Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase

Biochemical Characterization of Middle East Respiratory Syndrome Coronavirus Helicase

ABSTRACT Middle East respiratory syndrome coronavirus (MERS-CoV) helicase is a superfamily 1 helicase containing seven conserved motifs. We have cloned, expressed, and purified a Strep-fused recombinant MERS-CoV nonstructural protein 13 (M-nsp13) helicase. Characterization of its biochemical propert...

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Bibliographic Details
Main Authors: Adeyemi O. Adedeji, Hilary Lazarus
Format: Article
Language:English
Published: American Society for Microbiology 2016-10-01
Series:mSphere
Subjects:
ATP hydrolysis
DNA
RNA
coronavirus
enzyme kinetics
helicase
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00235-16

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