Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicity
Pseudomonas aeruginosa is one of the most common pathogens isolated in clinical settings and produces a wide range of extracellular molecules that contributes to the virulence. Chemotherapy options to prevent and treat P. aeruginosa infections are limited because this pathogen is highly and innately...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517422000578 |
| _version_ | 1811178524542763008 |
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| author | Hidetada Hirakawa Ayuko Kimura Ayako Takita Sayaka Chihara Koichi Tanimoto Haruyoshi Tomita |
| author_facet | Hidetada Hirakawa Ayuko Kimura Ayako Takita Sayaka Chihara Koichi Tanimoto Haruyoshi Tomita |
| author_sort | Hidetada Hirakawa |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is one of the most common pathogens isolated in clinical settings and produces a wide range of extracellular molecules that contributes to the virulence. Chemotherapy options to prevent and treat P. aeruginosa infections are limited because this pathogen is highly and innately resistant to some classes of conventional drugs. Alternative methods to conquer P. aeruginosa, including multidrug resistant strains, are being investigated. This study showed that a macroporous magnesium oxide (MgO)-templated carbon material (MgOC150) attenuates the toxicity of this bacterium in human epithelial cells. A proteomic analysis revealed that MgOC150 adsorbs some extracellular proteases, including elastase (LasB) and alkaline protease (AprA), required for the virulence of P. aeruginosa, which decreases the accumulation of these molecules. MgOC150 also adsorbed pyocyanin, which is another molecule involved in its pathogenesis, but is a nonprotein small-sized molecule. These results suggest a potency of MgOC150 that suppresses the virulence of P. aeruginosa. MgOC150 has been used for industrial purposes, as an electrode catalyst and a bioelectrode and for enzyme immobilization. Thus, MgOC150 could be beneficial for developing novel anti-Pseudomonas therapy. |
| first_indexed | 2024-04-11T06:20:53Z |
| format | Article |
| id | doaj.art-96fba75503ee445fa47b1f34214f094c |
| institution | Directory Open Access Journal |
| issn | 2666-5174 |
| language | English |
| last_indexed | 2024-04-11T06:20:53Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj.art-96fba75503ee445fa47b1f34214f094c2022-12-22T04:40:36ZengElsevierCurrent Research in Microbial Sciences2666-51742022-01-013100160Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicityHidetada Hirakawa0Ayuko Kimura1Ayako Takita2Sayaka Chihara3Koichi Tanimoto4Haruyoshi Tomita5Department of Bacteriology, Gunma University, Graduate School of Medicine, 3-39-22 Showa-machi Maebashi, Gunma 371-8511, Japan; Corresponding author.Graduate School of Health Science, Gunma Paz University, Tonyamachi 1-7-1, Takasaki City, Gunma 370-0006, JapanDepartment of Bacteriology, Gunma University, Graduate School of Medicine, 3-39-22 Showa-machi Maebashi, Gunma 371-8511, JapanDepartment of Bacteriology, Gunma University, Graduate School of Medicine, 3-39-22 Showa-machi Maebashi, Gunma 371-8511, JapanLaboratory of Bacterial Drug Resistance, Gunma University, Graduate School of Medicine, 3-39-22 Showa-machi Maebashi, Gunma 371-8511Department of Bacteriology, Gunma University, Graduate School of Medicine, 3-39-22 Showa-machi Maebashi, Gunma 371-8511, Japan; Laboratory of Bacterial Drug Resistance, Gunma University, Graduate School of Medicine, 3-39-22 Showa-machi Maebashi, Gunma 371-8511Pseudomonas aeruginosa is one of the most common pathogens isolated in clinical settings and produces a wide range of extracellular molecules that contributes to the virulence. Chemotherapy options to prevent and treat P. aeruginosa infections are limited because this pathogen is highly and innately resistant to some classes of conventional drugs. Alternative methods to conquer P. aeruginosa, including multidrug resistant strains, are being investigated. This study showed that a macroporous magnesium oxide (MgO)-templated carbon material (MgOC150) attenuates the toxicity of this bacterium in human epithelial cells. A proteomic analysis revealed that MgOC150 adsorbs some extracellular proteases, including elastase (LasB) and alkaline protease (AprA), required for the virulence of P. aeruginosa, which decreases the accumulation of these molecules. MgOC150 also adsorbed pyocyanin, which is another molecule involved in its pathogenesis, but is a nonprotein small-sized molecule. These results suggest a potency of MgOC150 that suppresses the virulence of P. aeruginosa. MgOC150 has been used for industrial purposes, as an electrode catalyst and a bioelectrode and for enzyme immobilization. Thus, MgOC150 could be beneficial for developing novel anti-Pseudomonas therapy.http://www.sciencedirect.com/science/article/pii/S2666517422000578Antimicrobial resistance (AMR)VirulenceExtracellular proteasePorous carbonAdsorbentBacterial translocation |
| spellingShingle | Hidetada Hirakawa Ayuko Kimura Ayako Takita Sayaka Chihara Koichi Tanimoto Haruyoshi Tomita Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicity Current Research in Microbial Sciences Antimicrobial resistance (AMR) Virulence Extracellular protease Porous carbon Adsorbent Bacterial translocation |
| title | Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicity |
| title_full | Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicity |
| title_fullStr | Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicity |
| title_full_unstemmed | Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicity |
| title_short | Adsorption of extracellular proteases and pyocyanin produced by Pseudomonas aeruginosa using a macroporous magnesium oxide-templated carbon decreases cytotoxicity |
| title_sort | adsorption of extracellular proteases and pyocyanin produced by pseudomonas aeruginosa using a macroporous magnesium oxide templated carbon decreases cytotoxicity |
| topic | Antimicrobial resistance (AMR) Virulence Extracellular protease Porous carbon Adsorbent Bacterial translocation |
| url | http://www.sciencedirect.com/science/article/pii/S2666517422000578 |
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