Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” Trait
In this study, a novel T1RS.1BL translocation line RT843-5 was selected from a cross between wheat Mianyang11 (MY11) and Weining rye. The results of MC-FISH, PCR, and A-PAGE showed that RT843-5 contained two intact T1RS.1BL translocation chromosomes. RT843-5 showed resistance to the most virulent an...
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/9/4626 |
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| author | Zhi Li Qing Jiang Tao Fan Liqi Zhao Zhenglong Ren Feiquan Tan Peigao Luo Tianheng Ren |
| author_facet | Zhi Li Qing Jiang Tao Fan Liqi Zhao Zhenglong Ren Feiquan Tan Peigao Luo Tianheng Ren |
| author_sort | Zhi Li |
| collection | DOAJ |
| description | In this study, a novel T1RS.1BL translocation line RT843-5 was selected from a cross between wheat Mianyang11 (MY11) and Weining rye. The results of MC-FISH, PCR, and A-PAGE showed that RT843-5 contained two intact T1RS.1BL translocation chromosomes. RT843-5 showed resistance to the most virulent and frequently occurring stripe rust races/isolates. Additionally, RT843-5 showed resistance in the field in locations where stripe rust outbreaks have been the most severe in China. Genetic analysis indicated one new gene for stripe rust resistance, located on 1RS of RT843-5, which was tentatively named <i>YrRt843</i>. Furthermore, the chlorophyll content, the activities of catalase (CAT), and superoxide dismutase (SOD), and the net photosynthetic rate (Pn) of RT843-5 were significantly higher than those in its wheat parent MY11, whereas malondialdehyde (MDA) accumulation was significantly lower after anthesis in RT843-5 compared to in MY11. RT843-5 had a significantly higher 1000-kernel weight and yield than MY11. The results indicated that RT843-5 exhibited functional stay-green traits after anthesis, that delayed the senescence process in wheat leaves during the filling stage and had positive effects on grain yield. The present study indicated that Weining rye may carry untapped variations as a potential source of resistance, and that RT843-5 could be an important material for wheat breeding programs in the future. |
| first_indexed | 2024-03-10T04:07:23Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:07:23Z |
| publishDate | 2022-04-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-96f18602cec440749dbbf9cb126f51192023-11-23T08:19:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239462610.3390/ijms23094626Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” TraitZhi Li0Qing Jiang1Tao Fan2Liqi Zhao3Zhenglong Ren4Feiquan Tan5Peigao Luo6Tianheng Ren7College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaCollege of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, ChinaIn this study, a novel T1RS.1BL translocation line RT843-5 was selected from a cross between wheat Mianyang11 (MY11) and Weining rye. The results of MC-FISH, PCR, and A-PAGE showed that RT843-5 contained two intact T1RS.1BL translocation chromosomes. RT843-5 showed resistance to the most virulent and frequently occurring stripe rust races/isolates. Additionally, RT843-5 showed resistance in the field in locations where stripe rust outbreaks have been the most severe in China. Genetic analysis indicated one new gene for stripe rust resistance, located on 1RS of RT843-5, which was tentatively named <i>YrRt843</i>. Furthermore, the chlorophyll content, the activities of catalase (CAT), and superoxide dismutase (SOD), and the net photosynthetic rate (Pn) of RT843-5 were significantly higher than those in its wheat parent MY11, whereas malondialdehyde (MDA) accumulation was significantly lower after anthesis in RT843-5 compared to in MY11. RT843-5 had a significantly higher 1000-kernel weight and yield than MY11. The results indicated that RT843-5 exhibited functional stay-green traits after anthesis, that delayed the senescence process in wheat leaves during the filling stage and had positive effects on grain yield. The present study indicated that Weining rye may carry untapped variations as a potential source of resistance, and that RT843-5 could be an important material for wheat breeding programs in the future.https://www.mdpi.com/1422-0067/23/9/4626wheatchromosome translocationresistanceleaf senescenceWeining ryeFISH |
| spellingShingle | Zhi Li Qing Jiang Tao Fan Liqi Zhao Zhenglong Ren Feiquan Tan Peigao Luo Tianheng Ren Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” Trait International Journal of Molecular Sciences wheat chromosome translocation resistance leaf senescence Weining rye FISH |
| title | Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” Trait |
| title_full | Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” Trait |
| title_fullStr | Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” Trait |
| title_full_unstemmed | Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” Trait |
| title_short | Molecular Cytogenetic and Physiological Characterization of a Novel Wheat-Rye T1RS.1BL Translocation Line from <i>Secale cereal</i> L. Weining with Resistance to Stripe Rust and Functional “Stay Green” Trait |
| title_sort | molecular cytogenetic and physiological characterization of a novel wheat rye t1rs 1bl translocation line from i secale cereal i l weining with resistance to stripe rust and functional stay green trait |
| topic | wheat chromosome translocation resistance leaf senescence Weining rye FISH |
| url | https://www.mdpi.com/1422-0067/23/9/4626 |
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