Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy
The outer blood-retina barrier (oBRB), crucial for the survival and the proper functioning of the overlying retinal layers, is disrupted in numerous diseases affecting the retina, leading to the loss of the photoreceptors and ultimately of vision. To study the oBRB and/or its degeneration, many in v...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-01-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X23002426 |
| _version_ | 1797744011388125184 |
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| author | Chloé Dujardin Walter Habeler Christelle Monville Didier Letourneur Teresa Simon-Yarza |
| author_facet | Chloé Dujardin Walter Habeler Christelle Monville Didier Letourneur Teresa Simon-Yarza |
| author_sort | Chloé Dujardin |
| collection | DOAJ |
| description | The outer blood-retina barrier (oBRB), crucial for the survival and the proper functioning of the overlying retinal layers, is disrupted in numerous diseases affecting the retina, leading to the loss of the photoreceptors and ultimately of vision. To study the oBRB and/or its degeneration, many in vitro oBRB models have been developed, notably to investigate potential therapeutic strategies against retinal diseases. Indeed, to this day, most of these pathologies are untreatable, especially once the first signs of degeneration are observed. To cure those patients, a current strategy is to cultivate in vitro a mature oBRB epithelium on a custom membrane that is further implanted to replace the damaged native tissue. After a description of the oBRB and the related diseases, this review presents an overview of the oBRB models, from the simplest to the most complex. Then, we propose a discussion over the used cell types, for their relevance to study or treat the oBRB. Models designed for in vitro applications are then examined, by paying particular attention to the design evolution in the last years, the development of pathological models and the benefits of co-culture models, including both the retinal pigment epithelium and the choroid. Lastly, this review focuses on the models developed for in vivo implantation, with special emphasis on the choice of the material, its processing and its characterization, before discussing the reported pre-clinical and clinical trials. |
| first_indexed | 2024-03-12T15:03:35Z |
| format | Article |
| id | doaj.art-96ee66fbb7514119b87c4dc2e65c39ec |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-03-12T15:03:35Z |
| publishDate | 2024-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-96ee66fbb7514119b87c4dc2e65c39ec2023-08-13T04:54:31ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2024-01-0131151177Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapyChloé Dujardin0Walter Habeler1Christelle Monville2Didier Letourneur3Teresa Simon-Yarza4Université Paris Cité, Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science (LVTS) INSERM-U1148, 75018 Paris, France; Corresponding author.INSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100, Corbeil-Essonnes, France; U861, I-Stem, AFM, Université Paris-Saclay, Université D’Evry, 91100, Corbeil-Essonnes, France; CECS, Centre D’étude des Cellules Souches, 91100, Corbeil-Essonnes, FranceINSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100, Corbeil-Essonnes, France; U861, I-Stem, AFM, Université Paris-Saclay, Université D’Evry, 91100, Corbeil-Essonnes, FranceUniversité Paris Cité, Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science (LVTS) INSERM-U1148, 75018 Paris, FranceUniversité Paris Cité, Université Sorbonne Paris Nord, Laboratory for Vascular Translational Science (LVTS) INSERM-U1148, 75018 Paris, France; Corresponding author.The outer blood-retina barrier (oBRB), crucial for the survival and the proper functioning of the overlying retinal layers, is disrupted in numerous diseases affecting the retina, leading to the loss of the photoreceptors and ultimately of vision. To study the oBRB and/or its degeneration, many in vitro oBRB models have been developed, notably to investigate potential therapeutic strategies against retinal diseases. Indeed, to this day, most of these pathologies are untreatable, especially once the first signs of degeneration are observed. To cure those patients, a current strategy is to cultivate in vitro a mature oBRB epithelium on a custom membrane that is further implanted to replace the damaged native tissue. After a description of the oBRB and the related diseases, this review presents an overview of the oBRB models, from the simplest to the most complex. Then, we propose a discussion over the used cell types, for their relevance to study or treat the oBRB. Models designed for in vitro applications are then examined, by paying particular attention to the design evolution in the last years, the development of pathological models and the benefits of co-culture models, including both the retinal pigment epithelium and the choroid. Lastly, this review focuses on the models developed for in vivo implantation, with special emphasis on the choice of the material, its processing and its characterization, before discussing the reported pre-clinical and clinical trials.http://www.sciencedirect.com/science/article/pii/S2452199X23002426Outer-blood retina barrierTissue engineering and cellular therapyIn-vitro disease modelling3D complex models |
| spellingShingle | Chloé Dujardin Walter Habeler Christelle Monville Didier Letourneur Teresa Simon-Yarza Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy Bioactive Materials Outer-blood retina barrier Tissue engineering and cellular therapy In-vitro disease modelling 3D complex models |
| title | Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy |
| title_full | Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy |
| title_fullStr | Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy |
| title_full_unstemmed | Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy |
| title_short | Advances in the engineering of the outer blood-retina barrier: From in-vitro modelling to cellular therapy |
| title_sort | advances in the engineering of the outer blood retina barrier from in vitro modelling to cellular therapy |
| topic | Outer-blood retina barrier Tissue engineering and cellular therapy In-vitro disease modelling 3D complex models |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X23002426 |
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