Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer
Computer-assisted sperm morphometry analysis has improved the assessment of sperm morphology, but the results depend on the use of adequate evaluation and staining procedures of spermatozoa from individual species. In this study, the morphological module of the Sperm Class Analyzer®was used for the...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2005-01-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200501-0003_effect-of-sample-size-and-staining-methods-on-stallion-sperm-morphometry-by-the-sperm-class-analyzer.php |
| _version_ | 1797897737551740928 |
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| author | M. Hidalgo I. Rodriguez J. Dorado J. Sanz C. Soler |
| author_facet | M. Hidalgo I. Rodriguez J. Dorado J. Sanz C. Soler |
| author_sort | M. Hidalgo |
| collection | DOAJ |
| description | Computer-assisted sperm morphometry analysis has improved the assessment of sperm morphology, but the results depend on the use of adequate evaluation and staining procedures of spermatozoa from individual species. In this study, the morphological module of the Sperm Class Analyzer®was used for the morphometric analysis of stallion sperm heads and midpieces. Semen samples were obtained from six fertile stallions in order to evaluate the influence of three staining procedures (Diff-Quik, Hemacolor and Harris' Haematoxylin) on the accuracy of image processing and sperm morphometry, and the effect of the sample size on sperm morphometric measurements. Harris' Haematoxylin was the staining technique of choice on the accuracy of the image processing with an optimum contrast of sperm cells with the surrounding background that allows an efficient boundary detection and segmentation which results in the highest proportion of sperm heads and midpieces assessed (80.47%). The results indicate that the staining methods affected significantly the sperm dimensions with increased values from Diff-Quik than Hemacolor and Harris' Haematoxylin respectively (Diff-Quik > Hemacolor > Harris' Haematoxylin). No differences in morphometric parameters were found when 100, 150, 175 or 200 spermatozoa were analysed. In conclusion, to obtain objective and accurate sperm morphometric measurements by the Sperm Class Analyzer® system in the stallion, it's recommended the analysis of 100 spermatozoa from slides which have been previously stained with Harris' Haematoxylin. |
| first_indexed | 2024-04-10T08:03:09Z |
| format | Article |
| id | doaj.art-96feac96e25a45bb8a4a3c8bc5829f49 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:03:09Z |
| publishDate | 2005-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-96feac96e25a45bb8a4a3c8bc5829f492023-02-23T03:49:16ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922005-01-01501243210.17221/5593-VETMEDvet-200501-0003Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class AnalyzerM. Hidalgo0I. Rodriguez1J. Dorado2J. Sanz3C. Soler4Reproduction and Obstetrics Unit, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, SpainReproduction and Obstetrics Unit, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, SpainReproduction and Obstetrics Unit, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, SpainReproduction and Obstetrics Unit, Department of Animal Medicine and Surgery, University of Cordoba, Cordoba, SpainPhysical Anthropology Unit, Department of Functional Biology and Physical Anthropology, University of Valencia, Valencia, SpainComputer-assisted sperm morphometry analysis has improved the assessment of sperm morphology, but the results depend on the use of adequate evaluation and staining procedures of spermatozoa from individual species. In this study, the morphological module of the Sperm Class Analyzer®was used for the morphometric analysis of stallion sperm heads and midpieces. Semen samples were obtained from six fertile stallions in order to evaluate the influence of three staining procedures (Diff-Quik, Hemacolor and Harris' Haematoxylin) on the accuracy of image processing and sperm morphometry, and the effect of the sample size on sperm morphometric measurements. Harris' Haematoxylin was the staining technique of choice on the accuracy of the image processing with an optimum contrast of sperm cells with the surrounding background that allows an efficient boundary detection and segmentation which results in the highest proportion of sperm heads and midpieces assessed (80.47%). The results indicate that the staining methods affected significantly the sperm dimensions with increased values from Diff-Quik than Hemacolor and Harris' Haematoxylin respectively (Diff-Quik > Hemacolor > Harris' Haematoxylin). No differences in morphometric parameters were found when 100, 150, 175 or 200 spermatozoa were analysed. In conclusion, to obtain objective and accurate sperm morphometric measurements by the Sperm Class Analyzer® system in the stallion, it's recommended the analysis of 100 spermatozoa from slides which have been previously stained with Harris' Haematoxylin.https://vetmed.agriculturejournals.cz/artkey/vet-200501-0003_effect-of-sample-size-and-staining-methods-on-stallion-sperm-morphometry-by-the-sperm-class-analyzer.phpasmasperm headmidpiecemorphometric anlaysisvalidation |
| spellingShingle | M. Hidalgo I. Rodriguez J. Dorado J. Sanz C. Soler Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer Veterinární Medicína asma sperm head midpiece morphometric anlaysis validation |
| title | Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer |
| title_full | Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer |
| title_fullStr | Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer |
| title_full_unstemmed | Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer |
| title_short | Effect of sample size and staining methods on stallion sperm morphometry by the Sperm Class Analyzer |
| title_sort | effect of sample size and staining methods on stallion sperm morphometry by the sperm class analyzer |
| topic | asma sperm head midpiece morphometric anlaysis validation |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200501-0003_effect-of-sample-size-and-staining-methods-on-stallion-sperm-morphometry-by-the-sperm-class-analyzer.php |
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