The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota Dysbiosis
<i>Sarcodon aspratus</i> is a popular edible fungus for its tasty flavour and can be used as a dietary supplement for its functional substances. This study was conducted to evaluate the potential health benefits of <i>Sarcodon aspratus</i> polysaccharides (SAFP) on water imme...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/11/1567 |
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| author | Dongjing Zhang Ming Xiang Yun Jiang Fen Wu Huaqun Chen Min Sun Lingzhi Zhang Xianfeng Du Lei Chen |
| author_facet | Dongjing Zhang Ming Xiang Yun Jiang Fen Wu Huaqun Chen Min Sun Lingzhi Zhang Xianfeng Du Lei Chen |
| author_sort | Dongjing Zhang |
| collection | DOAJ |
| description | <i>Sarcodon aspratus</i> is a popular edible fungus for its tasty flavour and can be used as a dietary supplement for its functional substances. This study was conducted to evaluate the potential health benefits of <i>Sarcodon aspratus</i> polysaccharides (SAFP) on water immersion and restraint stress (WIRS)-induced gastric ulcer in rats. The results indicated that SAFP could decrease myeloperoxidase (MPO) activity and plasma corticosterone levels, as well as enhance Prostaglandin E2 (PGE2) and Nitrate/nitrite (NOx) concentration in rats. Furthermore, SAFP significantly attenuated the stress damage, inflammation, pathological changes and gastric mucosal lesion in rats. Moreover, high-throughput pyrosequencing of 16S rRNA suggested that SAFP modulated the dysbiosis of gut microbiota by enhancing the relative abundance of probiotics, decreasing WIRS-triggered bacteria proliferation. In summary, these results provided the evidence that SAFP exerted a beneficial effect on a WIRS-induced gastric ulcer via blocking the TLR4 signaling pathway and activating the Nrf2 signaling pathway. Notably, SAFP could modulate the WIRS-induced dysbiosis of gut microbiota. Thus, SAFP might be explored as a natural gastric mucosal protective agent in the prevention of gastric ulcers and other related diseases in the food and pharmaceutical industries. |
| first_indexed | 2024-03-10T01:20:12Z |
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| id | doaj.art-97024e830c5143cea39e0df39b323ffc |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:20:12Z |
| publishDate | 2022-05-01 |
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| series | Foods |
| spelling | doaj.art-97024e830c5143cea39e0df39b323ffc2023-11-23T14:02:21ZengMDPI AGFoods2304-81582022-05-011111156710.3390/foods11111567The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota DysbiosisDongjing Zhang0Ming Xiang1Yun Jiang2Fen Wu3Huaqun Chen4Min Sun5Lingzhi Zhang6Xianfeng Du7Lei Chen8Anhui Key Laboratory of Eco-Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, ChinaAnhui Key Laboratory of Eco-Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, ChinaAnhui Key Laboratory of Eco-Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, ChinaAnhui Key Laboratory of Eco-Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, ChinaAnhui Key Laboratory of Eco-Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, ChinaAnhui Key Laboratory of Eco-Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, ChinaAnhui Cordyceps Source Biotechnology Co., Ltd., Huainan 232000, ChinaState Key Laboratory of Tea Plant Biology and Utilization, School of Tea and Food Science & Technology, Anhui Agricultural University, Hefei 230036, ChinaAnhui Key Laboratory of Eco-Engineering and Biotechnology, School of Life Sciences, Anhui University, Hefei 230601, China<i>Sarcodon aspratus</i> is a popular edible fungus for its tasty flavour and can be used as a dietary supplement for its functional substances. This study was conducted to evaluate the potential health benefits of <i>Sarcodon aspratus</i> polysaccharides (SAFP) on water immersion and restraint stress (WIRS)-induced gastric ulcer in rats. The results indicated that SAFP could decrease myeloperoxidase (MPO) activity and plasma corticosterone levels, as well as enhance Prostaglandin E2 (PGE2) and Nitrate/nitrite (NOx) concentration in rats. Furthermore, SAFP significantly attenuated the stress damage, inflammation, pathological changes and gastric mucosal lesion in rats. Moreover, high-throughput pyrosequencing of 16S rRNA suggested that SAFP modulated the dysbiosis of gut microbiota by enhancing the relative abundance of probiotics, decreasing WIRS-triggered bacteria proliferation. In summary, these results provided the evidence that SAFP exerted a beneficial effect on a WIRS-induced gastric ulcer via blocking the TLR4 signaling pathway and activating the Nrf2 signaling pathway. Notably, SAFP could modulate the WIRS-induced dysbiosis of gut microbiota. Thus, SAFP might be explored as a natural gastric mucosal protective agent in the prevention of gastric ulcers and other related diseases in the food and pharmaceutical industries.https://www.mdpi.com/2304-8158/11/11/1567<i>Sarcodon aspratus</i> polysaccharidesWIRS-induced gastric ulcergut microbiotasignaling pathway |
| spellingShingle | Dongjing Zhang Ming Xiang Yun Jiang Fen Wu Huaqun Chen Min Sun Lingzhi Zhang Xianfeng Du Lei Chen The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota Dysbiosis Foods <i>Sarcodon aspratus</i> polysaccharides WIRS-induced gastric ulcer gut microbiota signaling pathway |
| title | The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota Dysbiosis |
| title_full | The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota Dysbiosis |
| title_fullStr | The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota Dysbiosis |
| title_full_unstemmed | The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota Dysbiosis |
| title_short | The Protective Effect of Polysaccharide SAFP from <i>Sarcodon aspratus</i> on Water Immersion and Restraint Stress-Induced Gastric Ulcer and Modulatory Effects on Gut Microbiota Dysbiosis |
| title_sort | protective effect of polysaccharide safp from i sarcodon aspratus i on water immersion and restraint stress induced gastric ulcer and modulatory effects on gut microbiota dysbiosis |
| topic | <i>Sarcodon aspratus</i> polysaccharides WIRS-induced gastric ulcer gut microbiota signaling pathway |
| url | https://www.mdpi.com/2304-8158/11/11/1567 |
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