Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in Ahvaz
Abstract Background: Pseudomonas aeruginosa is one of the major etiologic agents of nosocomial infection among burn patients that has high resistance to antibiotics. Integrons can extend antibiotic resistance genes among bacteria. The aim of this study was to investigate the antibiotic resistance p...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Arak Medical University
2015-06-01
|
| Series: | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| Subjects: | |
| Online Access: | http://amuj.arakmu.ac.ir/article-1-3725-en.pdf |
| _version_ | 1818392469417492480 |
|---|---|
| author | Seyyed Sajjad Khorramrooz Farzaneh Gharibpour Najmeh Parhizgari Mahboobeh Yazdanpanah Reza Mohammadi Nasim Rahbari |
| author_facet | Seyyed Sajjad Khorramrooz Farzaneh Gharibpour Najmeh Parhizgari Mahboobeh Yazdanpanah Reza Mohammadi Nasim Rahbari |
| author_sort | Seyyed Sajjad Khorramrooz |
| collection | DOAJ |
| description | Abstract
Background: Pseudomonas aeruginosa is one of the major etiologic agents of nosocomial infection among burn patients that has high resistance to antibiotics. Integrons can extend antibiotic resistance genes among bacteria. The aim of this study was to investigate the antibiotic resistance pattern and the prevalence of integron among P. aeruginosa isolates.
Materials and Methods: In this cross-sectional study, a total of 73 P. aeruginosa isolated from burn wound infections among hospitalized patients in Ahvaz Taleghani hospital. Antibiotic resistance pattern of these bacteria was investigated to 9 antibiotics by Disk Agar Diffusion method. Polymerase chain reaction (PCR) method was used to investigate the prevalence of class 1, 2 and 3 integrons. The data were analyzed by Chi-square test. A P-value of <0.05 was considered as a statistical significance level.
Results: The most antibiotic resistance level was seen against ofloxacin (94.5%), aztreonam (94.5%), and ceftazidime (93.6%). Fifteen isolates of P. aeruginosa were resistance to all of the antibiotics. The study of molecular results showed that class 1 integron was detected in 35.6% of isolates, while none of them harbored class 2 and 3 integron.
Conclusion: The rates of antibiotic resistance in pseudomonas aeruginosa to antibiotics such as ceftazidime, oflaxacin, aztreonam, cefepime, and ceftriaxone is very high. Although, class 1 integron were detected in 35.6% of isolates, there was no statistically significant differences between the presence of integron and resistance to a specific antibiotic, that it shows the role of the other antibiotic resistance mechanisms among pseudomonas aeruginosa. |
| first_indexed | 2024-12-14T05:29:55Z |
| format | Article |
| id | doaj.art-9704fb0bf4ed48418a311f4d60159fce |
| institution | Directory Open Access Journal |
| issn | 1735-5338 2008-644X |
| language | fas |
| last_indexed | 2024-12-14T05:29:55Z |
| publishDate | 2015-06-01 |
| publisher | Arak Medical University |
| record_format | Article |
| series | Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk |
| spelling | doaj.art-9704fb0bf4ed48418a311f4d60159fce2022-12-21T23:15:22ZfasArak Medical UniversityMajallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk1735-53382008-644X2015-06-01183918Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in AhvazSeyyed Sajjad Khorramrooz0Farzaneh Gharibpour1Najmeh Parhizgari2Mahboobeh Yazdanpanah3Reza Mohammadi4Nasim Rahbari5Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.Department of Pathobiology, Shiraz University, Shiraz, IranDepartment of Virology, Tehran University of Medical Sciences, Tehran, Iran.Student Research Committee, Yasuj University of Medical Sciences, Yasuj, IranMedicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.Department of Basic Sciences, Islamic Azad University, Sciences and Research Complementaty Education Pardis of Kohgiluyeh-va-Boyerahmad, Yasouj, Iran.Abstract Background: Pseudomonas aeruginosa is one of the major etiologic agents of nosocomial infection among burn patients that has high resistance to antibiotics. Integrons can extend antibiotic resistance genes among bacteria. The aim of this study was to investigate the antibiotic resistance pattern and the prevalence of integron among P. aeruginosa isolates. Materials and Methods: In this cross-sectional study, a total of 73 P. aeruginosa isolated from burn wound infections among hospitalized patients in Ahvaz Taleghani hospital. Antibiotic resistance pattern of these bacteria was investigated to 9 antibiotics by Disk Agar Diffusion method. Polymerase chain reaction (PCR) method was used to investigate the prevalence of class 1, 2 and 3 integrons. The data were analyzed by Chi-square test. A P-value of <0.05 was considered as a statistical significance level. Results: The most antibiotic resistance level was seen against ofloxacin (94.5%), aztreonam (94.5%), and ceftazidime (93.6%). Fifteen isolates of P. aeruginosa were resistance to all of the antibiotics. The study of molecular results showed that class 1 integron was detected in 35.6% of isolates, while none of them harbored class 2 and 3 integron. Conclusion: The rates of antibiotic resistance in pseudomonas aeruginosa to antibiotics such as ceftazidime, oflaxacin, aztreonam, cefepime, and ceftriaxone is very high. Although, class 1 integron were detected in 35.6% of isolates, there was no statistically significant differences between the presence of integron and resistance to a specific antibiotic, that it shows the role of the other antibiotic resistance mechanisms among pseudomonas aeruginosa.http://amuj.arakmu.ac.ir/article-1-3725-en.pdfAntibiotic resistanceBurn unitIntegronPseudomonas aeruginosa |
| spellingShingle | Seyyed Sajjad Khorramrooz Farzaneh Gharibpour Najmeh Parhizgari Mahboobeh Yazdanpanah Reza Mohammadi Nasim Rahbari Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in Ahvaz Majallah-i dānishgāh-i ̒ulūm-i pizishkī-i Arāk Antibiotic resistance Burn unit Integron Pseudomonas aeruginosa |
| title | Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in Ahvaz |
| title_full | Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in Ahvaz |
| title_fullStr | Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in Ahvaz |
| title_full_unstemmed | Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in Ahvaz |
| title_short | Prevalence of Class 1 Integron and Antibiotic Resistance among Pseudomonas Aeruginosa Isolated from Patients Admitted to the Burn Unit at Taleghani Hospital in Ahvaz |
| title_sort | prevalence of class 1 integron and antibiotic resistance among pseudomonas aeruginosa isolated from patients admitted to the burn unit at taleghani hospital in ahvaz |
| topic | Antibiotic resistance Burn unit Integron Pseudomonas aeruginosa |
| url | http://amuj.arakmu.ac.ir/article-1-3725-en.pdf |
| work_keys_str_mv | AT seyyedsajjadkhorramrooz prevalenceofclass1integronandantibioticresistanceamongpseudomonasaeruginosaisolatedfrompatientsadmittedtotheburnunitattaleghanihospitalinahvaz AT farzanehgharibpour prevalenceofclass1integronandantibioticresistanceamongpseudomonasaeruginosaisolatedfrompatientsadmittedtotheburnunitattaleghanihospitalinahvaz AT najmehparhizgari prevalenceofclass1integronandantibioticresistanceamongpseudomonasaeruginosaisolatedfrompatientsadmittedtotheburnunitattaleghanihospitalinahvaz AT mahboobehyazdanpanah prevalenceofclass1integronandantibioticresistanceamongpseudomonasaeruginosaisolatedfrompatientsadmittedtotheburnunitattaleghanihospitalinahvaz AT rezamohammadi prevalenceofclass1integronandantibioticresistanceamongpseudomonasaeruginosaisolatedfrompatientsadmittedtotheburnunitattaleghanihospitalinahvaz AT nasimrahbari prevalenceofclass1integronandantibioticresistanceamongpseudomonasaeruginosaisolatedfrompatientsadmittedtotheburnunitattaleghanihospitalinahvaz |