Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense
Background: Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxic to the environment and could induce the resistance to pathogens in plants. In this work, we...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Journal of Ginseng Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1226845323001112 |
| _version_ | 1797598030066614272 |
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| author | Shuhan Zhang Junyou Han Ning Liu Jingyuan Sun Huchen Chen Jinglin Xia Huiyan Ju Shouan Liu |
| author_facet | Shuhan Zhang Junyou Han Ning Liu Jingyuan Sun Huchen Chen Jinglin Xia Huiyan Ju Shouan Liu |
| author_sort | Shuhan Zhang |
| collection | DOAJ |
| description | Background: Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxic to the environment and could induce the resistance to pathogens in plants. In this work, we try to understand if ginseng defense to B. cinerea could be induced by fungal hypovirulent strain △BcSpd1. BcSpd1 encodes Zn(II)2Cys6 transcription factor which regulates fungal pathogenicity and we recently reported △BcSpd1 mutants reduced fungal virulence. Methods: We performed transcriptomic analysis of the host to investigate the induced defense response of ginseng treated by B. cinerea △BcSpd1. The metabolites in ginseng flavonoids pathway were determined by UPLC-ESI-MS/MS and the antifungal activates were then performed. Results: We found that △BcSpd1 enhanced the ginseng defense response when applied to healthy ginseng leaves and further changed the metabolism of flavonoids. Compared with untreated plants, the application of △BcSpd1 on ginseng leaves significantly increased the accumulation of p-coumaric acid and myricetin, which could inhibit the fungal growth. Conclusion: B. cinerea △BcSpd1 could effectively induce the medicinal plant defense and is referred to as the biological control agent in ginseng disease management. |
| first_indexed | 2024-03-11T03:13:38Z |
| format | Article |
| id | doaj.art-96f4e94212ba45e1af431c831df06cee |
| institution | Directory Open Access Journal |
| issn | 1226-8453 |
| language | English |
| last_indexed | 2024-03-11T03:13:38Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ginseng Research |
| spelling | doaj.art-96f4e94212ba45e1af431c831df06cee2023-11-18T04:28:20ZengElsevierJournal of Ginseng Research1226-84532023-11-01476773783Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defenseShuhan Zhang0Junyou Han1Ning Liu2Jingyuan Sun3Huchen Chen4Jinglin Xia5Huiyan Ju6Shouan Liu7Laboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, ChinaLaboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, ChinaInstitute of Special Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, ChinaLaboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, ChinaLaboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, ChinaLaboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, ChinaLaboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, ChinaLaboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, China; Corresponding author. Laboratory of Tea and Medicinal Plant Pathology, Jilin University, Changchun, 130062, China.Background: Gray mold, caused by Botrytis cinerea, is one of the major fungal diseases in agriculture. Biological methods are preferred over chemical fungicides to control gray mold since they are less toxic to the environment and could induce the resistance to pathogens in plants. In this work, we try to understand if ginseng defense to B. cinerea could be induced by fungal hypovirulent strain △BcSpd1. BcSpd1 encodes Zn(II)2Cys6 transcription factor which regulates fungal pathogenicity and we recently reported △BcSpd1 mutants reduced fungal virulence. Methods: We performed transcriptomic analysis of the host to investigate the induced defense response of ginseng treated by B. cinerea △BcSpd1. The metabolites in ginseng flavonoids pathway were determined by UPLC-ESI-MS/MS and the antifungal activates were then performed. Results: We found that △BcSpd1 enhanced the ginseng defense response when applied to healthy ginseng leaves and further changed the metabolism of flavonoids. Compared with untreated plants, the application of △BcSpd1 on ginseng leaves significantly increased the accumulation of p-coumaric acid and myricetin, which could inhibit the fungal growth. Conclusion: B. cinerea △BcSpd1 could effectively induce the medicinal plant defense and is referred to as the biological control agent in ginseng disease management.http://www.sciencedirect.com/science/article/pii/S1226845323001112Botrytis cinereaPanax ginsengp-coumaric acidmyricetintranscriptome |
| spellingShingle | Shuhan Zhang Junyou Han Ning Liu Jingyuan Sun Huchen Chen Jinglin Xia Huiyan Ju Shouan Liu Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense Journal of Ginseng Research Botrytis cinerea Panax ginseng p-coumaric acid myricetin transcriptome |
| title | Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense |
| title_full | Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense |
| title_fullStr | Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense |
| title_full_unstemmed | Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense |
| title_short | Botrytis cinerea hypovirulent strain △BcSpd1 induced Panax ginseng defense |
| title_sort | botrytis cinerea hypovirulent strain △bcspd1 induced panax ginseng defense |
| topic | Botrytis cinerea Panax ginseng p-coumaric acid myricetin transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S1226845323001112 |
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