NMR Assignment of Methyl Groups in Immobilized Proteins Using Multiple-Bond 13C Homonuclear Transfers, Proton Detection, and Very Fast MAS

NMR Assignment of Methyl Groups in Immobilized Proteins Using Multiple-Bond 13C Homonuclear Transfers, Proton Detection, and Very Fast MAS

In nuclear magnetic resonance spectroscopy of proteins, methyl protons play a particular role as extremely sensitive reporters on dynamics, allosteric effects, and protein–protein interactions, accessible even in high-molecular-weight systems approaching 1 MDa. The notorious issue of their chemical...

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Bibliographic Details
Main Authors: Piotr Paluch, Rafal Augustyniak, Mai-Liis Org, Kalju Vanatalu, Ats Kaldma, Ago Samoson, Jan Stanek
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-03-01
Series:Frontiers in Molecular Biosciences
Subjects:
NMR resonance assignment
methyl groups
solid-state NMR
fast MAS
proton detection
TOCSY
Online Access:https://www.frontiersin.org/articles/10.3389/fmolb.2022.828785/full

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