Mice lacking galectin-3 (Lgals3) function have decreased home cage movement
Abstract Background Galectins are a large family of proteins evolved to recognize specific carbohydrate moieties. Given the importance of pattern recognition processes for multiple biological tasks, including CNS development and immune recognition, we examined the home cage behavioral phenotype of m...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-05-01
|
| Series: | BMC Neuroscience |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12868-018-0428-x |
| _version_ | 1818177738976002048 |
|---|---|
| author | Tammy R. Chaudoin Stephen J. Bonasera |
| author_facet | Tammy R. Chaudoin Stephen J. Bonasera |
| author_sort | Tammy R. Chaudoin |
| collection | DOAJ |
| description | Abstract Background Galectins are a large family of proteins evolved to recognize specific carbohydrate moieties. Given the importance of pattern recognition processes for multiple biological tasks, including CNS development and immune recognition, we examined the home cage behavioral phenotype of mice lacking galectin-3 (Lgals3) function. Using a sophisticated monitoring apparatus capable of examining feeding, drinking, and movement at millisecond temporal and 0.5 cm spatial resolutions, we observed daily behavioral patterns from 10 wildtype male C57BL/6J and 10 Lgals3 constitutive knockout (Lgals3 −/−; both cohorts aged 2–3 months) mice over 17 consecutive days. We performed a second behavioral assessment of this cohort at age 6–7 months. Results At both ages, Lgals3 −/− mice demonstrated less movement compared to wildtype controls. Both forward locomotion and movement-in-place behaviors were decreased in Lgals3 −/− mice, due to decreased bout numbers, initiation rates, and durations. We additionally noted perturbation of behavioral circadian rhythms in Lgals3 −/− mice, with mice at both ages demonstrating greater variability in day-to-day performance of feeding, drinking, and movement (as assessed by Lomb-Scargle analysis) compared to wildtype. Conclusion Carbohydrate recognition tasks performed by Lgals3 may be required for appropriate development of CNS structures involved in the generation and control of locomotor behavior. |
| first_indexed | 2024-12-11T20:36:52Z |
| format | Article |
| id | doaj.art-970412627cef47b2ae3ad5354a592afc |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-11T20:36:52Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-970412627cef47b2ae3ad5354a592afc2022-12-22T00:51:38ZengBMCBMC Neuroscience1471-22022018-05-0119111010.1186/s12868-018-0428-xMice lacking galectin-3 (Lgals3) function have decreased home cage movementTammy R. Chaudoin0Stephen J. Bonasera1Division of Geriatrics, Department of Internal Medicine, University of Nebraska Medical CenterDivision of Geriatrics, Department of Internal Medicine, University of Nebraska Medical CenterAbstract Background Galectins are a large family of proteins evolved to recognize specific carbohydrate moieties. Given the importance of pattern recognition processes for multiple biological tasks, including CNS development and immune recognition, we examined the home cage behavioral phenotype of mice lacking galectin-3 (Lgals3) function. Using a sophisticated monitoring apparatus capable of examining feeding, drinking, and movement at millisecond temporal and 0.5 cm spatial resolutions, we observed daily behavioral patterns from 10 wildtype male C57BL/6J and 10 Lgals3 constitutive knockout (Lgals3 −/−; both cohorts aged 2–3 months) mice over 17 consecutive days. We performed a second behavioral assessment of this cohort at age 6–7 months. Results At both ages, Lgals3 −/− mice demonstrated less movement compared to wildtype controls. Both forward locomotion and movement-in-place behaviors were decreased in Lgals3 −/− mice, due to decreased bout numbers, initiation rates, and durations. We additionally noted perturbation of behavioral circadian rhythms in Lgals3 −/− mice, with mice at both ages demonstrating greater variability in day-to-day performance of feeding, drinking, and movement (as assessed by Lomb-Scargle analysis) compared to wildtype. Conclusion Carbohydrate recognition tasks performed by Lgals3 may be required for appropriate development of CNS structures involved in the generation and control of locomotor behavior.http://link.springer.com/article/10.1186/s12868-018-0428-xCircadian rhythmGalectin-3Ingestive behaviorLgals3Locomotor behavior |
| spellingShingle | Tammy R. Chaudoin Stephen J. Bonasera Mice lacking galectin-3 (Lgals3) function have decreased home cage movement BMC Neuroscience Circadian rhythm Galectin-3 Ingestive behavior Lgals3 Locomotor behavior |
| title | Mice lacking galectin-3 (Lgals3) function have decreased home cage movement |
| title_full | Mice lacking galectin-3 (Lgals3) function have decreased home cage movement |
| title_fullStr | Mice lacking galectin-3 (Lgals3) function have decreased home cage movement |
| title_full_unstemmed | Mice lacking galectin-3 (Lgals3) function have decreased home cage movement |
| title_short | Mice lacking galectin-3 (Lgals3) function have decreased home cage movement |
| title_sort | mice lacking galectin 3 lgals3 function have decreased home cage movement |
| topic | Circadian rhythm Galectin-3 Ingestive behavior Lgals3 Locomotor behavior |
| url | http://link.springer.com/article/10.1186/s12868-018-0428-x |
| work_keys_str_mv | AT tammyrchaudoin micelackinggalectin3lgals3functionhavedecreasedhomecagemovement AT stephenjbonasera micelackinggalectin3lgals3functionhavedecreasedhomecagemovement |