Hook2 contributes to aggresome formation
<p>Abstract</p> <p>Background</p> <p>Aggresomes are pericentrosomal accumulations of misfolded proteins, chaperones and proteasomes. Their positioning near the centrosome, like that of other organelles, requires active, microtubule-dependent transport. Linker proteins t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-05-01
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| Series: | BMC Cell Biology |
| Online Access: | http://www.biomedcentral.com/1471-2121/8/19 |
| _version_ | 1818564047242526720 |
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| author | Yu Michelle Didier Aaron Hall Branden Wigley W Christian Szebenyi Györgyi Thomas Philip Krämer Helmut |
| author_facet | Yu Michelle Didier Aaron Hall Branden Wigley W Christian Szebenyi Györgyi Thomas Philip Krämer Helmut |
| author_sort | Yu Michelle |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Aggresomes are pericentrosomal accumulations of misfolded proteins, chaperones and proteasomes. Their positioning near the centrosome, like that of other organelles, requires active, microtubule-dependent transport. Linker proteins that can associate with the motor protein dynein, organelles, and microtubules are thought to contribute to the active maintenance of the juxtanuclear localization of many membrane bound organelles and aggresomes. Hook proteins have been proposed to serve as adaptors for the association of cargos with dynein for transport on microtubules. Hook2 was shown to localize to the centrosome, bind centriolin, and contribute to centrosomal function.</p> <p>Results</p> <p>Here we show that overexpression of hook2 promotes the accumulation of the cystic fibrosis transmembrane regulator in aggresomes without altering its biochemical properties or its steady state level. A dominant negatively acting form of hook2 that lacks the centriolin binding C-terminal inhibits aggresome formation.</p> <p>Conclusion</p> <p>We propose that hook2 contributes to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes.</p> |
| first_indexed | 2024-12-14T01:24:16Z |
| format | Article |
| id | doaj.art-96f74df542cb4528a411637d5295c361 |
| institution | Directory Open Access Journal |
| issn | 1471-2121 |
| language | English |
| last_indexed | 2024-12-14T01:24:16Z |
| publishDate | 2007-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cell Biology |
| spelling | doaj.art-96f74df542cb4528a411637d5295c3612022-12-21T23:22:16ZengBMCBMC Cell Biology1471-21212007-05-01811910.1186/1471-2121-8-19Hook2 contributes to aggresome formationYu MichelleDidier AaronHall BrandenWigley W ChristianSzebenyi GyörgyiThomas PhilipKrämer Helmut<p>Abstract</p> <p>Background</p> <p>Aggresomes are pericentrosomal accumulations of misfolded proteins, chaperones and proteasomes. Their positioning near the centrosome, like that of other organelles, requires active, microtubule-dependent transport. Linker proteins that can associate with the motor protein dynein, organelles, and microtubules are thought to contribute to the active maintenance of the juxtanuclear localization of many membrane bound organelles and aggresomes. Hook proteins have been proposed to serve as adaptors for the association of cargos with dynein for transport on microtubules. Hook2 was shown to localize to the centrosome, bind centriolin, and contribute to centrosomal function.</p> <p>Results</p> <p>Here we show that overexpression of hook2 promotes the accumulation of the cystic fibrosis transmembrane regulator in aggresomes without altering its biochemical properties or its steady state level. A dominant negatively acting form of hook2 that lacks the centriolin binding C-terminal inhibits aggresome formation.</p> <p>Conclusion</p> <p>We propose that hook2 contributes to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes.</p>http://www.biomedcentral.com/1471-2121/8/19 |
| spellingShingle | Yu Michelle Didier Aaron Hall Branden Wigley W Christian Szebenyi Györgyi Thomas Philip Krämer Helmut Hook2 contributes to aggresome formation BMC Cell Biology |
| title | Hook2 contributes to aggresome formation |
| title_full | Hook2 contributes to aggresome formation |
| title_fullStr | Hook2 contributes to aggresome formation |
| title_full_unstemmed | Hook2 contributes to aggresome formation |
| title_short | Hook2 contributes to aggresome formation |
| title_sort | hook2 contributes to aggresome formation |
| url | http://www.biomedcentral.com/1471-2121/8/19 |
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