Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in Mice
The consumption of large amounts of dietary fats and pregnancy are independent factors that can promote changes in gut permeability and the gut microbiome landscape. However, there is limited evidence regarding the impact of pregnancy on the regulation of such parameters in females fed a high-fat di...
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MDPI AG
2023-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/24/5041 |
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| author | Caio F. Biolcatti Vanessa C. Bobbo Carina Solon Joseane Morari Roberta Haddad-Tovolli Eliana P. Araujo Marcela R. Simoes Licio A. Velloso |
| author_facet | Caio F. Biolcatti Vanessa C. Bobbo Carina Solon Joseane Morari Roberta Haddad-Tovolli Eliana P. Araujo Marcela R. Simoes Licio A. Velloso |
| author_sort | Caio F. Biolcatti |
| collection | DOAJ |
| description | The consumption of large amounts of dietary fats and pregnancy are independent factors that can promote changes in gut permeability and the gut microbiome landscape. However, there is limited evidence regarding the impact of pregnancy on the regulation of such parameters in females fed a high-fat diet. Here, gut permeability and microbiome landscape were evaluated in a mouse model of diet-induced obesity in pregnancy. The results show that pregnancy protected against the harmful effects of the consumption of a high-fat diet as a disruptor of gut permeability; thus, there was a two-fold reduction in FITC–dextran passage to the bloodstream compared to non-pregnant mice fed a high-fat diet (<i>p</i> < 0.01). This was accompanied by an increased expression of gut barrier-related transcripts, particularly in the ileum. In addition, the beneficial effect of pregnancy on female mice fed the high-fat diet was accompanied by a reduced presence of bacteria belonging to the genus <i>Clostridia</i>, and by increased <i>Lactobacillus murinus</i> in the gut (<i>p</i> < 0.05). Thus, this study advances the understanding of how pregnancy can act during a short window of time, protecting against the harmful effects of the consumption of a high-fat diet by promoting an increased expression of transcripts encoding proteins involved in the regulation of gut permeability, particularly in the ileum, and promoting changes in the gut microbiome. |
| first_indexed | 2024-03-08T20:28:12Z |
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| id | doaj.art-96f57e26e4e34dfa8d90badd118edc21 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-08T20:28:12Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-96f57e26e4e34dfa8d90badd118edc212023-12-22T14:29:55ZengMDPI AGNutrients2072-66432023-12-011524504110.3390/nu15245041Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in MiceCaio F. Biolcatti0Vanessa C. Bobbo1Carina Solon2Joseane Morari3Roberta Haddad-Tovolli4Eliana P. Araujo5Marcela R. Simoes6Licio A. Velloso7Laboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilLaboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilLaboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilLaboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilLaboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilLaboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilLaboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilLaboratory of Cell Signaling, Obesity and Comorbidities Research Center, University of Campinas, Campinas 13083-864, BrazilThe consumption of large amounts of dietary fats and pregnancy are independent factors that can promote changes in gut permeability and the gut microbiome landscape. However, there is limited evidence regarding the impact of pregnancy on the regulation of such parameters in females fed a high-fat diet. Here, gut permeability and microbiome landscape were evaluated in a mouse model of diet-induced obesity in pregnancy. The results show that pregnancy protected against the harmful effects of the consumption of a high-fat diet as a disruptor of gut permeability; thus, there was a two-fold reduction in FITC–dextran passage to the bloodstream compared to non-pregnant mice fed a high-fat diet (<i>p</i> < 0.01). This was accompanied by an increased expression of gut barrier-related transcripts, particularly in the ileum. In addition, the beneficial effect of pregnancy on female mice fed the high-fat diet was accompanied by a reduced presence of bacteria belonging to the genus <i>Clostridia</i>, and by increased <i>Lactobacillus murinus</i> in the gut (<i>p</i> < 0.05). Thus, this study advances the understanding of how pregnancy can act during a short window of time, protecting against the harmful effects of the consumption of a high-fat diet by promoting an increased expression of transcripts encoding proteins involved in the regulation of gut permeability, particularly in the ileum, and promoting changes in the gut microbiome.https://www.mdpi.com/2072-6643/15/24/5041obesityreproductionmetabolismfatty acidmicrobiota |
| spellingShingle | Caio F. Biolcatti Vanessa C. Bobbo Carina Solon Joseane Morari Roberta Haddad-Tovolli Eliana P. Araujo Marcela R. Simoes Licio A. Velloso Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in Mice Nutrients obesity reproduction metabolism fatty acid microbiota |
| title | Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in Mice |
| title_full | Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in Mice |
| title_fullStr | Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in Mice |
| title_full_unstemmed | Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in Mice |
| title_short | Pregnancy Protects against Abnormal Gut Permeability Promoted via the Consumption of a High-Fat Diet in Mice |
| title_sort | pregnancy protects against abnormal gut permeability promoted via the consumption of a high fat diet in mice |
| topic | obesity reproduction metabolism fatty acid microbiota |
| url | https://www.mdpi.com/2072-6643/15/24/5041 |
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