On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy
Bacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. For this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. However, phages are very host specific in comparison to wide-spectrum antibiotics and th...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-11-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01271/full |
| _version_ | 1818858342603292672 |
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| author | Sari eMattila Pilvi eRuotsalainen Matti eJalasvuori |
| author_facet | Sari eMattila Pilvi eRuotsalainen Matti eJalasvuori |
| author_sort | Sari eMattila |
| collection | DOAJ |
| description | Bacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. For this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. However, phages are very host specific in comparison to wide-spectrum antibiotics and thus preparation of phage-cocktails beforehand against pathogens can be difficult. In this study, we evaluate whether it may be possible to isolate phages on-demand from environmental reservoir. We attempted to enrich infectious bacteriophages from sewage against nosocomial drug-resistant bacterial strains of different medically important species in order to evaluate the probability of discovering novel therapeutic phages. Stability and host-range were determined for the acquired phages. Our results suggest that on-demand isolation of phages is possible against Pseudomonas aeruginosa, Salmonella and extended spectrum beta-lactamase (ESBL) Escherichia coli and Klebsiella pneumoniae. The probability of finding suitable phages was less than 40% against vancomycin resistant Enterococcus (VRE) and Acinetobacter baumannii strains. Furthermore, isolation of new phages against methicillin resistant Staphylococcus aureus (MRSA) strains was found to be very difficult. |
| first_indexed | 2024-12-19T08:54:46Z |
| format | Article |
| id | doaj.art-970d44985c2b4963a5e51ba08539eaf6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T08:54:46Z |
| publishDate | 2015-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-970d44985c2b4963a5e51ba08539eaf62022-12-21T20:28:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-11-01610.3389/fmicb.2015.01271160731On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapySari eMattila0Pilvi eRuotsalainen1Matti eJalasvuori2University of JyväskyläUniversity of JyväskyläUniversity of JyväskyläBacteriophages are bacterial viruses, capable of killing even multi-drug resistant bacterial cells. For this reason, therapeutic use of phages is considered as a possible alternative to conventional antibiotics. However, phages are very host specific in comparison to wide-spectrum antibiotics and thus preparation of phage-cocktails beforehand against pathogens can be difficult. In this study, we evaluate whether it may be possible to isolate phages on-demand from environmental reservoir. We attempted to enrich infectious bacteriophages from sewage against nosocomial drug-resistant bacterial strains of different medically important species in order to evaluate the probability of discovering novel therapeutic phages. Stability and host-range were determined for the acquired phages. Our results suggest that on-demand isolation of phages is possible against Pseudomonas aeruginosa, Salmonella and extended spectrum beta-lactamase (ESBL) Escherichia coli and Klebsiella pneumoniae. The probability of finding suitable phages was less than 40% against vancomycin resistant Enterococcus (VRE) and Acinetobacter baumannii strains. Furthermore, isolation of new phages against methicillin resistant Staphylococcus aureus (MRSA) strains was found to be very difficult.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01271/fullBacteriophagesantibiotic resistanceMRSAESBLphage therapyPhage cocktails |
| spellingShingle | Sari eMattila Pilvi eRuotsalainen Matti eJalasvuori On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy Frontiers in Microbiology Bacteriophages antibiotic resistance MRSA ESBL phage therapy Phage cocktails |
| title | On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy |
| title_full | On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy |
| title_fullStr | On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy |
| title_full_unstemmed | On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy |
| title_short | On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy |
| title_sort | on demand isolation of bacteriophages against drug resistant bacteria for personalized phage therapy |
| topic | Bacteriophages antibiotic resistance MRSA ESBL phage therapy Phage cocktails |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01271/full |
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