Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-Indonesia

Endophytic bacteria are important microorganisms having potential as biocontrol agents for many pathogens. Until now, the growth of it always uses semi-synthetic or synthetic medium so it was difficult to be used by farmers in the field and it was expensive to have its propagation as biocontrol agen...

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Main Authors: Purnawati Arika, Harijani Wiwik Sri, Windriyanti Wiwin
Format: Article
Language:English
Published: EDP Sciences 2016-01-01
Series:MATEC Web of Conferences
Subjects:
Online Access:http://dx.doi.org/10.1051/matecconf/20165801016
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author Purnawati Arika
Harijani Wiwik Sri
Windriyanti Wiwin
author_facet Purnawati Arika
Harijani Wiwik Sri
Windriyanti Wiwin
author_sort Purnawati Arika
collection DOAJ
description Endophytic bacteria are important microorganisms having potential as biocontrol agents for many pathogens. Until now, the growth of it always uses semi-synthetic or synthetic medium so it was difficult to be used by farmers in the field and it was expensive to have its propagation as biocontrol agents. Based on the problem, this research will study the natural medium as propagation medium of Endophytic bacteria. It had natural ingredients such as soybean, chicken broth, egg, worms, snail, sorghum and they were easy to get by farmers. This study used endophytic bacteria from Solanaceae in Malang- Indonesia. Four isolates of endophytic bacteria were grown in agar and liquid medium with ingredients of corn flour, soybean flour, sorghum flour, snail flour, and worm flour. There is no difference in the incubation period, color, shape, and surface colony. The population in medium with snail flour ingredients at a concentration of 107 cfu/ml is the highest and snail flour is the best medium for growing endophytic bacteria.
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spelling doaj.art-970cb76dd53c42eea76277365b96cd092022-12-21T22:49:19ZengEDP SciencesMATEC Web of Conferences2261-236X2016-01-01580101610.1051/matecconf/20165801016matecconf_bisstech2016_01016Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-IndonesiaPurnawati Arika0Harijani Wiwik Sri1Windriyanti Wiwin2Faculty of Agricultural UPN “Veteran”Faculty of Agricultural UPN “Veteran”Faculty of Agricultural UPN “Veteran”Endophytic bacteria are important microorganisms having potential as biocontrol agents for many pathogens. Until now, the growth of it always uses semi-synthetic or synthetic medium so it was difficult to be used by farmers in the field and it was expensive to have its propagation as biocontrol agents. Based on the problem, this research will study the natural medium as propagation medium of Endophytic bacteria. It had natural ingredients such as soybean, chicken broth, egg, worms, snail, sorghum and they were easy to get by farmers. This study used endophytic bacteria from Solanaceae in Malang- Indonesia. Four isolates of endophytic bacteria were grown in agar and liquid medium with ingredients of corn flour, soybean flour, sorghum flour, snail flour, and worm flour. There is no difference in the incubation period, color, shape, and surface colony. The population in medium with snail flour ingredients at a concentration of 107 cfu/ml is the highest and snail flour is the best medium for growing endophytic bacteria.http://dx.doi.org/10.1051/matecconf/20165801016natural mediumendophytic bacteria
spellingShingle Purnawati Arika
Harijani Wiwik Sri
Windriyanti Wiwin
Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-Indonesia
MATEC Web of Conferences
natural medium
endophytic bacteria
title Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-Indonesia
title_full Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-Indonesia
title_fullStr Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-Indonesia
title_full_unstemmed Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-Indonesia
title_short Natural Medium for Growing of Endophytic Bacteria from Solanaceae in Malang-Indonesia
title_sort natural medium for growing of endophytic bacteria from solanaceae in malang indonesia
topic natural medium
endophytic bacteria
url http://dx.doi.org/10.1051/matecconf/20165801016
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