Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia
BackgroundTuberculosis (TB) is a leading cause of morbidity and mortality in Ethiopia. Investigation of the Mycobacterium tuberculosis complex (MTBC) species circulating in the Ethiopian population would contribute to the efforts made to control TB in the country. Therefore, this study was conducted...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2022.808626/full |
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| author | Bedru Hussien Aboma Zewude Aboma Zewude Biniam Wondale Awraris Hailu Gobena Ameni Gobena Ameni |
| author_facet | Bedru Hussien Aboma Zewude Aboma Zewude Biniam Wondale Awraris Hailu Gobena Ameni Gobena Ameni |
| author_sort | Bedru Hussien |
| collection | DOAJ |
| description | BackgroundTuberculosis (TB) is a leading cause of morbidity and mortality in Ethiopia. Investigation of the Mycobacterium tuberculosis complex (MTBC) species circulating in the Ethiopian population would contribute to the efforts made to control TB in the country. Therefore, this study was conducted to investigate the MTBC species and spoligo patterns in the Oromia region (central) of Ethiopia.MethodsA cross-sectional study design was used to recruit 450 smear positive pulmonary TB (PTB) cases from the Oromia region between September 2017 and August 2018. Mycobacteria were isolated from sputum samples on the Lowenstein Jensen (LJ) medium. Molecular identification of the isolates was performed by spoligotyping. The results of spoligotyping were transferred into a query box in the SITVIT2 database and Run TB-Lineage in the TB Insight website for the identification of spoligo international type (SIT) number and linages of the isolates, respectively. Statistical Product and Service Solutions (SPSS) 20 was applied for statistical analysis.ResultsThree hundred and fifteen isolates were grouped under 181 different spoligotype patterns. The most dominantly isolated spoligotype pattern was SIT149 and it consisted of 23 isolates. The majority of the isolates were grouped under Euro-American (EA), East-African-Indian (EAI), and Indo-Oceanic (IO) lineages. These lineages consisted of 79.4, 9.8, and 9.8% of the isolates, respectively. One hundred and sixty-five of the isolates were classified under 31 clustered spoligotypes whereas the remaining 150 were singleton types. Furthermore, 91.1% of the total isolates were classified as orphan types. Clustering of spoligotypes was associated (p < 0.001) with EAI lineage.ConclusionSIT149 and EA lineage were predominantly isolated from the Oromia region substantiating the findings of the similar studies conducted in other regions of Ethiopia. The observation of significant number of singleton and orphan spoligotypes warrants for additional genetic typing of the isolates using method(s) with a better discriminatory power than spoligotyping. |
| first_indexed | 2024-12-13T14:44:40Z |
| format | Article |
| id | doaj.art-9701167ed8704d50b1992746a381f700 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-12-13T14:44:40Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Public Health |
| spelling | doaj.art-9701167ed8704d50b1992746a381f7002022-12-21T23:41:30ZengFrontiers Media S.A.Frontiers in Public Health2296-25652022-03-011010.3389/fpubh.2022.808626808626Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of EthiopiaBedru Hussien0Aboma Zewude1Aboma Zewude2Biniam Wondale3Awraris Hailu4Gobena Ameni5Gobena Ameni6Department of Public Health, Goba Referral Hospital, Madda Walabu University, Goba, EthiopiaMalaria and Neglected Tropical Diseases Research Team, Ethiopian Public Health Institute, Ministry of Health, Addis Ababa, EthiopiaDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Biology, Arba Minch University, Arba Minch, EthiopiaDepartment of Public Health, College of Health Sciences, Debre Birhan University, Debre Birhan, EthiopiaDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain, United Arab EmiratesAklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, EthiopiaBackgroundTuberculosis (TB) is a leading cause of morbidity and mortality in Ethiopia. Investigation of the Mycobacterium tuberculosis complex (MTBC) species circulating in the Ethiopian population would contribute to the efforts made to control TB in the country. Therefore, this study was conducted to investigate the MTBC species and spoligo patterns in the Oromia region (central) of Ethiopia.MethodsA cross-sectional study design was used to recruit 450 smear positive pulmonary TB (PTB) cases from the Oromia region between September 2017 and August 2018. Mycobacteria were isolated from sputum samples on the Lowenstein Jensen (LJ) medium. Molecular identification of the isolates was performed by spoligotyping. The results of spoligotyping were transferred into a query box in the SITVIT2 database and Run TB-Lineage in the TB Insight website for the identification of spoligo international type (SIT) number and linages of the isolates, respectively. Statistical Product and Service Solutions (SPSS) 20 was applied for statistical analysis.ResultsThree hundred and fifteen isolates were grouped under 181 different spoligotype patterns. The most dominantly isolated spoligotype pattern was SIT149 and it consisted of 23 isolates. The majority of the isolates were grouped under Euro-American (EA), East-African-Indian (EAI), and Indo-Oceanic (IO) lineages. These lineages consisted of 79.4, 9.8, and 9.8% of the isolates, respectively. One hundred and sixty-five of the isolates were classified under 31 clustered spoligotypes whereas the remaining 150 were singleton types. Furthermore, 91.1% of the total isolates were classified as orphan types. Clustering of spoligotypes was associated (p < 0.001) with EAI lineage.ConclusionSIT149 and EA lineage were predominantly isolated from the Oromia region substantiating the findings of the similar studies conducted in other regions of Ethiopia. The observation of significant number of singleton and orphan spoligotypes warrants for additional genetic typing of the isolates using method(s) with a better discriminatory power than spoligotyping.https://www.frontiersin.org/articles/10.3389/fpubh.2022.808626/fullMycobacterium tuberculosisspoligotypingclusteringorphansingletonEthiopia |
| spellingShingle | Bedru Hussien Aboma Zewude Aboma Zewude Biniam Wondale Awraris Hailu Gobena Ameni Gobena Ameni Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia Frontiers in Public Health Mycobacterium tuberculosis spoligotyping clustering orphan singleton Ethiopia |
| title | Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia |
| title_full | Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia |
| title_fullStr | Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia |
| title_full_unstemmed | Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia |
| title_short | Spoligotyping of Clinical Isolates of Mycobacterium tuberculosis Complex Species in the Oromia Region of Ethiopia |
| title_sort | spoligotyping of clinical isolates of mycobacterium tuberculosis complex species in the oromia region of ethiopia |
| topic | Mycobacterium tuberculosis spoligotyping clustering orphan singleton Ethiopia |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2022.808626/full |
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