 µM against epidermoid carcinoma (A431), melanoma (A375), lung (NCI-H322), lung (A549), prostate (PC-3) and colon (HCT-116) cell lines respectively. Epidermoid carcinoma cell line A431 was sensitive to most of the compounds followed by lung (A549) cancer cell line. Finally a simple and reliable HPLC method was developed (RP-HPLC-DAD) and validated for the simultaneous quantification of these cytotoxic constituents in Prangos pabularia. The extract was analyzed using a reversed-phase Agilent ZORBAX eclipse plus column C18 (4.6×250 mm, 5 µm) at 250 nm wavelength using a gradient water-methanol solvent system at a flow rate of 0.8 ml/min. The RP-HPLC method is validated in terms of recovery, linearity, accuracy and precision (intra and inter-day validation). This method, because of shorter analysis time, makes it valuable for the commercial quality control of Prangos pabularia extracts and its future pharmaceutical preparations. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T21:02:10Z |
| publishDate | 2014-01-01 |
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| spelling | doaj.art-96ee2bb0b66d4e85aedac8d35248bbe42022-12-22T00:50:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10871310.1371/journal.pone.0108713Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia.Saleem FarooqShakeel-u-RehmanNisar Ahmad DangrooDev PriyaJavid Ahmad BandayPyare Lal SangwanMushtaq Ahmad QurishiSurrinder KoulAjit Kumar SaxenaPhytochemical analysis of the dichloromethane:methanol (1:1) extract of root parts of Prangos pabularia led to the isolation of twelve cytotoxic constituents, viz., 6-hydroxycoumarin (1), 7-hydroxycoumarin (2), heraclenol-glycoside (3), xanthotoxol (4), heraclenol (5), oxypeucedanin hydrate (6), 8-((3,3-dimethyloxiran-2-yl)methyl)-7-methoxy-2H-chromen-2-one (7), oxypeucedanin hydrate monoacetate (8), xanthotoxin (9), 4-((2-hydroxy-3-methylbut-3-en-1-yl)oxy)-7H-furo[3,2-g]chromen-7-one (10), imperatorin (11) and osthol (12). The isolates were identified using spectral techniques in the light of literature. 3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity screening of the isolated constituents was carried out against six human cancer cell lines including lung (A549 and NCI-H322), epidermoid carcinoma (A431), melanoma (A375), prostate (PC-3) and Colon (HCT-116) cell lines. Osthol (12) exhibited the highest cytotoxicity with IC50 values of 3.2, 6.2, 10.9, 14.5, 24.8, and 30.2 µM against epidermoid carcinoma (A431), melanoma (A375), lung (NCI-H322), lung (A549), prostate (PC-3) and colon (HCT-116) cell lines respectively. Epidermoid carcinoma cell line A431 was sensitive to most of the compounds followed by lung (A549) cancer cell line. Finally a simple and reliable HPLC method was developed (RP-HPLC-DAD) and validated for the simultaneous quantification of these cytotoxic constituents in Prangos pabularia. The extract was analyzed using a reversed-phase Agilent ZORBAX eclipse plus column C18 (4.6×250 mm, 5 µm) at 250 nm wavelength using a gradient water-methanol solvent system at a flow rate of 0.8 ml/min. The RP-HPLC method is validated in terms of recovery, linearity, accuracy and precision (intra and inter-day validation). This method, because of shorter analysis time, makes it valuable for the commercial quality control of Prangos pabularia extracts and its future pharmaceutical preparations.http://europepmc.org/articles/PMC4196845?pdf=render |
| spellingShingle | Saleem Farooq Shakeel-u-Rehman Nisar Ahmad Dangroo Dev Priya Javid Ahmad Banday Pyare Lal Sangwan Mushtaq Ahmad Qurishi Surrinder Koul Ajit Kumar Saxena Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia. PLoS ONE |
| title | Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia. |
| title_full | Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia. |
| title_fullStr | Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia. |
| title_full_unstemmed | Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia. |
| title_short | Isolation, cytotoxicity evaluation and HPLC-quantification of the chemical constituents from Prangos pabularia. |
| title_sort | isolation cytotoxicity evaluation and hplc quantification of the chemical constituents from prangos pabularia |
| url | http://europepmc.org/articles/PMC4196845?pdf=render |
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