Human embryo twinning with proof of monozygocity
Objective: To prove monozygocity of human twin blastocysts derived from embryo splitting at the cleavage stage. Methods: Human triploid embryos at the 6–8 cell stage were split into twin embryos (D and R) and cultured in vitro to the blastocyst stage. Twin embryos were processed for nested multiplex...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2011-09-01
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| Series: | Middle East Fertility Society Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110569011000331 |
| _version_ | 1831728865088634880 |
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| author | Karl Illmensee Mike Levanduski Christopher Konialis Constantinos Pangalos Alexandros Vithoulkas Vasilios T. Goudas |
| author_facet | Karl Illmensee Mike Levanduski Christopher Konialis Constantinos Pangalos Alexandros Vithoulkas Vasilios T. Goudas |
| author_sort | Karl Illmensee |
| collection | DOAJ |
| description | Objective: To prove monozygocity of human twin blastocysts derived from embryo splitting at the cleavage stage.
Methods: Human triploid embryos at the 6–8 cell stage were split into twin embryos (D and R) and cultured in vitro to the blastocyst stage. Twin embryos were processed for nested multiplex PCR using fluorescently labeled primers of six selected polymorphic STR markers within the human HLA locus.
Results: A total of 11 pairs of twin embryos were analyzed with PCR technology. From five pairs of twin blastocysts, the fluorograms provided detectable STR profiles for identical peak position between the twin embryos.
Conclusion: This is the first report on twinned human embryos to prove monozygocity at the DNA level. Embryo splitting exhibits novel potential for future applications in assisted reproductive medicine. |
| first_indexed | 2024-12-21T06:58:27Z |
| format | Article |
| id | doaj.art-96fa6e6fd9d44f9bbeb3b18e20f38025 |
| institution | Directory Open Access Journal |
| issn | 1110-5690 |
| language | English |
| last_indexed | 2024-12-21T06:58:27Z |
| publishDate | 2011-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj.art-96fa6e6fd9d44f9bbeb3b18e20f380252022-12-21T19:12:17ZengSpringerOpenMiddle East Fertility Society Journal1110-56902011-09-0116321521910.1016/j.mefs.2011.03.004Human embryo twinning with proof of monozygocityKarl Illmensee0Mike Levanduski1Christopher Konialis2Constantinos Pangalos3Alexandros Vithoulkas4Vasilios T. Goudas5Genesis Fertility Center, Patra, GreeceEmbryoserv Corporation Ltd., New York, NY, USAInterGenetics-Diagnostic Genetic Center, Pireaus, GreeceInterGenetics-Diagnostic Genetic Center, Pireaus, GreeceGenesis Fertility Center, Patra, GreeceGenesis Fertility Center, Patra, GreeceObjective: To prove monozygocity of human twin blastocysts derived from embryo splitting at the cleavage stage. Methods: Human triploid embryos at the 6–8 cell stage were split into twin embryos (D and R) and cultured in vitro to the blastocyst stage. Twin embryos were processed for nested multiplex PCR using fluorescently labeled primers of six selected polymorphic STR markers within the human HLA locus. Results: A total of 11 pairs of twin embryos were analyzed with PCR technology. From five pairs of twin blastocysts, the fluorograms provided detectable STR profiles for identical peak position between the twin embryos. Conclusion: This is the first report on twinned human embryos to prove monozygocity at the DNA level. Embryo splitting exhibits novel potential for future applications in assisted reproductive medicine.http://www.sciencedirect.com/science/article/pii/S1110569011000331Assisted reproductive technology (ART)Human embryo splittingMonozygocityPCR and DNA technologySTR markers |
| spellingShingle | Karl Illmensee Mike Levanduski Christopher Konialis Constantinos Pangalos Alexandros Vithoulkas Vasilios T. Goudas Human embryo twinning with proof of monozygocity Middle East Fertility Society Journal Assisted reproductive technology (ART) Human embryo splitting Monozygocity PCR and DNA technology STR markers |
| title | Human embryo twinning with proof of monozygocity |
| title_full | Human embryo twinning with proof of monozygocity |
| title_fullStr | Human embryo twinning with proof of monozygocity |
| title_full_unstemmed | Human embryo twinning with proof of monozygocity |
| title_short | Human embryo twinning with proof of monozygocity |
| title_sort | human embryo twinning with proof of monozygocity |
| topic | Assisted reproductive technology (ART) Human embryo splitting Monozygocity PCR and DNA technology STR markers |
| url | http://www.sciencedirect.com/science/article/pii/S1110569011000331 |
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