Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel
Abstract Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engine...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-05434-6 |
| _version_ | 1818430388275511296 |
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| author | Ilaria Sanzari Mauro Callisti Antonio De Grazia Daniel J. Evans Tomas Polcar Themistoklis Prodromakis |
| author_facet | Ilaria Sanzari Mauro Callisti Antonio De Grazia Daniel J. Evans Tomas Polcar Themistoklis Prodromakis |
| author_sort | Ilaria Sanzari |
| collection | DOAJ |
| description | Abstract Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm. Polydimethylsiloxane (PDMS) and Polyacrylamide (PAm) hydrogel have been chosen as soft polymers to be moulded. Thanks to their chemical and physical properties PDMS and PAm hydrogel mimic the extracellular matrix (ECM). PDMS was spin coated on micropatterned Parylene C obtaining composite substrates with 460 nm and 1.15 µm high grooves. The Young’s modulus of the composite Parylene C/PDMS was evaluated and it was found to be almost half when compared to PDMS. PAm hydrogel was also printed using collagen coated micro-grooved Parylene C. Optical micrographs and fluorescence analysis show the successful topographic and protein pattern transfer on the hydrogel. |
| first_indexed | 2024-12-14T15:32:37Z |
| format | Article |
| id | doaj.art-9708a87e10894147856f35193dd664e0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-14T15:32:37Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9708a87e10894147856f35193dd664e02022-12-21T22:55:50ZengNature PortfolioScientific Reports2045-23222017-07-017111110.1038/s41598-017-05434-6Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogelIlaria Sanzari0Mauro Callisti1Antonio De Grazia2Daniel J. Evans3Tomas Polcar4Themistoklis Prodromakis5Nanoelectronics & Nanotechnology Research Group, Department of Electronics and Computer Science, Faculty of Physical Science and EngineeringEngineering ScienceDepartment of Electronics and Computer ScienceNanoelectronics & Nanotechnology Research Group, Department of Electronics and Computer Science, Faculty of Physical Science and EngineeringEngineering ScienceNanoelectronics & Nanotechnology Research Group, Department of Electronics and Computer Science, Faculty of Physical Science and EngineeringAbstract Parylene C is a well-known polymer and it has been mainly employed as a protective layer for implantable electronics. In this paper, we propose a new approach to use Parylene C as a versatile template for patterning soft materials potentially applicable as scaffolds in cardiac tissue engineering (TE). Parylene C substrates were anisotropically patterned through standard lithographic process with hydrophilic channels separating raised hydrophobic strips. Ridges and grooves of the template are 10 µm width and depth ranging from 1 to 17 µm. Polydimethylsiloxane (PDMS) and Polyacrylamide (PAm) hydrogel have been chosen as soft polymers to be moulded. Thanks to their chemical and physical properties PDMS and PAm hydrogel mimic the extracellular matrix (ECM). PDMS was spin coated on micropatterned Parylene C obtaining composite substrates with 460 nm and 1.15 µm high grooves. The Young’s modulus of the composite Parylene C/PDMS was evaluated and it was found to be almost half when compared to PDMS. PAm hydrogel was also printed using collagen coated micro-grooved Parylene C. Optical micrographs and fluorescence analysis show the successful topographic and protein pattern transfer on the hydrogel.https://doi.org/10.1038/s41598-017-05434-6 |
| spellingShingle | Ilaria Sanzari Mauro Callisti Antonio De Grazia Daniel J. Evans Tomas Polcar Themistoklis Prodromakis Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel Scientific Reports |
| title | Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel |
| title_full | Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel |
| title_fullStr | Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel |
| title_full_unstemmed | Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel |
| title_short | Parylene C topographic micropattern as a template for patterning PDMS and Polyacrylamide hydrogel |
| title_sort | parylene c topographic micropattern as a template for patterning pdms and polyacrylamide hydrogel |
| url | https://doi.org/10.1038/s41598-017-05434-6 |
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