Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model
Tobacco smoking is one of the most serious health problems. Potentially lethal effects of nicotine for adults can occur with as little as 30 to 60 mg, although severe symptoms can arise with lower doses. Furthermore, the route of administration also influences the toxicity. Cytisine is one of the mo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12094 |
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| author | Wojciech Świątkowski Barbara Budzyńska Monika Maciąg Agnieszka Świątkowska Przemko Tylżanowski Mansur Rahnama-Hezavah Piotr Stachurski Renata Chałas |
| author_facet | Wojciech Świątkowski Barbara Budzyńska Monika Maciąg Agnieszka Świątkowska Przemko Tylżanowski Mansur Rahnama-Hezavah Piotr Stachurski Renata Chałas |
| author_sort | Wojciech Świątkowski |
| collection | DOAJ |
| description | Tobacco smoking is one of the most serious health problems. Potentially lethal effects of nicotine for adults can occur with as little as 30 to 60 mg, although severe symptoms can arise with lower doses. Furthermore, the route of administration also influences the toxicity. Cytisine is one of the most popular medications in nicotinism treatment. Like nicotine, cytisine is a plant alkaloid, signaling through nicotinic acetylcholine receptors. Our study evaluated the effects of cytisine in nicotine-induced embryotoxic effects using zebrafish larvae. We examined the teratogenicity of nicotine and cytisine alone or in combination. Nicotine increased mortality and delayed hatching of zebrafish larvae in a dose-dependent manner. Cytisine did not affect mortality in a wide range of concentrations, and hatching delay was observed only at the highest concentrations, above 2 mM. Administering compounds together partially reduced the adverse teratogenic effect induced by nicotine alone. The protective effect of cytisine against the nicotine effect, observed in zebrafish, will contribute to future studies or treatments related to nicotine addiction or prenatal nicotine exposure in humans. |
| first_indexed | 2024-03-11T00:26:38Z |
| format | Article |
| id | doaj.art-970421040b8c4891aba175b2db2d0000 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:26:38Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-970421040b8c4891aba175b2db2d00002023-11-18T23:00:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124151209410.3390/ijms241512094Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish ModelWojciech Świątkowski0Barbara Budzyńska1Monika Maciąg2Agnieszka Świątkowska3Przemko Tylżanowski4Mansur Rahnama-Hezavah5Piotr Stachurski6Renata Chałas7Department of Oral Surgery, Medical University of Lublin, 20-059 Lublin, PolandIndependent Laboratory of Behavioral Studies, Medical University of Lublin, 20-059 Lublin, PolandIndependent Laboratory of Behavioral Studies, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Jaw Orthopaedics, Medical University of Lublin, 20-059 Lublin, PolandLaboratory of Molecular Genetics, Department of Biomedical Sciences, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Oral Surgery, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Developmental Dentistry, Medical University of Lublin, 20-059 Lublin, PolandDepartment of Oral Medicine, Medical University of Lublin, 20-059 Lublin, PolandTobacco smoking is one of the most serious health problems. Potentially lethal effects of nicotine for adults can occur with as little as 30 to 60 mg, although severe symptoms can arise with lower doses. Furthermore, the route of administration also influences the toxicity. Cytisine is one of the most popular medications in nicotinism treatment. Like nicotine, cytisine is a plant alkaloid, signaling through nicotinic acetylcholine receptors. Our study evaluated the effects of cytisine in nicotine-induced embryotoxic effects using zebrafish larvae. We examined the teratogenicity of nicotine and cytisine alone or in combination. Nicotine increased mortality and delayed hatching of zebrafish larvae in a dose-dependent manner. Cytisine did not affect mortality in a wide range of concentrations, and hatching delay was observed only at the highest concentrations, above 2 mM. Administering compounds together partially reduced the adverse teratogenic effect induced by nicotine alone. The protective effect of cytisine against the nicotine effect, observed in zebrafish, will contribute to future studies or treatments related to nicotine addiction or prenatal nicotine exposure in humans.https://www.mdpi.com/1422-0067/24/15/12094tobaccopregnancydevelopementzebrafishtoxicity attenuation |
| spellingShingle | Wojciech Świątkowski Barbara Budzyńska Monika Maciąg Agnieszka Świątkowska Przemko Tylżanowski Mansur Rahnama-Hezavah Piotr Stachurski Renata Chałas Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model International Journal of Molecular Sciences tobacco pregnancy developement zebrafish toxicity attenuation |
| title | Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model |
| title_full | Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model |
| title_fullStr | Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model |
| title_full_unstemmed | Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model |
| title_short | Nicotine and Cytisine Embryotoxicity in the Experimental Zebrafish Model |
| title_sort | nicotine and cytisine embryotoxicity in the experimental zebrafish model |
| topic | tobacco pregnancy developement zebrafish toxicity attenuation |
| url | https://www.mdpi.com/1422-0067/24/15/12094 |
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