Pembuktian horizontal transfer of resistance genes melalui uji sensitivitas antibiotika pada bakteri genus Staphylococcus dari kasus bovine mastitis

The experiment was carried out to show the evidence of horizontal transfer of resistance genes by antibiotics sensitivity teston bacteria of genus of Staphylococcus from bovine mastitic cases. The first step of the experiment was to prepare pure culture ofStaphylococcus aureus and Coagulase Negative...

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Bibliographic Details
Main Author: Mustofa i Helmi Effend
Format: Article
Language:English
Published: Indonesian Biological Society 2012-02-01
Series:Journal of Biological Researches
Subjects:
Online Access:http://berkalahayati.org/journal/db30680a
Description
Summary:The experiment was carried out to show the evidence of horizontal transfer of resistance genes by antibiotics sensitivity teston bacteria of genus of Staphylococcus from bovine mastitic cases. The first step of the experiment was to prepare pure culture ofStaphylococcus aureus and Coagulase Negative Staphylococci. Milk samples were collected from mastitic cases at the afternoonmilking time. Preparation of pure culture of isolates were confirmed by MS agar, hemolytic activity, catalase and coagulase test.Both isolates were tested by using antibiotics sensitivity test. The result showed that the both isolates of Staphylococcus aureus andCoagulase Negative Staphylococci from Surabaya dairy herd were resistant against erythromycin antibiotic. Based on the result, itcan be concluded that there was horizontal transfer of resistance genes between both isolates from same udder of dairy cows.
ISSN:0852-6834
2337-389X