Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i>
The dioctadecyldimethylammonium bromide (DODAB) is a double-chained cationic lipid with potent bactericide and fungistatic activities; however, its toxicity on protozoan parasites is still unknown. Here, we show the antileishmanial activity of DODAB nano-sized cationic bilayer fragments on stationar...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/23/5741 |
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| author | Thalita C. S. Ferreira Ismael P. Sauter Lina Borda-Samper Enyd Bentivoglio Jarina P. DaMata Noemi N. Taniwaki Patrício R. Orrego Jorge E. Araya Nilton Lincopan Mauro Cortez |
| author_facet | Thalita C. S. Ferreira Ismael P. Sauter Lina Borda-Samper Enyd Bentivoglio Jarina P. DaMata Noemi N. Taniwaki Patrício R. Orrego Jorge E. Araya Nilton Lincopan Mauro Cortez |
| author_sort | Thalita C. S. Ferreira |
| collection | DOAJ |
| description | The dioctadecyldimethylammonium bromide (DODAB) is a double-chained cationic lipid with potent bactericide and fungistatic activities; however, its toxicity on protozoan parasites is still unknown. Here, we show the antileishmanial activity of DODAB nano-sized cationic bilayer fragments on stationary-phase promastigotes and amastigotes of <i>Leishmania amazonensis</i>, the causative agent of cutaneous leishmaniasis. Upon treatment with DODAB, we analyzed the parasite surface zeta-potential, parasite viability, cellular structural modifications, and intracellular proliferation. The DODAB cytotoxic effect was dose-dependent, with a median effective concentration (EC<sub>50</sub>) of 25 µM for both life-cycle stages, comparable to the reported data for bacteria and fungi. The treatment with DODAB changed the membrane zeta-potential from negative to positive, compromised the parasite’s morphology, affected the cell size regulation, caused a loss of intracellular organelles, and probably dysregulated the plasma membrane permeability without membrane disruption. Moreover, the parasites that survived after treatment induced small parasitophorous vacuoles and failed to proliferate inside macrophages. In conclusion, DODAB displayed antileishmanial activity, and it remains to be elucidated how DODAB acts on the protozoan membrane. Understanding this mechanism can provide insights into the development of new parasite-control strategies. |
| first_indexed | 2024-03-10T14:18:37Z |
| format | Article |
| id | doaj.art-97064c9eeecb48a4aa19d12ef1807f07 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:18:37Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-97064c9eeecb48a4aa19d12ef1807f072023-11-20T23:35:49ZengMDPI AGMolecules1420-30492020-12-012523574110.3390/molecules25235741Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i>Thalita C. S. Ferreira0Ismael P. Sauter1Lina Borda-Samper2Enyd Bentivoglio3Jarina P. DaMata4Noemi N. Taniwaki5Patrício R. Orrego6Jorge E. Araya7Nilton Lincopan8Mauro Cortez9Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilInstituto Adolfo Lutz, São Paulo, SP 02146-000, BrazilFacultad de Ciencias de la Salud, Departamento Biomédico, Universidad de Antofagasta, Antofagasta, CP 1270300, ChileFacultad de Ciências de la Salud, Departamento de Tecnología Médica, Universidad de Antofagasta, Antofagasta, CP 1270300, ChileDepartamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilDepartamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP 05508-000, BrazilThe dioctadecyldimethylammonium bromide (DODAB) is a double-chained cationic lipid with potent bactericide and fungistatic activities; however, its toxicity on protozoan parasites is still unknown. Here, we show the antileishmanial activity of DODAB nano-sized cationic bilayer fragments on stationary-phase promastigotes and amastigotes of <i>Leishmania amazonensis</i>, the causative agent of cutaneous leishmaniasis. Upon treatment with DODAB, we analyzed the parasite surface zeta-potential, parasite viability, cellular structural modifications, and intracellular proliferation. The DODAB cytotoxic effect was dose-dependent, with a median effective concentration (EC<sub>50</sub>) of 25 µM for both life-cycle stages, comparable to the reported data for bacteria and fungi. The treatment with DODAB changed the membrane zeta-potential from negative to positive, compromised the parasite’s morphology, affected the cell size regulation, caused a loss of intracellular organelles, and probably dysregulated the plasma membrane permeability without membrane disruption. Moreover, the parasites that survived after treatment induced small parasitophorous vacuoles and failed to proliferate inside macrophages. In conclusion, DODAB displayed antileishmanial activity, and it remains to be elucidated how DODAB acts on the protozoan membrane. Understanding this mechanism can provide insights into the development of new parasite-control strategies.https://www.mdpi.com/1420-3049/25/23/5741antileishmanial activitycationic bilayer fragmentsdioctadecyldimethylammonium bromide (DODAB)<i>Leishmania amazonensis</i> |
| spellingShingle | Thalita C. S. Ferreira Ismael P. Sauter Lina Borda-Samper Enyd Bentivoglio Jarina P. DaMata Noemi N. Taniwaki Patrício R. Orrego Jorge E. Araya Nilton Lincopan Mauro Cortez Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i> Molecules antileishmanial activity cationic bilayer fragments dioctadecyldimethylammonium bromide (DODAB) <i>Leishmania amazonensis</i> |
| title | Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i> |
| title_full | Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i> |
| title_fullStr | Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i> |
| title_full_unstemmed | Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i> |
| title_short | Effect of DODAB Nano-Sized Cationic Bilayer Fragments against <i>Leishmania amazonensis</i> |
| title_sort | effect of dodab nano sized cationic bilayer fragments against i leishmania amazonensis i |
| topic | antileishmanial activity cationic bilayer fragments dioctadecyldimethylammonium bromide (DODAB) <i>Leishmania amazonensis</i> |
| url | https://www.mdpi.com/1420-3049/25/23/5741 |
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