Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran
Objective(s): It has been reported that the mutation of the pre-core (PC) and basal-core promoter (BCP) may play an important role in the development of HBV-related hepatocellular carcinoma (HCC). In this study the PC and BCP mutations were investigated in chronic HBV patients. Materials and Methods...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2014-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_2787_abbab5d33dc68d927b2a932f984726c3.html |
| _version_ | 1811311540615249920 |
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| author | Abdolvahab Moradi Sareh Zhand Amir Ghaemi Naeme Javid Masoud Bazouri Alijan Tabarraei |
| author_facet | Abdolvahab Moradi Sareh Zhand Amir Ghaemi Naeme Javid Masoud Bazouri Alijan Tabarraei |
| author_sort | Abdolvahab Moradi |
| collection | DOAJ |
| description | Objective(s): It has been reported that the mutation of the pre-core (PC) and basal-core promoter (BCP) may play an important role in the development of HBV-related hepatocellular carcinoma (HCC). In this study the PC and BCP mutations were investigated in chronic HBV patients. Materials and Methods:In this study, 120 chronic HBV patients from Golestan, Northeast of Iran who were not vaccinated against HBV, were recruited from the year 2008 to 2012. HBV-DNA extraction from plasma and PCR were performed and positive PCR products were subjected to automated sequencing. Results:One hundred out of 120 (83.3%) patients were HBeAg negative. Comparison of our nucleotide sequences with reference sequence showed high rate mutation in BCP and PC region (96.66%). Frame shift mutation was found in 78 (65%) of patients in BCP region, among them 8 (6.6%) patients showed mutation in PC region. Conclusion: Our results demonstrated high rate of mutations in BCP and PC regions among HBV chronic patients in Northeast of Iran. |
| first_indexed | 2024-04-13T10:20:51Z |
| format | Article |
| id | doaj.art-970352195faf4d5fb15f6b8af596d319 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-13T10:20:51Z |
| publishDate | 2014-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-970352195faf4d5fb15f6b8af596d3192022-12-22T02:50:31ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742014-05-011753703772787Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, IranAbdolvahab Moradi0Sareh Zhand1Amir Ghaemi2Naeme Javid3Masoud Bazouri4Alijan Tabarraei5Infectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranInfectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranInfectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranInfectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranInfectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranInfectious Diseases Research Center, Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IranObjective(s): It has been reported that the mutation of the pre-core (PC) and basal-core promoter (BCP) may play an important role in the development of HBV-related hepatocellular carcinoma (HCC). In this study the PC and BCP mutations were investigated in chronic HBV patients. Materials and Methods:In this study, 120 chronic HBV patients from Golestan, Northeast of Iran who were not vaccinated against HBV, were recruited from the year 2008 to 2012. HBV-DNA extraction from plasma and PCR were performed and positive PCR products were subjected to automated sequencing. Results:One hundred out of 120 (83.3%) patients were HBeAg negative. Comparison of our nucleotide sequences with reference sequence showed high rate mutation in BCP and PC region (96.66%). Frame shift mutation was found in 78 (65%) of patients in BCP region, among them 8 (6.6%) patients showed mutation in PC region. Conclusion: Our results demonstrated high rate of mutations in BCP and PC regions among HBV chronic patients in Northeast of Iran.http://ijbms.mums.ac.ir/pdf_2787_abbab5d33dc68d927b2a932f984726c3.htmlBCP mutationHepatitis BIranPC mutation |
| spellingShingle | Abdolvahab Moradi Sareh Zhand Amir Ghaemi Naeme Javid Masoud Bazouri Alijan Tabarraei Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran Iranian Journal of Basic Medical Sciences BCP mutation Hepatitis B Iran PC mutation |
| title | Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran |
| title_full | Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran |
| title_fullStr | Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran |
| title_full_unstemmed | Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran |
| title_short | Mutations in pre-core and basal-core promoter regions of hepatitis B virus in chronic HBV patients from Golestan, Iran |
| title_sort | mutations in pre core and basal core promoter regions of hepatitis b virus in chronic hbv patients from golestan iran |
| topic | BCP mutation Hepatitis B Iran PC mutation |
| url | http://ijbms.mums.ac.ir/pdf_2787_abbab5d33dc68d927b2a932f984726c3.html |
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