Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats
The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite...
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| Format: | Article |
| Language: | English |
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SciELO
2010-01-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016 |
| _version_ | 1819118561375813632 |
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| author | VM Costa H Langoni |
| author_facet | VM Costa H Langoni |
| author_sort | VM Costa |
| collection | DOAJ |
| description | The milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR. |
| first_indexed | 2024-12-22T05:50:50Z |
| format | Article |
| id | doaj.art-96ff6734f2834371a1fcd938282127f7 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-22T05:50:50Z |
| publishDate | 2010-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-96ff6734f2834371a1fcd938282127f72022-12-21T18:36:52ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992010-01-0116236837410.1590/S1678-91992010000200016Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female ratsVM CostaH LangoniThe milk of experimentally infected rats was investigated for the presence and possible transmission of Toxoplasma gondii. Wistar (Rattus norvegicus) female rats were divided into three groups and orally inoculated with bradyzoites. Polymerase chain reaction (PCR) was employed to detect the parasite in the milk. Transmission to the offspring was verified by indirect immunofluorescence antibody test (IFAT), modified agglutination test (MAT), bioassay tests and PCR. Rat milk samples were PCR-positive, pups were serum-reactive to T. gondii and tissue samples also presented positive DNA results through PCR.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016Toxoplasma gondiiparasite in milkfemale ratsPCRbioassay |
| spellingShingle | VM Costa H Langoni Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats Journal of Venomous Animals and Toxins including Tropical Diseases Toxoplasma gondii parasite in milk female rats PCR bioassay |
| title | Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats |
| title_full | Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats |
| title_fullStr | Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats |
| title_full_unstemmed | Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats |
| title_short | Detection of Toxoplasma gondii in the milk of experimentally infected Wistar female rats |
| title_sort | detection of toxoplasma gondii in the milk of experimentally infected wistar female rats |
| topic | Toxoplasma gondii parasite in milk female rats PCR bioassay |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000200016 |
| work_keys_str_mv | AT vmcosta detectionoftoxoplasmagondiiinthemilkofexperimentallyinfectedwistarfemalerats AT hlangoni detectionoftoxoplasmagondiiinthemilkofexperimentallyinfectedwistarfemalerats |