Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-Desaturase
In this study, delta-12 desaturase was overexpressed in <i>Yarrowia lipolytica</i> using the single-copy integrative vector pINA1312 and multicopy integrative vector pINA1292, resulting in the engineered yeast strains 1312-12 and 1292-12, respectively. The content of intracellular linole...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/10/2/126 |
| _version_ | 1797297753399754752 |
|---|---|
| author | Yufei Chang Zhen Wang Hequn Li Wenrui Dang Yuanda Song Xinxin Kang Huaiyuan Zhang |
| author_facet | Yufei Chang Zhen Wang Hequn Li Wenrui Dang Yuanda Song Xinxin Kang Huaiyuan Zhang |
| author_sort | Yufei Chang |
| collection | DOAJ |
| description | In this study, delta-12 desaturase was overexpressed in <i>Yarrowia lipolytica</i> using the single-copy integrative vector pINA1312 and multicopy integrative vector pINA1292, resulting in the engineered yeast strains 1312-12 and 1292-12, respectively. The content of intracellular linoleic acid (LA) in the 1292-12 strain was much higher than in the 1312-12 strain and the control group. One interesting finding was that the 1292-12 strain showed obvious changes in surface morphology. The 1292-12 colonies were much smaller and smoother, whereas their single cells became much larger compared to the control strain. In addition, the dry cell weight (DCW) of the 1292-12 strain was obviously increased from 8.5 to 12.7 g/L, but the viable cell number sharply decreased from 10<sup>7</sup> to 10<sup>5</sup>/mL. These results indicated that increased LA content in <i>Yarrowia lipolytica</i> could induce morphological changes or even oxidative stress-dependent cell death. The reactive oxygen species (ROS) and malondialdehyde (MDA) were accumulated in the 1292-12 strain, while the antioxidant activities of intracellular catalase (CAT) and superoxide dismutase (SOD) were significantly decreased by 27.6 and 32.0%, respectively. Furthermore, it was also revealed that these issues could be ameliorated by the exogenous supplementation of vitamin C, fish and colza oil. |
| first_indexed | 2024-03-07T22:25:54Z |
| format | Article |
| id | doaj.art-970a5e9fea7b4ccaba9bbe1ac8baef9a |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-07T22:25:54Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-970a5e9fea7b4ccaba9bbe1ac8baef9a2024-02-23T15:23:30ZengMDPI AGJournal of Fungi2309-608X2024-02-0110212610.3390/jof10020126Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-DesaturaseYufei Chang0Zhen Wang1Hequn Li2Wenrui Dang3Yuanda Song4Xinxin Kang5Huaiyuan Zhang6Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaSchool of Public Health, Qilu Medical University, Zibo 255300, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaColin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255049, ChinaIn this study, delta-12 desaturase was overexpressed in <i>Yarrowia lipolytica</i> using the single-copy integrative vector pINA1312 and multicopy integrative vector pINA1292, resulting in the engineered yeast strains 1312-12 and 1292-12, respectively. The content of intracellular linoleic acid (LA) in the 1292-12 strain was much higher than in the 1312-12 strain and the control group. One interesting finding was that the 1292-12 strain showed obvious changes in surface morphology. The 1292-12 colonies were much smaller and smoother, whereas their single cells became much larger compared to the control strain. In addition, the dry cell weight (DCW) of the 1292-12 strain was obviously increased from 8.5 to 12.7 g/L, but the viable cell number sharply decreased from 10<sup>7</sup> to 10<sup>5</sup>/mL. These results indicated that increased LA content in <i>Yarrowia lipolytica</i> could induce morphological changes or even oxidative stress-dependent cell death. The reactive oxygen species (ROS) and malondialdehyde (MDA) were accumulated in the 1292-12 strain, while the antioxidant activities of intracellular catalase (CAT) and superoxide dismutase (SOD) were significantly decreased by 27.6 and 32.0%, respectively. Furthermore, it was also revealed that these issues could be ameliorated by the exogenous supplementation of vitamin C, fish and colza oil.https://www.mdpi.com/2309-608X/10/2/126lipidoxidative stressdelta-12 desaturaselinoleic acid<i>Yarrowia lipolytica</i> |
| spellingShingle | Yufei Chang Zhen Wang Hequn Li Wenrui Dang Yuanda Song Xinxin Kang Huaiyuan Zhang Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-Desaturase Journal of Fungi lipid oxidative stress delta-12 desaturase linoleic acid <i>Yarrowia lipolytica</i> |
| title | Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-Desaturase |
| title_full | Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-Desaturase |
| title_fullStr | Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-Desaturase |
| title_full_unstemmed | Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-Desaturase |
| title_short | Morphological Changes and Strong Cytotoxicity in <i>Yarrowia lipolytica</i> by Overexpressing Delta-12-Desaturase |
| title_sort | morphological changes and strong cytotoxicity in i yarrowia lipolytica i by overexpressing delta 12 desaturase |
| topic | lipid oxidative stress delta-12 desaturase linoleic acid <i>Yarrowia lipolytica</i> |
| url | https://www.mdpi.com/2309-608X/10/2/126 |
| work_keys_str_mv | AT yufeichang morphologicalchangesandstrongcytotoxicityiniyarrowialipolyticaibyoverexpressingdelta12desaturase AT zhenwang morphologicalchangesandstrongcytotoxicityiniyarrowialipolyticaibyoverexpressingdelta12desaturase AT hequnli morphologicalchangesandstrongcytotoxicityiniyarrowialipolyticaibyoverexpressingdelta12desaturase AT wenruidang morphologicalchangesandstrongcytotoxicityiniyarrowialipolyticaibyoverexpressingdelta12desaturase AT yuandasong morphologicalchangesandstrongcytotoxicityiniyarrowialipolyticaibyoverexpressingdelta12desaturase AT xinxinkang morphologicalchangesandstrongcytotoxicityiniyarrowialipolyticaibyoverexpressingdelta12desaturase AT huaiyuanzhang morphologicalchangesandstrongcytotoxicityiniyarrowialipolyticaibyoverexpressingdelta12desaturase |