Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique
Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade de Medicina Veterinária do Estado do Rio de Janeiro
2021-10-01
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| Series: | Brazilian Journal of Veterinary Medicine |
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| Online Access: | https://rbmv.org/BJVM/article/view/1198 |
| _version_ | 1819334296364646400 |
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| author | Mariana Costa Fausto Luís Henrique Gouvêa Saraiva Guilherme Costa Fausto Lorendane Millena de Carvalho Fabrício Luciani Valente Marisa Caixeta Valadão Artur Kanadani Campos Jackson Victor de Araújo |
| author_facet | Mariana Costa Fausto Luís Henrique Gouvêa Saraiva Guilherme Costa Fausto Lorendane Millena de Carvalho Fabrício Luciani Valente Marisa Caixeta Valadão Artur Kanadani Campos Jackson Victor de Araújo |
| author_sort | Mariana Costa Fausto |
| collection | DOAJ |
| description | Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs. |
| first_indexed | 2024-12-24T14:59:51Z |
| format | Article |
| id | doaj.art-96ee44d9063941119306a8811255bbfa |
| institution | Directory Open Access Journal |
| issn | 0100-2430 2527-2179 |
| language | English |
| last_indexed | 2024-12-24T14:59:51Z |
| publishDate | 2021-10-01 |
| publisher | Sociedade de Medicina Veterinária do Estado do Rio de Janeiro |
| record_format | Article |
| series | Brazilian Journal of Veterinary Medicine |
| spelling | doaj.art-96ee44d9063941119306a8811255bbfa2022-12-21T16:50:44ZengSociedade de Medicina Veterinária do Estado do Rio de JaneiroBrazilian Journal of Veterinary Medicine0100-24302527-21792021-10-01431e002221e00222110.29374/2527-2179.bjvm0022211069Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster techniqueMariana Costa Fausto0Luís Henrique Gouvêa Saraiva1Guilherme Costa Fausto2Lorendane Millena de Carvalho3Fabrício Luciani Valente4Marisa Caixeta Valadão5Artur Kanadani Campos6Jackson Victor de Araújo7Veterinarian, DSc. Programa de Pós-Graduação em Medicina Veterinária (PPGMV), Laboratório de Parasitologia e Doenças Parasitárias, Departamento de Veterinária (DVT), Universidade Federal de Viçosa (UFV). Campus Viçosa, MG, Brazil and Faculdade de Ciências Biológicas e da Saúde, Univiçosa, Viçosa, MG, Brazil. Undergraduate in Veterinary Medicine, Faculdade de Ciências Biológicas e da Saúde, Univiçosa, Viçosa, MG, Brazil. Veterinarian, DSc. PPGMV, Laboratório de Parasitologia e Doenças Parasitárias, DVT, UFV. Campus Viçosa, MG, Brazil. / Veterinarian, DSc., Faculdade de Ciências Biológicas e da Saúde, Univiçosa, Viçosa, MG, Brazil.Veterinarian, DSc. PPGMV, Laboratório de Parasitologia e Doenças Parasitárias, DVT, UFV. Campus Viçosa, MG, Brazil. Veterinarian, DSc., Laboratório de Parasitologia e Doenças Parasitárias, DVT, UFV, Campus Viçosa, MG, Brazil. Veterinarian, Laboratório de Parasitologia e Doenças Parasitárias, DVT, UFV, Campus Viçosa, MG, Brazil.Veterinarian, DSc., Laboratório de Parasitologia e Doenças Parasitárias, DVT, UFV, Campus Viçosa, MG, Brazil.Veterinarian, DSc., Laboratório de Parasitologia e Doenças Parasitárias, DVT, UFV, Campus Viçosa, MG, Brazil.Parasitic diseases are responsible for economic losses in the pig production industry. It is necessary to monitor the parasite load through stool ova and parasite analysis to avoid losses to the producer. Among analytical tests, the McMaster technique is the most widely used method; however, several variations of this method have been described, and, therefore, there is a need for standardization. The aim of this study was to evaluate the use of different saturated solutions prepared from four different salts (NaCl, MgSO4, NaNO3, ZnSO4), a sugar solution, and Polysorbate Tween 80, to perform the McMaster technique. Pig feces containing Ascaris suum eggs were homogenized in Griffin beakers and divided into 2g aliquots for the exams with different solutions. All solutions evaluated were able to float A. suum eggs. However, eggs per gram of feces (EPG) values using the sodium nitrate solution were higher (p < 0.05) than for the other solutions. Magnesium sulfate and sodium chloride solutions provided intermediate values for EPG. Lower values were obtained with zinc sulfate and sugar solutions. Polysorbate Tween 80 was added to the sodium nitrate solution at a concentration of 0.2%, and this proved to be more efficient (p < 0.05) in flotation of the eggs.https://rbmv.org/BJVM/article/view/1198parasitological techniques, sodium nitrate, tween 80. |
| spellingShingle | Mariana Costa Fausto Luís Henrique Gouvêa Saraiva Guilherme Costa Fausto Lorendane Millena de Carvalho Fabrício Luciani Valente Marisa Caixeta Valadão Artur Kanadani Campos Jackson Victor de Araújo Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique Brazilian Journal of Veterinary Medicine parasitological techniques, sodium nitrate, tween 80. |
| title | Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
| title_full | Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
| title_fullStr | Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
| title_full_unstemmed | Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
| title_short | Flotation solution influence on the quantification of Ascaris suum eggs in pig feces using McMaster technique |
| title_sort | flotation solution influence on the quantification of ascaris suum eggs in pig feces using mcmaster technique |
| topic | parasitological techniques, sodium nitrate, tween 80. |
| url | https://rbmv.org/BJVM/article/view/1198 |
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